Reconstitution of proton pumping activity of a plasma membrane ATPase purified from radish

1985 ◽  
Vol 37 (3) ◽  
pp. 189-193 ◽  
Author(s):  
Maria Cecilia Cocucci ◽  
Maria Ida De Michelis ◽  
Maria Chiara Pugliarello ◽  
Franca Rasi-Caldogno
Keyword(s):  
Plasma Membrane ◽  
Proton Pumping ◽  
Plasma Membrane Atpase ◽  
Membrane Atpase ◽  
Pumping Activity

scholarly journals Eisosomes are metabolically regulated storage compartments for APC-type nutrient transporters

2018 ◽  
Vol 29 (17) ◽  
pp. 2113-2127 ◽  
Author(s):  
Akshay Moharir ◽  
Lincoln Gay ◽  
Daniel Appadurai ◽  
James Keener ◽  
Markus Babst
Keyword(s):  
Plasma Membrane ◽  
Cell Surface ◽  
Proton Pumping ◽  
Proton Gradient ◽  
Lipid Domains ◽  
Nutrient Transporters ◽  
Major Function ◽  
Show Evidence ◽  
Higher Eukaryotes ◽  
Pumping Activity

Eisosomes are lipid domains of the yeast plasma membrane that share similarities to caveolae of higher eukaryotes. Eisosomes harbor APC-type nutrient transporters for reasons that are poorly understood. Our analyses support the model that eisosomes function as storage compartments, keeping APC transporters in a stable, inactive state. By regulating eisosomes, yeast is able to balance the number of proton-driven APC transporters with the proton-pumping activity of Pma1, thereby maintaining the plasma membrane proton gradient. Environmental or metabolic changes that disrupt the proton gradient cause the rapid restructuring of eisosomes and results in the removal of the APC transporters from the cell surface. Furthermore, we show evidence that eisosomes require the presence of APC transporters, suggesting that regulating activity of nutrient transporters is a major function of eisosomes.

scholarly journals Two inhibitors of yeast plasma membrane ATPase 1 (ScPma1p): toward the development of novel antifungal therapies

2018 ◽  
Vol 10 (1) ◽  
Author(s):  
Sabine Ottilie ◽  
Gregory M. Goldgof ◽  
Andrea L. Cheung ◽  
Jennifer L. Walker ◽  
Edgar Vigil ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Plasma Membrane Atpase ◽  
Yeast Plasma Membrane ◽  
Membrane Atpase

scholarly journals Activation of yeast plasma membrane ATPase by phorbol ester

FEBS Letters ◽  
1985 ◽  
Vol 192 (1) ◽  
pp. 95-98 ◽  
Author(s):  
Francisco Portillo ◽  
María J. Mazón
Keyword(s):  
Plasma Membrane ◽  
Phorbol Ester ◽  
Plasma Membrane Atpase ◽  
Yeast Plasma Membrane ◽  
Membrane Atpase
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