Retinoic acid-induced increase in delta-opioid receptor and N-Methyl-d-Aspartate receptor mRNA levels in neuroblastoma × glioma (NG108-15) cells

1996 ◽  
Vol 39 (3) ◽  
pp. 193-199 ◽  
Author(s):  
Iwona W. Beczkowska ◽  
Jochen Buck ◽  
Charles E. Inturrisi
1994 ◽  
Vol 39 (6) ◽  
pp. 674-679 ◽  
Author(s):  
B. Kest ◽  
S. Jenab ◽  
M. Brodsky ◽  
K. Elliott ◽  
C. E. Inturrisi

Analgesia ◽  
1995 ◽  
Vol 1 (3) ◽  
pp. 195-200 ◽  
Author(s):  
Benjamin Kest ◽  
Shirzad Jenab ◽  
Charles E. Inturrisi

2002 ◽  
Vol 58 (3) ◽  
pp. 285-288 ◽  
Author(s):  
Eugene D Festa ◽  
Christine Cecala ◽  
Vanya Quinones-Jenab ◽  
Shirzad Jenab

1999 ◽  
Vol 816 (2) ◽  
pp. 381-389 ◽  
Author(s):  
Benjamin Kest ◽  
Shirzad Jenab ◽  
Marina Brodsky ◽  
Bogdan Sadowski ◽  
John K. Belknap ◽  
...  

1995 ◽  
Vol 33 (2) ◽  
pp. 351-355 ◽  
Author(s):  
Ellen M. Unterwald ◽  
Joshua M. Rubenfeld ◽  
Yasuo Imai ◽  
Jia-Bei Wang ◽  
George R. Uhl ◽  
...  

2000 ◽  
Vol 25 (1) ◽  
pp. 53-61 ◽  
Author(s):  
M Hattori ◽  
K Takesue ◽  
N Nishida ◽  
Y Kato ◽  
N Fujihara

The present study investigated the effect of retinoic acid (RA) on the differentiation of granulosa cells prepared from porcine ovaries. The granulosa cells were precultured for 15 h, then cultured for 48 h with FSH and further treated for 24 h with LH in order to induce their transformation into luteal cells. After the cells had been exposed to 1 microM retinoids (RA, retinal and retinol) for 87 h, analysis of the LH receptor mRNA expression, an indicator of granulosa cell differentiation, was carried out by using semiquantitative RT-PCR. The results showed that there was a decrease in LH receptor mRNA levels, and that RA had a more potent effect on these levels than the other two retinoids. When cells were exposed to RA in the immature stage (before the addition of FSH) or the early stage of development (0-24 h after the addition of FSH), expression of LH receptor mRNA was greatly diminished. When the immature cells were cultured for 15 h with RA, then washed and cultured for 48 h with FSH and for 24 h with LH, the expression of LH receptor mRNA was not reversed. In the differentiated cells (24 h after the addition of FSH), however, RA no longer had any inhibitory effect. When the immature cells were exposed to RA, FSH-induced expression of c-fos mRNA was markedly decreased. In contrast, expression of c-jun and activating transcription factor-4 mRNAs remained constant. However, the expression of c-fos mRNA was not decreased by forskolin. The results indicate that RA is a potent inhibitor in the immature stage of porcine granulosa cell differentiation, probably through decreased expression of FSH receptor, but that RA does not inhibit differentiation in the mature stage of the cells.


1997 ◽  
Vol 45 (1) ◽  
pp. 149-153 ◽  
Author(s):  
M.Paola Castelli ◽  
Miriam Melis ◽  
Michela Mameli ◽  
Paola Fadda ◽  
Giacomo Diaz ◽  
...  

2002 ◽  
Vol 71 (4) ◽  
pp. 496-503 ◽  
Author(s):  
Anders I. Persson ◽  
N. David Åberg ◽  
Jan Oscarsson ◽  
Olle G.P. Isaksson ◽  
Lars Rönnbäck ◽  
...  

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