Inhibition of Apical Membrane Enzyme Activities and Protein Synthesis by Gentamicin in a Kidney Epithelial Cell Line LLC-PK1.

Aminoglycoside-induced renal cell injury was investigated using the LLC-PK1 pig kidney epithelial cell line. The development of aminopeptidase and gamma-glutamyltransferase, marker enzymes for apical membranes, was inhibited in the LLC-PK1 cells cultured with gentamicin. The inhibitory effect of gentamicin on the enzyme activities was dose dependent and was related to its accumulation within the cells. The development of Na+ -dependent active transport of alpha-methyl-D-glucoside, a nonmetabolizable hexose, was also inhibited by treatment with gentamicin. Inhibitions in apical membrane enzyme activities and Na+ -dependent transport of alpha-methyl-D-glucoside were associated with the elevation of cytosolic free calcium, determined with a fluorescent indicator fura-2. The correlation between the alterations in apical membrane functions and the increase in cytosolic free calcium concentration was also supported by the study using the calcium ionophore A23187. The present data suggest that aminoglycoside-induced alterations in apical membranes of the LLC-PK1 cells are related to the increase in cytosolic free calcium levels.

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Enzyme activities and sodium-dependent active d-glucose transport in apical membrane vesicles isolated from kidney epithelial cell line (LLC-PK1)

Biochimica et Biophysica Acta (BBA) - Biomembranes ◽

10.1016/0005-2736(84)90340-7 ◽

1984 ◽

Vol 769 (2) ◽

pp. 514-518 ◽

Cited By ~ 21

Author(s):

Ken-Ichi Inui ◽

Hideyuki Saito ◽

Mikihisa Takano ◽

Tomonobu Okano ◽

Shikifumi Kitazawa ◽

...

Keyword(s):

Epithelial Cell ◽

Cell Line ◽

Enzyme Activities ◽

Apical Membrane ◽

Membrane Vesicles ◽

Epithelial Cell Line ◽

Sodium Dependent ◽

Kidney Epithelial Cell ◽

Kidney Epithelial Cell Line ◽

Apical Membrane Vesicles

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Expression of a differentiated transport function in apical membrane vesicles isolated from an established kidney epithelial cell line. Sodium electrochemical potential-mediated active sugar transport.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)34182-6 ◽

1982 ◽

Vol 257 (15) ◽

pp. 8680-8686

Author(s):

J E Lever

Keyword(s):

Epithelial Cell ◽

Cell Line ◽

Apical Membrane ◽

Sugar Transport ◽

Membrane Vesicles ◽

Epithelial Cell Line ◽

Transport Function ◽

Kidney Epithelial Cell ◽

Kidney Epithelial Cell Line ◽

Apical Membrane Vesicles

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Transport by the (Na+, K+) ATPase: Modulation by differentiation inducers and inhibition of protein synthesis in the MDCK kidney epithelial cell line

Journal of Cellular Physiology ◽

10.1002/jcp.1041230317 ◽

1985 ◽

Vol 123 (3) ◽

pp. 410-416 ◽

Cited By ~ 14

Author(s):

Brian G. Kennedy ◽

Julia E. Lever

Keyword(s):

Protein Synthesis ◽

Epithelial Cell ◽

Cell Line ◽

Epithelial Cell Line ◽

Inhibition Of Protein Synthesis ◽

Kidney Epithelial Cell ◽

Kidney Epithelial Cell Line ◽

Differentiation Inducers

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H+-gradient-dependent active transport of tetraethylammonium cation in apical-membrane vesicles isolated from kidney epithelial cell line LLC-PK1

Biochemical Journal ◽

10.1042/bj2270199 ◽

1985 ◽

Vol 227 (1) ◽

pp. 199-203 ◽

Cited By ~ 59

Author(s):

K Inui ◽

H Saito ◽

R Hori

Keyword(s):

Epithelial Cell ◽

Cell Line ◽

Apical Membrane ◽

Membrane Vesicles ◽

Epithelial Cell Line ◽

Overshoot Phenomenon ◽

Kidney Epithelial Cell ◽

Kidney Epithelial Cell Line ◽

Apical Membranes ◽

Apical Membrane Vesicles

Transport of [3H]tetraethylammonium (NEt4+), an organic cation, has been studied by using apical-membrane vesicles isolated from cultured kidney epithelial cell line LLC-PK1. The uptake of NEt4+ by apical-membrane vesicles was osmotically sensitive, time-dependent and saturable. The presence of an H+ gradient ([H+]i greater than [H+]o) induced a marked stimulation of NEt4+ uptake against its concentration gradient (overshoot phenomenon), and this concentrative uptake was inhibited by HgCl2. These results suggest that apical membranes isolated from the LLC-PK1 cells retain the transport characteristics of NEt4+ similar to those observed in renal brush-border membranes.

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