Comparison of cell culture with an immunoperoxidase kit for rapid diagnosis of herpes simplex virus infections

1984 ◽  
Vol 2 (4) ◽  
pp. 343-345 ◽  
Author(s):  
V.C. Salmon ◽  
Barbara K. Michaels ◽  
Ronald B. Turner
Keyword(s):  
Cell Culture ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Rapid Diagnosis ◽  
Virus Infections ◽  
Simplex Virus

scholarly journals Evaluation of LightCycler PCR for Implementation of Laboratory Diagnosis of Herpes Simplex Virus Infections

2000 ◽  
Vol 38 (8) ◽  
pp. 3116-3118 ◽  
Author(s):  
Mark J. Espy ◽  
Teri K. Ross ◽  
Rosaline Teo ◽  
Kathleen A. Svien ◽  
Arlo D. Wold ◽  
...  
Keyword(s):  
Cell Culture ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Dna Polymerase ◽  
Laboratory Diagnosis ◽  
Routine Laboratory ◽  
Virus Infections ◽  
Shell Vial ◽  
Simplex Virus ◽  
Positive Results

Five hundred specimens (288 genital, 192 dermal, and 20 ocular) were extracted by technologists, and the DNA was assayed by LightCycler PCR (DNA polymerase and thymidine kinase [TK] gene targets) and by conventional tube and shell vial cell culture. One hundred fifty-eight confirmed (by cell culture and TK target PCR) positive and LightCycler-positive specimens were detected during the first 30 PCR cycles. LightCycler PCR-positive results for cycles 31 to 45 (39 of 67 [58.2%]) required confirmation by another PCR target (TK). LightCycler PCR is more sensitive (n = 197; 23.1%) than cell cultures (n = 150) for the routine laboratory detection of herpes simplex virus infections.

Rapid Diagnosis of Herpes Simplex Virus Infections with Fluorescent Antibody

Science ◽  
1959 ◽  
Vol 129 (3349) ◽  
pp. 640-641 ◽  
Author(s):  
J. Z. BIEGELEISEN ◽  
L. V. SCOTT ◽  
V. LEWIS
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Fluorescent Antibody ◽  
Rapid Diagnosis ◽  
Virus Infections ◽  
Simplex Virus
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