Nitric oxide synthase; Immunolocalisation and function in normal human skin

The nitric oxide synthase-like protein fromBacillus cereus(bcNOS) has been cloned, expressed, and characterized. This small hemeprotein (356 amino acids in length) has a mass of 43 kDa and forms a dimer. The recombinant protein showed similar spectral shifts to the mammalian NOS proteins and could bind the substrates L-arginine andNG-hydroxy-L-arginine as well as the ligand imidazole. Low levels of activity were recorded for the hydrogen peroxide-dependent oxidation ofNG-hydroxy-L-arginine and L-arginine by bcNOS, while a reconstituted system with the rat neuronal NOS reductase domain showed no activity. The recombinant bcNOS protein adds to the complement of bacterial NOS-like proteins that are used for the investigation of the mechanism and function of NO in microorganisms.

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Inhibitors of Nitric Oxide Synthase in Human Skin

Journal of Investigative Dermatology ◽

10.1111/1523-1747.ep12328204 ◽

1996 ◽

Vol 106 (1) ◽

pp. 113-118 ◽

Cited By ~ 70

Author(s):

Portia C. Goldsmith ◽

Tabi A. Leslie ◽

Nicholas A. Hayes ◽

Nicholas J. Levell ◽

Pauline M. Dowd ◽

...

Keyword(s):

Nitric Oxide ◽

Nitric Oxide Synthase ◽

Human Skin ◽

Oxide Synthase

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Regulation and function of the protein inhibitor of nitric oxide synthase (PIN)/dynein light chain 8 (LC8) in a human mast cell line

Life Sciences ◽

10.1016/j.lfs.2006.11.025 ◽

2007 ◽

Vol 80 (10) ◽

pp. 959-964 ◽

Cited By ~ 6

Author(s):

Scott D. McCauley ◽

Mark Gilchrist ◽

A. Dean Befus

Keyword(s):

Nitric Oxide ◽

Mast Cell ◽

Nitric Oxide Synthase ◽

Light Chain ◽

Human Mast Cell ◽

Dynein Light Chain ◽

Protein Inhibitor ◽

Mast Cell Line ◽

And Function ◽

Oxide Synthase

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Expression of Inducible Nitric Oxide Synthase in Skin Lesions of Patients with American Cutaneous Leishmaniasis

Infection and Immunity ◽

10.1128/iai.70.8.4638-4642.2002 ◽

2002 ◽

Vol 70 (8) ◽

pp. 4638-4642 ◽

Cited By ~ 53

Author(s):

Muna Qadoumi ◽

Inge Becker ◽

Norbert Donhauser ◽

Martin Röllinghoff ◽

Christian Bogdan

Keyword(s):

Nitric Oxide ◽

Nitric Oxide Synthase ◽

Cutaneous Leishmaniasis ◽

Leishmania Donovani ◽

Leishmania Major ◽

Parasite Burden ◽

Skin Lesions ◽

And Function ◽

Oxide Synthase

ABSTRACT Cytokine-inducible (or type 2) nitric oxide synthase (iNOS) is indispensable for the resolution of Leishmania major or Leishmania donovani infections in mice. In contrast, little is known about the expression and function of iNOS in human leishmaniasis. Here, we show by immunohistological analysis of skin biopsies from Mexican patients with local (LCL) or diffuse (DCL) cutaneous leishmaniasis that the expression of iNOS was most prominent in LCL lesions with small numbers of parasites whereas lesions with a high parasite burden (LCL or DCL) contained considerably fewer iNOS-positive cells. This is the first study to suggest an antileishmanial function of iNOS in human Leishmania infections in vivo.

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