scholarly journals Influence of inoculum to substrate ratio on methane production in Biochemical Methane Potential (BMP) tests of sugarcane distillery waste water

2019 ◽  
Vol 35 ◽  
pp. 259-264 ◽  
Author(s):  
Helene Caillet ◽  
Edouard Lebon ◽  
Esther Akinlabi ◽  
Daniel Madyira ◽  
Laetitia Adelard
2012 ◽  
Vol 512-515 ◽  
pp. 444-448 ◽  
Author(s):  
Sumeth Dechrugsa ◽  
Sumate Chaiprapat

The effects of substrate mix ratio and inoculum/substrate ratio (ISR) on biochemical methane potential of para-grass (PG) and pig manure (PM) were investigated in batch test that maintained temperature at 35±1 oC and continuously shaked at 120 rpm. Cumulative methane production data at different mix ratios and ISRs were evaluated and fitted with Gompertz equation to derive methane production potential (mL) and maximum methane production rate (mL/d). The maximum and average methane yields at each respective ISR of 1, 2, 3, and 4 were [413.0, 315.5], [539.7, 455.6], [590.3, 472.5], and [593.1, 473.5] mL/gVSadded. Relationship of ISR and PG mix ratio to specific methane yield were expressed in quadratic regression model. The generated response surface showed that methane yield was elevated at higher ISR and higher PG mix ratio. This suggests a careful investigation at different ISR and substrate mix ratios should be performed in order to develop a realistic biochemical methane potential of anaerobic co-digestion.


2011 ◽  
Vol 64 (4) ◽  
pp. 880-886 ◽  
Author(s):  
P. D. Jensen ◽  
H. Ge ◽  
D. J. Batstone

The biodegradability and bioavailability of hydrolysis-limited substrates under anaerobic (and aerobic) conditions can be represented by two key parameters – degradability (fd), or the percentage that can be effectively be destroyed during digestion, and first order hydrolysis coefficient (khyd), or the speed at which material breaks down. Biochemical methane potential (BMP) testing uses a batch test (in triplicate), and by fitting against a first order model, can fit both parameters in the same test. BMP testing is now being widely used for anaerobic process feasibility and design purposes, and standardisation efforts are ongoing. In this paper, we address a number of key issues relating to the test method and its analysis. This includes proposal of a new fitting and parameter estimation method, evaluation of the impact of inoculum to substrate ratio on fitted parameters, and comparison to performance in continuous systems. The new parameter estimation technique provides an estimate of parameter uncertainty and correlation, and is clearly more suitable than model transformation and linear regression. An inoculum volume ratio of at least 50% (2:1 on VS basis) was required on a cellulose substrate to use methane production as primary indicator, as found by comparing methane production and solubilisation of cellulose. Finally, on a typical material, waste activated sludge, the batch test was slightly conservative in terms of degradability and rate, indicating a bias in the BMP test. The test is a cost-effective and capable method to evaluate potential substrates, but it should be noted that it is generally conservative, especially if sub-optimal inoculum is used.


Water ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 2814 ◽  
Author(s):  
Thierry Ribeiro ◽  
Romain Cresson ◽  
Sébastien Pommier ◽  
Sébastien Preys ◽  
Laura André ◽  
...  

Biochemical methane potential (BMP) is essential to determine the production of methane for various substrates; literature shows important discrepancies for the same substrates. In this paper, a harmonized BMP protocol was developed and tested with two phases of BMP tests carried out by eleven French laboratories. Surprisingly, for the three same solid tested substrates (straw; raw mix and dried-shredded mix of potatoes, maize, beef meat and straw; and mayonnaise), the standard deviations of the repeatability and reproducibility inter-laboratory were not enhanced by the harmonized protocol (average of about 25% depending on the substrate), as compared to a previous step where all laboratories used their own protocols. Moreover, statistical analyses of all the results, after removal of the outliers (about 15% of all observations), did not highlight significant effect of the operational effect on BMP (stirring, automatic or manual gas quantification, use of trace metal, uses a bicarbonate buffer, inoculum to substrate ratio) at least for the tested ranges. On the other hand, the average intra-laboratory repeatability was low, about 7%, whatever the protocol, the substrate and the laboratory. It also appears that drying the SA substrate, which contained proteins, carbohydrates, lipids and fibers, does not impact its BMP.


