A Restriction Enzyme from Hemophilus influenzae II. Base Sequence of the Recognition Site

1989 ◽  
pp. 464-480
Author(s):  
THOMAS J. KELLY ◽  
HAMILTON O. SMITH
Keyword(s):  
Restriction Enzyme ◽  
Recognition Site ◽  
Base Sequence ◽  
Hemophilus Influenzae

A Restriction enzyme from Hemophilus influenzae

1970 ◽  
Vol 51 (2) ◽  
pp. 379-391 ◽  
Author(s):  
Hamilton O. Smith ◽  
K.W. Welcox
Keyword(s):  
Restriction Enzyme ◽  
Hemophilus Influenzae

A rapid and efficient PCR-based mutagenesis method applicable to cell physiology study

2005 ◽  
Vol 288 (6) ◽  
pp. C1273-C1278 ◽  
Author(s):  
Jae-Kyun Ko ◽  
Jianjie Ma
Keyword(s):  
Molecular Biology ◽  
Protein Engineering ◽  
Restriction Enzyme ◽  
Cost Effective ◽  
Restriction Enzymes ◽  
Recognition Site ◽  
Cell Physiology ◽  
Multiple Site ◽  
Functional Studies ◽  
Engineering Studies

PCR-based mutagenesis is a cornerstone of molecular biology and protein engineering studies. Herein we describe a rapid and highly efficient mutagenesis method using type IIs restriction enzymes. A template gene is amplified into two separate PCR fragments using two pairs of anchor and mutagenic primers. Mutated sequences are located near the recognition site of a type IIs restriction enzyme. After digestion of two fragments with a type IIs enzyme, exposed cohesive ends that are complementary to each other are then ligated together to generate a mutated gene. We applied this method to introduce multiple site-directed mutations in EGFP and Bcl-2 family genes and observed perfect mutagenesis efficiency at the desired sites. This efficient and cost-effective mutagenesis method can be applied to a wide variety of structural and functional studies in cell physiology.

scholarly journals Recognition site of Escherichia coli B restriction enzyme on  XsB1 and simian virus 40 DNAs: An interrupted sequence

1978 ◽  
Vol 75 (5) ◽  
pp. 2271-2275 ◽  
Author(s):  
J. A. Lautenberger ◽  
N. C. Kan ◽  
D. Lackey ◽  
S. Linn ◽  
M. H. Edgell ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Restriction Enzyme ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Recognition Site ◽  
Escherichia Coli B

The recognition site of type II restriction enzyme BglI is interrupted

Gene ◽  
1980 ◽  
Vol 9 (3-4) ◽  
pp. 213-231 ◽  
Author(s):  
James A. Lautenberger ◽  
C. Thomas White ◽  
Nancy L. Haigwood ◽  
Marshall H. Edgell ◽  
Clyde A. Hutchison
Keyword(s):  
Restriction Enzyme ◽  
Recognition Site ◽  
Type Ii

A restriction enzyme from Hemophilus influenzae

1970 ◽  
Vol 51 (2) ◽  
pp. 393-409 ◽  
Author(s):  
Thomas J. Kelly ◽  
Hamilton O. Smith
Keyword(s):  
Restriction Enzyme ◽  
Hemophilus Influenzae
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