Natural adhesion of Saccharomyces cerevisiae onto sugar beet pulp (SBP) is a
very simple and cheap immobilization method for retaining high cells density
in the ethanol fermentation system. In the present study, yeast cells were
immobilized by adhesion onto SBP suspended in the synthetic culture media
under different conditions such as: glucose concentration (100, 120 and 150
g/l), inoculum concentration (5, 10 and 15 g/l dry mass) and temperature (25,
30, 35 and 40?C). In order to estimate the optimal immobilization conditions
the yeast cells retention (R), after each immobilization experiment was
analyzed. The highest R value of 0.486 g dry mass yeast /g dry mass SBP was
obtained at 30?C, glucose concentration of 150 g/l, and inoculum
concentration of 15 g/l. The yeast immobilized under these conditions was
used for ethanol fermentation of sugar beet molasses containing 150.2 g/l of
reducing sugar. Efficient ethanol fermentation (ethanol concentration of
70.57 g/l, fermentation efficiency 93.98%) of sugar beet molasses was
achieved using S. cerevisiae immobilized by natural adhesion on SBP.
Influence of inoculum to substrate ratio on methane production in Biochemical Methane Potential (BMP) tests of sugarcane distillery waste water
