Diagnostic value of rapid detection of Chlamydia trachomatis by using amplified enzyme immunoassay in infants with respiratory infections

1993 ◽  
Vol 17 (3) ◽  
pp. 233-234 ◽  
Author(s):  
Kei Numazaki ◽  
Shunzo Chiba
Keyword(s):  
Chlamydia Trachomatis ◽  
Enzyme Immunoassay ◽  
Rapid Detection ◽  
Respiratory Infections ◽  
Diagnostic Value

scholarly journals Diagnostic Value of Enzyme Immunoassay (Chlamydiazyme) for the Diagnosis of Chlamydia trachomatis Infections in Uterine Cervix

1988 ◽  
Vol 62 (11) ◽  
pp. 1002-1009
Author(s):  
Yoshio SHIINA ◽  
Junko IIJIMA ◽  
Yoshiaki SAWADA ◽  
Tadao IWAKURA ◽  
Hiroshi MIYAZAWA ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Enzyme Immunoassay ◽  
Uterine Cervix ◽  
Diagnostic Value

Rapid detection of Chlamydia trachomatis by an enzyme immunoassay method

1986 ◽  
Vol 5 (3) ◽  
pp. 225-234 ◽  
Author(s):  
Raymond W. Ryan ◽  
Irene Kwasnik ◽  
Olafur Steingrimsson ◽  
Johannes Gudmundsson ◽  
Hannes Thorarinsson ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Enzyme Immunoassay ◽  
Rapid Detection

scholarly journals Chlamydia trachomatis intra-bacterial and total plasmid copy number in clinical urogenital samples

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
J. A. M. C. Dirks ◽  
K. Janssen ◽  
C. J. P. A. Hoebe ◽  
T. H. B. Geelen ◽  
M. Lucchesi ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Diagnostic Value ◽  
Plasmid Copy Number ◽  
Extracellular Dna ◽  
Chromosomal Dna ◽  
Plasmid Copy ◽  
Clinical Sensitivity ◽  
Copy Numbers ◽  
Vaginal Swabs ◽  
And Gender

AbstractChlamydia trachomatis (CT) increases its plasmid numbers when stressed, as occurs in clinical trachoma samples. Most CT tests target the plasmid to increase the test sensitivity, but some only target the chromosome. We investigated clinical urogenital samples for total plasmid copy numbers to assess its diagnostic value and intra-bacterial plasmid copy numbers to assess its natural variation. Both plasmid and chromosome copies were quantified using qPCR, and the plasmid:chromosome ratio (PCr) calculated in two cohorts: (1) 383 urogenital samples for the total PCR (tPCr), and (2) 42 vaginal swabs, with one half treated with propium-monoazide (PMA) to prevent the quantification of extracellular DNA and the other half untreated to allow for both tPCr and intra-bacterial PCr (iPCr) quantification. Mann–Whitney U tests compared PCr between samples, in relation to age and gender. Cohort 1: tPCr varied greatly (1–677, median 16). Median tPCr was significantly higher in urines than vaginal swabs (32 vs. 11, p < 0.001). Cohort 2: iPCr was more stable than tPCr (range 0.1–3 vs. 1–11). To conclude, tPCr in urogenital samples was much more variable than previously described. Transport time and temperature influences DNA degradation, impacting chromosomal DNA more than plasmids and urine more than vaginal samples. Data supports a plasmid target in CT screening assays to increase clinical sensitivity.

PCR of DNA from dried blood spots on filter paper coupled to a simple DNA enzyme immunoassay for rapid detection of HIV-1

1996 ◽  
Vol 52 (4) ◽  
pp. 308-309
Author(s):  
B. Schweiger ◽  
C. Kücherer ◽  
C. Fleischer ◽  
H. v. Spreckelsen ◽  
P. Zablocki-Kaiser ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Filter Paper ◽  
Rapid Detection ◽  
Dried Blood Spots ◽  
Blood Spots ◽  
Dna Enzyme Immunoassay ◽  
Hiv 1
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