Effects of 20-hydroxyecdysone on protein synthesis in the epithelial cell line from Chironomus tentans

1993 ◽  
Vol 23 (1) ◽  
pp. 159-164 ◽  
Author(s):  
Andreas Fretz ◽  
Andrea Lübbert ◽  
Ulrich Markmann-Mulisch ◽  
Margarethe Spindler-Barth ◽  
Klaus-Dieter Spindler
Keyword(s):  
Protein Synthesis ◽  
Epithelial Cell ◽  
Cell Line ◽  
Epithelial Cell Line ◽  
Chironomus Tentans

Action of Brassinosteroids on the Epithelial Cell Line from Chironomus tentans

1992 ◽  
Vol 47 (3-4) ◽  
pp. 280-284 ◽  
Author(s):  
Klaus-Dieter Spindler ◽  
Margarethe Spindler-Barth ◽  
Andreas Turberg ◽  
Günter Adam
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Epithelial Cell Line ◽  
Chironomus Tentans ◽  
Chitin Synthesis ◽  
Ecdysteroid Receptor ◽  
High Concentrations ◽  
Moulting Hormones ◽  
Ponasterone A

Abstract The two brassinosteroids, 22S′,23S′-homobrassinolide and 22S′,23S′-homocastasterone are weak competitors of the binding of [3H]ponasterone A to the intracellular ecdysteroid receptor from the epithelial cell line from Chironom us tentans. The relative affinities to the ecdysteroid receptor are 0.001 for both brassinosteroids as compared to 20-OH-ecdysone and 0.1 in comparison to ecdysone. Both substances exert morphological effects similar to those observed with 20-OH-ecdysone. Like moulting hormones both brassinosteroids inhibit chitin synthesis. However, these effects were observed only at rather high concentrations (10-5 to 10-4 m) which were cytotoxic for 22 S′,23 S′-homobrassinolide.

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