A collaborative in vitro dissolution study: comparing the flow-through method with the USP paddle method using USP prednisone calibrator tablets

The objective of present study was to develop colon targeted drug delivery using bacterially triggered approach through oral route. Valdecoxib (COX-2 inhibitor) was chosen as a model drug in order to target it to colon which may prove useful in inflammatory bowel disease and related disorders. Matrix tablets of Valdecoxib were prepared by wet granulation technique utilizing different ratio of Guar gum and Sodium starch glycholate. The prepared matrix tablets were evaluated for uniformity of weight, uniformity of content, hardness and in vitro dissolution study in simulated gastric and intestinal fluid (Phosphate Buffer pH-1.2, pH-6.8 and pH-7.4), followed by Dissolution study in bio-relevant dissolution media Phosphate Buffer (pH-6.8) containing rat caecal content. The results revealed that the formulated batch had released lesser quantity of drug at pH 1.2 and pH 7.4 in 2 hors whereas in biorelevent dissolution media containing rat caecal content it released significantly higher amount of drug which was also significantly higher than the dissolution media of same pH without caecal content (microflora) and it was concluded that guar gum can be used as a potential carrier for targeting drugs to colon.

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In Vitro Dissolution Testing with Flow-Through Method: A Technical Note

AAPS PharmSciTech ◽

10.1208/s12249-009-9339-6 ◽

2009 ◽

Vol 10 (4) ◽

Cited By ~ 16

Author(s):

Zongming Gao

Keyword(s):

Technical Note ◽

In Vitro Dissolution ◽

Dissolution Testing ◽

Flow Through

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In vitro dissolution study of plutonium in aerosol particles from the Mayak PA: a tool for individualised dose estimates

Radiation Protection Dosimetry ◽

10.1093/rpd/ncm340 ◽

2007 ◽

Vol 127 (1-4) ◽

pp. 60-63 ◽

Cited By ~ 12

Author(s):

E. E. Aladova ◽

S. A. Romanov ◽

R. A. Guilmette ◽

V. F. Khokhryakov ◽

K. G. Suslova

Keyword(s):

Aerosol Particles ◽

In Vitro Dissolution ◽

Dissolution Study

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EFFECT OF DRUG TO B-CYCLODEXTRIN RATIO AND METHOD OF COMPLEXATION IN THE DEVELOPMENT OF B-CYCLODEXTRIN INCLUSION COMPLEX OF OFLOXACIN

INDIAN DRUGS ◽

10.53879/id.50.12.p0034 ◽

2013 ◽

Vol 50 (12) ◽

pp. 34-40

Author(s):

M Panchpuri ◽

◽

D Singh ◽

A Semalty ◽

M. Semalty

Keyword(s):

Inclusion Complex ◽

In Vitro Dissolution ◽

Molar Ratio ◽

Dissolution Profile ◽

Scanning Calorimetry ◽

Dissolution Study ◽

Drug Content ◽

Kneading Method ◽

Made In

Ofloxacin, a second generation fluoroquinolone, shows poor aqueous solubility and dissolution profile. Thus, ofloxacin–β-cyclodextrin complexes were prepared to improve its dissolution by imparting an environment of improved hydrophilicity. Ofloxacin was complexed with β-cyclodextrin (in 1:1 and 1:2 molar ratio) by two different methods namely, solvent evaporation and kneading method. These inclusion complexes were evaluated for solubility, drug content, scanning electron microscopy (SEM), differential scanning calorimetry (DSC), X ray powder diffraction (XRPD) and in vitro dissolution study. The highest drug content (35.45%) was found in complex made by kneading method (OK1:1) in 1:1 molar ratio. All the complexes OSE1:1, OSE1:2, OK1:1, OK1:2 were found to be showing rough and porous surface morphology in SEM. Solubility as well as the dissolution of the complexes was found to be improved. Complex prepared by kneading method in 1:1 molar ratio (OK1:1) showed a marked improvement in percent drug release (88.94%) than that of pure drug (54.22%) at the end of 1 hour in dissolution study. FTIR, DSC and XRPD data confirmed the formation of inclusion complex. It was concluded that the complex made in 1:1 molar ratio (irrespective of the method) showed better solubility and dissolution profile as compared to complex made in 1:2 molar ratio.

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In vitro dissolution study on inclusion complex of piperine with ethylenediamine-β-cyclodextrin

Journal of Inclusion Phenomena and Macrocyclic Chemistry ◽

10.1007/s10847-020-00980-5 ◽

2020 ◽

Vol 96 (3-4) ◽

pp. 233-243 ◽

Cited By ~ 1

Author(s):

Kai Liu ◽

Huijun Liu ◽

Zhendong Li ◽

Wei Li ◽

Liuxing Li

Keyword(s):

Inclusion Complex ◽

In Vitro Dissolution ◽

Dissolution Study

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DEVELOPMENT AND EVALUATION OF NOVEL ESTIMATION TECHNIQUES FOR IN VITRO DISSOLUTION STUDY AND VALIDATION PROTOCOL FOR ESCITALOPRAM AS ANTIDEPRESSANT DRUG AND THEIR FORMULATION

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2020v12i8.37964 ◽

2020 ◽

pp. 55-61

Author(s):

SHRIRAM H. BAIRAGI ◽

R. S. GHOSH

Keyword(s):

Limit Of Detection ◽

Antidepressant Drug ◽

Correlation Factor ◽

In Vitro Dissolution ◽

Linear Calibration ◽

Limit Of Quantification ◽

Minimum Concentration ◽

Dissolution Study ◽

Relative Standard

Objective: To develop and validate the RP-HPLC method and in vitro dissolution study for escitalopram as antidepressant drug and their formulation. Methods: The chromatographic separation was done by using a C-18, 150 mm column and a mobile phase consisting of phosphate buffer (40%) and acetonitrile HPLC grade (60%). Detection was carried out at 211 nm with a flow rate of 1 ml/min with an injection of 20 μl. The method was validated with different parameters such as linearity, precision, accuracy, robustness, and limit of detection (LOD), the limit of quantification (LOQ) according to ICH guidelines. Results: The linear calibration curve was obtained in the concentration range of 0-50 μg/ml and gave an average correlation factor 0.992. The retention time was observed at 2.96 min. The Minimum concentration level at which the analyte can be reliably detected (LOD) and quantified (LOQ) were found to be 0.03 and 0.09 µg/ml, respectively. The relative standard deviation of intra and the inter-day assay was found to be less than 2. The dissolution studies show moderate dissolution (23.4%) after 45 min, but it reaches a plateau after approximately 25 min. Conclusion: This method was found to be simple, rapid and economic with less run time. The validated parameters manifest the method is reliable, linear, accurate and precise as well as robust with minor variations in chromatographic parameters. Therefore, the developed method can be applied for both routine analysis and quality control assay and it could be a very powerful tool to investigate the stability of escitalopram.

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