Regulation of Insulin-Like Growth Factor Binding Protein Synthesis and Secretion in a Bovine Epithelial Cell Line*

Endocrinology ◽  
1991 ◽  
Vol 129 (3) ◽  
pp. 1347-1354 ◽  
Author(s):  
W. S. COHICK ◽  
D. R. CLEMMONS
Keyword(s):  
Protein Synthesis ◽  
Growth Factor ◽  
Epithelial Cell ◽  
Cell Line ◽  
Binding Protein ◽  
Epithelial Cell Line ◽  
Insulin Like Growth Factor ◽  
Factor Binding

INSULIN AND VARIATION IN GLUCOSE LEVELS MODIFY THE SECRETION RATES OF THE GROWTH HORMONE-INDEPENDENT INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN-1 IN THE HUMAN HEPATOBLASTOMA CELL LINE HEP G2

1989 ◽  
Vol 123 (3) ◽  
pp. R17-R20 ◽  
Author(s):  
A.M. Cotterill ◽  
C.T. Cowell ◽  
M. Silink
Keyword(s):  
Growth Factor ◽  
Cell Line ◽  
Glucose Concentration ◽  
Binding Protein ◽  
Suitable Model ◽  
Insulin Like Growth Factor ◽  
Hep G2 ◽  
Factor Binding ◽  
Glucose Levels

ABSTRACT The plasma level of the GH-independent insulin-like growth factor binding-protein-1 (IGFBP-1) is regulated inversely by insulin. In this study the effect of insulin and changes in the glucose concentration on in-vitro IGFBP-1 secretion by the Hep G2 cell line was studied. Media from confluent cells in 12 replicates were collected for consecutive periods: initial control (20 h), study(6 h) and recovery (20 h). Insulin suppressed IGFBP-1 secretion maximally at 100 mU/1 (−32%) within 6 h. The secretion of IGFBP-1 was stimulated by a decrease in the glucose concentration in the medium, maximally (+25%) with a decrease from 24 to 6 mmol/l. Stimulation by varying glucose levels and suppression by insulin of IGFBP-1 secretion persisted on return to control conditions after the removal of physiological concentrations of glucose (4 - 12 mmol/l) and insulin (50 - 500 mU/1). The findings in the Hep G2 cell line that a variation in the physiological concentrations of glucose and insulin each independently regulate IGFBP-1 secretion suggest that this cell line may by a suitable model for further in-vitro studies of the regulation of secretion of IGFBP-1.

scholarly journals Antiproliferative Effects of Insulin-like Growth Factor-binding Protein-3 in Mesenchymal Chondrogenic Cell Line RCJ3.1C5.18

2000 ◽  
Vol 276 (8) ◽  
pp. 5533-5540 ◽  
Author(s):  
Anna Spagnoli ◽  
Vivian Hwa ◽  
William A. Horton ◽  
Gregory P. Lunstrum ◽  
Charles T. Roberts ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cell Line ◽  
Binding Protein ◽  
Insulin Like Growth Factor ◽  
Antiproliferative Effects ◽  
Factor Binding
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