AROMATIC HYDROXYLATION OF PHENETHYLAMINE, AMPHETAMINE AND PHENTERMINE BY RAT LIVER MICRO-SOMES; A COMPARABLE STUDY

Abstracts ◽  
1977 ◽  
pp. 135
Author(s):  
J.A. Jonsson ◽  
B. Lindeke
Keyword(s):  
Rat Liver ◽  
Aromatic Hydroxylation

scholarly journals Cytochrome P450 Isozymes Involved in Aromatic Hydroxylation and Side-Chain N-Desisopropylation of Alprenolol in Rat Liver Microsomes.

1995 ◽  
Vol 18 (8) ◽  
pp. 1060-1065 ◽  
Author(s):  
Shizuo NARIMATSU ◽  
Masaya TACHIBANA ◽  
Yasuhiro MASUBUCHI ◽  
Susumu IMAOKA ◽  
Yoshihiko FUNAE ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Rat Liver ◽  
Liver Microsomes ◽  
Side Chain ◽  
Rat Liver Microsomes ◽  
Aromatic Hydroxylation ◽  
P450 Isozymes ◽  
Cytochrome P450 Isozymes

Reversible inhibition of aromatic hydroxylation of methamphetamine in rat liver microsomal preparations pretreated with methamphetamine

1988 ◽  
Vol 37 (8) ◽  
pp. 1433-1437 ◽  
Author(s):  
Toshinori Yamamoto ◽  
Ritsuko Takano ◽  
Toru Egashira ◽  
Yasumitu Yamanaka
Keyword(s):  
Rat Liver ◽  
Reversible Inhibition ◽  
Aromatic Hydroxylation

Aromatic hydroxylation of amphetamine with rat liver microsomes, perfused liver, and isolated hepatocytes

1978 ◽  
Vol 27 (21) ◽  
pp. 2525-2529 ◽  
Author(s):  
Ruth E. Billings ◽  
Patrick J. Murphy ◽  
Robert E. McMahon ◽  
James Ashmore
Keyword(s):  
Rat Liver ◽  
Liver Microsomes ◽  
Isolated Hepatocytes ◽  
Rat Liver Microsomes ◽  
Perfused Liver ◽  
Aromatic Hydroxylation

scholarly journals Contribution of cytochromes and proteins to the effect of ascorbic acid on artificial and microsomal hydroxylation systems containing oxygen and hydrogen peroxide

1978 ◽  
Vol 170 (3) ◽  
pp. 693-698 ◽  
Author(s):  
J Chrastil ◽  
J T Wilson
Keyword(s):  
Hydrogen Peroxide ◽  
Ascorbic Acid ◽  
Free Radical ◽  
Cytochrome C ◽  
Rat Liver ◽  
Oxidative Demethylation ◽  
Aromatic Hydroxylation ◽  
Microsomal Hydroxylation ◽  
Cytochrome P 450

Hydroxylation systems containing cytochromes, proteins and ascorbic acid were studied at physiological pH (7.4) under O2 or N2 with added H2O2. Proteins inhibited aromatic hydroxylation of p-nitrophenol or oxidative demethylation of ethylmorphine in ascorbic acid-containing systems incubated under O2, but strongly activated the systems containing H2O2. Cytochrome c and partially purified cytochrome P-450 from rat liver microsomal preparations activated the system in either O2 or H2O2. The systems needed ascorbic acid (or other enol structures) for activation. Cytochrome iron participated probably in the activation of O2, whereas cytochrome protein participated in a free radical activation of H2O2 (or of O2).

scholarly journals Ring hydroxylation of di-t-butylhydroxytoluene by rat liver microsomal preparations

1972 ◽  
Vol 128 (5) ◽  
pp. 1285-1291 ◽  
Author(s):  
Yon-Shong Shaw ◽  
Chiadao Chen
Keyword(s):  
Rat Liver ◽  
Compound I ◽  
Aromatic Hydroxylation ◽  
Compound Ii ◽  
Ring Hydroxylation

3,5-Di-t-butylhydroxytoluene (compound I) was converted into 4-hydroperoxy-4-methyl-2,6-di-t-butylcyclohexa-2,5-dienone (compound II), 4-hydroxy-4-methyl-2,6-di-t-butylcyclohexa-2,5-dienone (compound III) and 2,6-di-t-butyl-4-hydroxymethylphenol (compound IV) by rat liver microsomal preparations in the presence of NADPH and air. The oxidation of compound (I) by m-chloroperbenzoic acid also produced the same compounds. These results suggest that hydroperoxide can be an intermediate in aromatic hydroxylation and that biological oxygenations resemble per-acid reactions.

