1035 Contralateral effects following ipsilateral pulmonary radiation: Gene expression and growth factor release

1995 ◽  
Vol 32 ◽  
pp. 240
Author(s):  
Philip Rubin ◽  
Sandra McDonald ◽  
Jacqueline Williams ◽  
Christine Reed ◽  
Jacob Finkelstein
Keyword(s):  
Gene Expression ◽  
Growth Factor ◽  
Growth Factor Release ◽  
Contralateral Effects ◽  
Factor Release

scholarly journals Does Platelet-Rich Plasma Freeze-Thawing Influence Growth Factor Release and Their Effects on Chondrocytes and Synoviocytes?

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Alice Roffi ◽  
Giuseppe Filardo ◽  
Elisa Assirelli ◽  
Carola Cavallo ◽  
Annarita Cenacchi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Growth Factor ◽  
Culture Media ◽  
Platelet Rich Plasma ◽  
Secreted Proteins ◽  
Safe Procedure ◽  
Thawing Cycle ◽  
Growth Factor Release ◽  
Similar Gene ◽  
Factor Release

PRP cryopreservation remains a controversial point. Our purpose was to investigate the effect of freezing/thawing on PRP molecule release, and its effects on the metabolism of chondrocytes and synoviocytes. PRP was prepared from 10 volunteers, and a half volume underwent one freezing/thawing cycle. IL-1β, HGF, PDGF AB/BB, TGF-β1, and VEGF were assayed 1 hour and 7 days after activation. Culture media of chondrocytes and synoviocytes were supplemented with fresh or frozen PRP, and, at 7 days, proliferation, gene expression, and secreted proteins levels were evaluated. Results showed that in the freeze-thawed PRP the immediate and delayed molecule releases were similar or slightly lower than those in fresh PRP. TGF-β1 and PDGF AB/BB concentrations were significantly reduced after freezing both at 1 hour and at 7 days, whereas HGF concentration was significantly lower in frozen PRP at 7 days. In fresh PRP IL-1βand HGF concentrations underwent a significant further increase after 7 days. Similar gene expression was found in chondrocytes cultured with both PRPs, whereas in synoviocytes HGF gene expression was higher in frozen PRP. PRP cryopreservation is a safe procedure, which sufficiently preserves PRP quality and its ability to induce proliferation and the production of ECM components in chondrocytes and synoviocytes.

Growth factor release from platelet concentrates: analytic quantification and characterization for clinical applications

Vox Sanguinis ◽  
2013 ◽  
Vol 105 (2) ◽  
pp. 129-136 ◽  
Author(s):  
C. Durante ◽  
F. Agostini ◽  
L. Abbruzzese ◽  
R. T. Toffola ◽  
S. Zanolin ◽  
...  
Keyword(s):  
Growth Factor ◽  
Clinical Applications ◽  
Platelet Concentrates ◽  
Growth Factor Release ◽  
Factor Release

scholarly journals Effects of the breed, sex and age on cellular content and growth factor release from equine pure-platelet rich plasma and pure-platelet rich gel

2013 ◽  
Vol 9 (1) ◽  
pp. 29 ◽  
Author(s):  
Carlos E Giraldo ◽  
Catalina López ◽  
María E Álvarez ◽  
Ismael J Samudio ◽  
Marta Prades ◽  
...  
Keyword(s):  
Growth Factor ◽  
Platelet Rich Plasma ◽  
Cellular Content ◽  
Growth Factor Release ◽  
Factor Release
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