A comparative study on the effects of ascorbic acid deficiency and supplementation on endurance and mitochondrial oxidative capacities in various tissues of the guinea pig

1986 ◽  
Vol 83 (1) ◽  
pp. 235-240 ◽  
Author(s):  
L. Packer ◽  
K. Gohil ◽  
B. DeLumen ◽  
S.E. Terblanche
Keyword(s):  
Ascorbic Acid ◽  
Guinea Pig ◽  
Comparative Study ◽  
Ascorbic Acid Deficiency ◽  
Acid Deficiency

L-Ascorbic acid and lysosomal acid hydrolase activities of guinea pig liver and brain

1978 ◽  
Vol 56 (5) ◽  
pp. 352-356 ◽  
Author(s):  
Susan K. Hoehn ◽  
Julian N. Kanfer
Keyword(s):  
Ascorbic Acid ◽  
Acid Phosphatase ◽  
Guinea Pig ◽  
Lysosomal Hydrolases ◽  
Vitro Assay ◽  
Ascorbic Acid Deficiency ◽  
Acid Deficiency ◽  
Dietary Treatments ◽  
The Brain

The effects of L-ascorbic acid deficiency on guinea pig hepatic and brain lysosomal hydrolases were examined. In general, hepatic β-N-acetylhexosaminidase, β-D-glucoronidase, α-D-galactosidase, α-D-mannosidase, and acid phosphatase were elevated in scorbutic animals. This appears to be independent of the starved state. Brain β-D-glucoronidase and acid phosphatase followed a similar pattern to that observed with the liver enzymes, but brain β-N-acetylhexosaminidase was not affected by L-ascorbic acid deficiency. Supplementing the in vitro assay system with L-ascorbic acid decreased the activity of hepatic β-N-acetylhexosaminidase somewhat but had no effect on the brain enzyme. Serum total β-N-acetylhexosaminidase was unaffected by dietary treatments although the activity of β-N- acetylhexosaminidase A tended to increase in the scorbutic animals. Subcellular fractions were obtained from the three groups of animals and the recoveries of protein, β-N-acetylhexosaminidase, and glucose-6-phosphatase estimated.

scholarly journals Effect of pharmacological doses of ascorbic acid on the hepatic microsomal haemoproteins in the guinea-pig

1983 ◽  
Vol 49 (1) ◽  
pp. 27-33 ◽  
Author(s):  
Judith L. Sutton ◽  
T. K. Basu ◽  
J. W. T. Dickerson
Keyword(s):  
Ascorbic Acid ◽  
Guinea Pig ◽  
Guinea Pigs ◽  
Sucrose Solution ◽  
Significant Rise ◽  
Mixed Function Oxidase ◽  
Ascorbic Acid Deficiency ◽  
Acid Deficiency ◽  
Specific Activities ◽  
Endogenous Substrates

1. Ascorbic acid deficiency results in a reduction in the activity of the hepatic mixed function oxidase systems in the guinea-pig. In this study, male Dunkin-Hartley guinea-pigs were given 0, 50, 100, 200 or 300 mg ascorbic acid/d in two equal doses in buffered sucrose solution (200 g/l) for 4 d. Controls received an equal volume of sucrose solution.2. A dose of 50 mg ascorbic acid/d resulted in a significant rise in the specific activities of both cytochromes P-450 and b5. At doses of 200 and 300 mg ascorbic acid/d the concentration of both haemoproteins was significantly lower than the control values. These effects were mirrored by total microsomal haem concentration.3. These results suggest that when given in large doses, ascorbic acid ceases to act simply as a vitamin and should be considered a drug competing for substrates and cofactors with co-administered drugs and endogenous substrates such as cholesterol.

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