Immunostaining of human amniotic epithelial cells: Possible use as a transgene carrier in gene therapy for inborn errors of metabolism

1995 ◽  
Vol 4 (3) ◽  
pp. 343-346 ◽  
Author(s):  
N Sakuragawa
Keyword(s):  
Gene Therapy ◽  
Epithelial Cells ◽  
Inborn Errors Of Metabolism ◽  
Inborn Errors ◽  
Amniotic Epithelial Cells ◽  
Human Amniotic Epithelial Cells

Immunostaining of Human Amniotic Epithelial Cells: Possible use as a Transgene Carrier in Gene Therapy for Inborn Errors of Metabolism

1995 ◽  
Vol 4 (3) ◽  
pp. 343-346 ◽  
Author(s):  
Norio Sakuragawa ◽  
Jun Tohyama ◽  
Hiroshi Yamamoto
Keyword(s):  
Gene Therapy ◽  
Inborn Errors Of Metabolism ◽  
Class Ii ◽  
Class I ◽  
Immunoperoxidase Staining ◽  
Galactosidase Activity ◽  
Flow Cytometry Analysis ◽  
Inborn Errors ◽  
Human Amniotic Epithelial Cells ◽  
Class Ii Antigen

Immunoreactivity of human cultured amniotic epithelial (AE) cells was investigated to evaluate the possible use of these cells as a transgene carrier in gene therapy for inborn errors of metabolism. AE cells were prepared and cultured by the methods described previously. Flow cytometry analysis revealed that these cells did not express any class II antigen at all on their surfaces. But the class I antigen was slightly expressed on their surfaces. Immunoperoxidase staining was slightly positive as to the class I antigen but not to the class II antigen at all. pSV-β-galactosidase was transfected into AE cells by means of electroporation, followed by staining of the cells with X-gal. Several cells in 60 mm dish expressed β-galactosidase activity. The possible gene transfer of β-galactosidase into cultured AE cells may suggest that these cells could be used as a transgene carrier in gene therapy for inborn errors of metabolism.

scholarly journals Human amniotic epithelial cells are promising transgene carriers for allogeneic cell transplantation into liver

2000 ◽  
Vol 45 (3) ◽  
pp. 171-176 ◽  
Author(s):  
N. Sakuragawa ◽  
S. Enosawa ◽  
T. Ishii ◽  
R. Thangavel ◽  
T. Tashiro ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Cell Transplantation ◽  
Allogeneic Cell ◽  
Amniotic Epithelial Cells ◽  
Human Amniotic Epithelial Cells

Expression of markers for both neuronal and glial cells in human amniotic epithelial cells

1996 ◽  
Vol 209 (1) ◽  
pp. 9-12 ◽  
Author(s):  
Norio Sakuragawa ◽  
Ramasamy Thangavel ◽  
Masashi Mizuguchi ◽  
Motoyuki Hirasawa ◽  
Isao Kamo
Keyword(s):  
Epithelial Cells ◽  
Glial Cells ◽  
Amniotic Epithelial Cells ◽  
Human Amniotic Epithelial Cells

scholarly journals Human Amniotic Epithelial Cells Possess Hepatocyte-like Characteristics and Functions

2004 ◽  
Vol 29 (3) ◽  
pp. 73-84 ◽  
Author(s):  
Seiji Takashima ◽  
Hirohiko Ise ◽  
Peng Zhao ◽  
Toshihiro Akaike ◽  
Toshio Nikaido
Keyword(s):  
Epithelial Cells ◽  
Amniotic Epithelial Cells ◽  
Human Amniotic Epithelial Cells

scholarly journals Functional Screening of TLRs in Human Amniotic Epithelial Cells

2011 ◽  
Vol 187 (5) ◽  
pp. 2766-2774 ◽  
Author(s):  
Claire Gillaux ◽  
Céline Méhats ◽  
Daniel Vaiman ◽  
Dominique Cabrol ◽  
Michelle Breuiller-Fouché
Keyword(s):  
Epithelial Cells ◽  
Functional Screening ◽  
Amniotic Epithelial Cells ◽  
Human Amniotic Epithelial Cells

scholarly journals Establishment and Characterization of Immortalized Human Amniotic Epithelial Cells

2013 ◽  
Vol 15 (1) ◽  
pp. 55-67 ◽  
Author(s):  
Kaixuan Zhou ◽  
Chika Koike ◽  
Toshiko Yoshida ◽  
Motonori Okabe ◽  
Moustafa Fathy ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Amniotic Epithelial Cells ◽  
Human Amniotic Epithelial Cells
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