Author(s):  
Kessia Caroline Dantas da Silva ◽  
Miriam Cleide Cavalcante de Amorim ◽  
Renan Santana Galvão ◽  
Yandra Beatriz de Oliveira Gonçalves ◽  
Paula Tereza de Souza e Silva ◽  
...  

The grape industrialization process produces large volumes of solid organic waste, with the grape bagasse being the main waste generated in the winemaking process. Anaerobic digestion can be used to treat and dispose of agro-industrial biomass waste. The objective of this study was to evaluate the effect of the inoculum/substrate ratio on the Biochemical Methane Potential (BMP) of grape marc. The experiment was performed in laboratory scale through a system of reactor bottles in batches, removing a set of triplicate flasks for sampling and analysis every 48 hours, with the test lasting 12 days. The reactors contained residue, inoculum and 20% of nutritive solution, maintaining 20% of headspace. The reactors were incubated in an incubator at a mesophilic temperature (35 ± 2°C) and shaken manually every 24 hours. Three different inoculum/substrate (I/S) ratios of 0.75, 1.5 and 3 were used to evaluate the methane yield, organic removals and at the end of degradation the morphology of the bacterial community was evaluated by means of scanning electron microscopy. The I/S 3 ratio provided the best results for loading anaerobic systems, indicating that grape marc presents potential for biological treatment through anaerobic digestion.


2018 ◽  
Vol 10 (8) ◽  
pp. 2678 ◽  
Author(s):  
Bilhate Chala ◽  
Hans Oechsner ◽  
Sajid Latif ◽  
Joachim Müller

Primary coffee processing is performed following the dry method or wet method. The dry method generates husk as a by-product, while the wet method generates pulp, parchment, mucilage, and waste water. In this study, characterization, as well as the potential of husk, pulp, parchment, and mucilage for methane production were examined in biochemical methane potential assays performed at 37 °C. Pulp, husk, and mucilage had similar cellulose contents (32%). The lignin contents in pulp and husk were 15.5% and 17.5%, respectively. Mucilage had the lowest hemicellulose (0.8%) and lignin (5%) contents. The parchment showed substantially higher lignin (32%) and neutral detergent fiber (96%) contents. The mean specific methane yields from husk, pulp, parchment, and mucilage were 159.4 ± 1.8, 244.7 ± 6.4, 31.1 ± 2.0, and 294.5 ± 9.6 L kg−1 VS, respectively. The anaerobic performance of parchment was very low, and therefore was found not to be suitable for anaerobic fermentation. It was estimated that, in Ethiopia, anaerobic digestion of husk, pulp, and mucilage could generate as much as 68 × 106 m3 methane per year, which could be converted to 238,000 MWh of electricity and 273,000 MWh of thermal energy in combined heat and power units. Coffee processing facilities can utilize both electricity and thermal energy for their own productive purposes.


Energies ◽  
2021 ◽  
Vol 14 (8) ◽  
pp. 2085
Author(s):  
Yang Mo Gu ◽  
Seon Young Park ◽  
Ji Yeon Park ◽  
Byoung-In Sang ◽  
Byoung Seong Jeon ◽  
...  

The impact of attrition ball-mill pretreatment on food waste particle size, soluble chemical oxygen demand (SCOD), biochemical methane potential, and microbial community during anaerobic digestion was investigated based on milling speed and time. The uniformity of particle size improved with increasing milling speed and time. The SCOD of the pretreated samples increased to 4%, 7%, and 17% at the speeds of 150, 225, and 300 rpm, respectively, compared to the control. Milling time did not significantly change the SCOD. The cumulative methane productions of 430, 440, and 490 mL/g-VS were observed at the speeds of 150, 225, and 300 rpm, respectively, while the untreated sample exhibited the cumulative methane production of 390 mL/g-VS. Extended milling time did not improve methane production much. When the milling times of 10, 20, and 30 min were applied with the milling speed fixed at 300 rpm, the methane productions of 490, 510, and 500 mL/g-VS were observed respectively. Ball-mill pretreatment also increased the total volatile fatty acids. During the anaerobic digestion (AD) of ball-mill treated food waste, acetoclastic methanogens predominated, with a relative abundance of 48–49%. Interestingly, hydrogenotrophic methanogens were 1.6 times higher in the pretreated samples than those in the control. These results showed the potential of attrition ball milling as a food waste pretreatment for improving methane production.


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