Initial Polymerization Sites Indicated During Mitochondrial Oxidation of Diaminobenzidine after Toluene Treatment

1977 ◽  
Vol 35 ◽  
pp. 408-409
Author(s):  
W. A. Shannon ◽  
M. A. Matlib
Keyword(s):  
Membrane Permeability ◽  
Cytochrome Oxidase ◽  
Rat Liver ◽  
Precise Definition ◽  
Cytochemical Localization ◽  
Oxidative Reaction ◽  
Mitochondrial Oxidation ◽  
Definition Of ◽  
Exogenous Substrates

Numerous studies have dealt with the cytochemical localization of cytochrome oxidase via cytochrome c. More recent studies have dealt with indicating initial foci of this reaction by altering incubation pH (1) or postosmication procedure (2,3). The following study is an attempt to locate such foci by altering membrane permeability. It is thought that such alterations within the limits of maintaining morphological integrity of the membranes will ease the entry of exogenous substrates resulting in a much quicker oxidation and subsequently a more precise definition of the oxidative reaction.The diaminobenzidine (DAB) method of Seligman et al. (4) was used. Minced pieces of rat liver were incubated for 1 hr following toluene treatment (5,6). Experimental variations consisted of incubating fixed or unfixed tissues treated with toluene and unfixed tissues treated with toluene and subsequently fixed.

scholarly journals The mitochondrial oxidation of quinol monophosphates

1970 ◽  
Vol 118 (5) ◽  
pp. 719-731
Author(s):  
J. M. Young
Keyword(s):  
Electron Transport ◽  
Cytochrome C ◽  
Oxygen Uptake ◽  
Cytochrome Oxidase ◽  
Rat Liver ◽  
Mode Of Action ◽  
Electron Transport Chain ◽  
Rapid Reduction ◽  
Transport Chain ◽  
Mitochondrial Oxidation

1. Mitochondria from ox heart and rat liver catalysed a slow cyanide-sensitive oxidation of 2,3-dimethylnaphthaquinol monophosphate, duroquinol monophosphate, menadiol 1-phosphate and menadiol 4-phosphate. 2. The release of Pi was concomitant with oxygen uptake. 3. The oxidation was somewhat stimulated by Ca2+ and Pi, and weakly inhibited by 2,4-dinitrophenol. 4. The quinol monophosphates effected a rapid reduction of free cytochrome c, and consequently addition of cytochrome c greatly increased the rate of the mitochondrial oxidation of 2,3-dimethylnaphthaquinol monophosphate. 5. This quinol phosphate interacts with the electron-transport chain at the level of cytochrome c. 6. Polylysine promoted an interaction between 2,3-dimethylnaphthaquinol monophosphate and cytochrome oxidase. Thus, although polylysine blocks mitochondrial oxidations via reduced cytochrome c, the oxidation of the quinol phosphate was strongly stimulated. 7. This stimulation was most effective in the most intact mitochondrial preparations and was inhibited by ADP and by Pi. 8. The implications of these results for factors limiting the rate of quinol phosphate oxidation, the mode of action of stimulators and the mechanism of Pi formation are discussed.

Authentic Culinary Tourism Experiences: The Perspectives of Locals

2020 ◽  
Author(s):  
Susan C. Graham
Keyword(s):  
Prince Edward Island ◽  
Precise Definition ◽  
Scholarly Work ◽  
Culinary Tourism ◽  
Work Related ◽  
Napa Valley ◽  
Definition Of

Culinary experiences have long been an important aspect of tourism. For many destinations, culinary offerings have become ubiquitous with the place – pasta in Italy, wine in the Loire- or Napa Valley, or curry in India. As tourists increasingly seek out authentic touristic experiences, including culinary experiences, the question arises regarding what constitutes an authentic culinary experience in a place. While authentic and authenticity are terms widely used in the tourism literature, a precise definition of what those terms mean and a method for identifying that which is authentic remains elusive. Research regarding authenticity in tourism suggests that locals occupy a ‘place of privilege’ with respect to determining the authenticity of a touristic experience because of their connection to and context in relation to the place. This paper examines the perspectives of Prince Edward Island (PEI) residents with respect to what constitutes an authentic culinary touristic experience in which visitors to Canada’s smallest province can partake and that provide those visitors with a glimpse of what life in PEI is or was really like, and provides a voice for an underrepresented group in the authenticity discourse. Results show that authentic culinary experiences transcend food, and encompass people, places, and experiences in ways that enrich touristic endeavours, and that locals understand and interpret authenticity in ways that both conform to and differ from existing scholarly work related to tourism authenticity, and span objective, existential, and constructive authenticity.

Two Theories of Effective Government

2017 ◽  
Author(s):  
Johannes Lindvall
Keyword(s):  
Political Science ◽  
Political Institutions ◽  
Public Policies ◽  
Methodological Approach ◽  
Decision Makers ◽  
Precise Definition ◽  
Policy Changes ◽  
Political Decision ◽  
Definition Of ◽  
The Relationship

This chapter introduces the problem of “reform capacity” (the ability of political decision-makers to adopt and implement policy changes that benefit society as a whole, by adjusting public policies to changing economic, social, and political circumstances). The chapter also reviews the long-standing discussion in political science about the relationship between political institutions and effective government. Furthermore, the chapter explains why the possibility of compensation matters greatly for the politics of reform; provides a precise definition of the concept of reform capacity; describes the book's general approach to this problem; and discusses the ethics of compensating losers from reform; and presents the book's methodological approach.

Clarifying and expanding the theory typology: A reply to readers’ comments

2021 ◽  
pp. 147059312110127
Author(s):  
Jörgen Sandberg ◽  
Mats Alvesson
Keyword(s):  
Social Science ◽  
Precise Definition ◽  
Definition Of

We all want to develop impactful and valuable theories, but what do we mean by ‘theory’? In this piece, we reply to the four readers’ comments on a recent paper in which we propose a theory typology that, in comparison to existing literature, offers a considerably broader, more pluralistic and, at the same time, more precise definition of the meaning(s) of ‘theory’ within academia. In their comments, the readers raise several insightful points that encourage us to further clarify the theory typology, as well as to expand on how it can be useful for researchers in marketing and social science more broadly.

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