Initial Polymerization Sites Indicated During Mitochondrial Oxidation of Diaminobenzidine after Toluene Treatment

1977 ◽  
Vol 35 ◽  
pp. 408-409
Author(s):  
W. A. Shannon ◽  
M. A. Matlib
Keyword(s):  
Membrane Permeability ◽  
Cytochrome Oxidase ◽  
Rat Liver ◽  
Precise Definition ◽  
Cytochemical Localization ◽  
Oxidative Reaction ◽  
Mitochondrial Oxidation ◽  
Definition Of ◽  
Exogenous Substrates

Numerous studies have dealt with the cytochemical localization of cytochrome oxidase via cytochrome c. More recent studies have dealt with indicating initial foci of this reaction by altering incubation pH (1) or postosmication procedure (2,3). The following study is an attempt to locate such foci by altering membrane permeability. It is thought that such alterations within the limits of maintaining morphological integrity of the membranes will ease the entry of exogenous substrates resulting in a much quicker oxidation and subsequently a more precise definition of the oxidative reaction.The diaminobenzidine (DAB) method of Seligman et al. (4) was used. Minced pieces of rat liver were incubated for 1 hr following toluene treatment (5,6). Experimental variations consisted of incubating fixed or unfixed tissues treated with toluene and unfixed tissues treated with toluene and subsequently fixed.

Effects of 5-5'-Diphenyl-2-thiohydantoin (DPTH) and Thyroxine on the Ultrastructure and Chemical Composition of Rat Liver

1971 ◽  
Vol 29 ◽  
pp. 570-571
Author(s):  
E. A. Elfont ◽  
R. B. Tobin ◽  
D. G. Colton ◽  
M. A. Mehlman
Keyword(s):  
Chemical Composition ◽  
Rat Liver ◽  
Future Study ◽  
Mode Of Action ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Effective Inhibitor ◽  
Biochemical Effects ◽  
Glycerophosphate Dehydrogenase ◽  
Stimulation Of

Summary5,-5'-diphenyl-2-thiohydantoin (DPTH) is an effective inhibitor of thyroxine (T4) stimulation of α-glycerophosphate dehydrogenase in rat liver mitochondria. Because this finding indicated a possible tool for future study of the mode of action of thyroxine, the ultrastructural and biochemical effects of DPTH and/or thyroxine on rat liver mere investigated.Rats were fed either standard or DPTH (0.06%) diet for 30 days before T4 (250 ug/kg/day) was injected. Injection of T4 occurred daily for 10 days prior to sacrifice. After removal of the liver and kidneys, part of the tissue was frozen at -50°C for later biocheailcal analyses, while the rest was prefixed in buffered 3.5X glutaraldehyde (390 mOs) and post-fixed in buffered 1Z OsO4 (376 mOs). Tissues were embedded in Araldlte 502 and the sections examined in a Zeiss EM 9S.Hepatocytes from hyperthyroid rats (Fig. 2) demonstrated enlarged and more numerous mitochondria than those of controls (Fig. 1). Glycogen was almost totally absent from the cytoplasm of the T4-treated rats.

Effects of Steroids on Fetal Rat Liver

1969 ◽  
Vol 27 ◽  
pp. 350-351
Author(s):  
F. G. Zaki
Keyword(s):  
Liver Injury ◽  
Rat Liver ◽  
Fetal Liver ◽  
Dosage Level ◽  
Maternal Plasma ◽  
Methyl Prednisolone ◽  
Fetal Rat ◽  
Toxic Agents ◽  
Fetal Rat Liver ◽  
Neonatal Liver

Fetal and neonatal liver injury induced by agents circulating in maternal plasma, even though well recognized, its morphological manifestations are not yet established. As part of our studies of fetal and neonatal liver injury induced by maternal nutritional disorders, metabolic impairment and toxic agents, the effects of two anti-inflammatory steroids have been recently inves tigated.Triamcinolone and methyl prednisolone were injected each in a group of rats during pregnancy at a-dosage level of 2 mgm three times a week. Fetal liver was studied at 18 days of gestation. Litter size and weight markedly decreased than those of control rats. Stillbirths and resorption were of higher incidence in the triamcinolone group than in those given the prednisolone.

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