MUTATIONS IN BACILLUS SUBTILIS WHICH INFLUENCE THE ACTIVITY OF A PROMOTER RECOGNIZED BY A MINOR FORM OF RNA POLYMERASE (E-σB)

1990 ◽  
pp. 13-21 ◽  
Author(s):  
A.K. Benson ◽  
A. Stevenson ◽  
W.G. Haldenwang
Keyword(s):  
Bacillus Subtilis ◽  
Rna Polymerase ◽  
Minor Form ◽  
A Minor

scholarly journals Isolation of Bacillus subtilis mutants altered in expression of a gene transcribed in vitro by a minor form of RNA polymerase (E-sigma 37).

1985 ◽  
Vol 161 (2) ◽  
pp. 515-522 ◽  
Author(s):  
C L Truitt ◽  
G L Ray ◽  
J E Trempy ◽  
Z Da-Jian ◽  
W G Haldenwang
Keyword(s):  
Bacillus Subtilis ◽  
Rna Polymerase ◽  
Minor Form ◽  
A Minor

scholarly journals epr Is Transcribed from a ςD Promoter and Is Involved in Swarming of Bacillus subtilis

2002 ◽  
Vol 184 (2) ◽  
pp. 596-599 ◽  
Author(s):  
Madhulika Dixit ◽  
Charuta S. Murudkar ◽  
K. Krishnamurthy Rao
Keyword(s):  
Bacillus Subtilis ◽  
Rna Polymerase ◽  
Extracellular Protease ◽  
A Minor

ABSTRACT Epr is a minor extracellular protease secreted by Bacillus subtilis 168. In this study, we show that epr is transcribed by EςD, the RNA polymerase associated with transcription of genes involved in chemotaxis and motility. Disruption of epr abolished swarming of Bacillus subtilis, suggesting its involvement in motility.

scholarly journals The δ subunit and NTPase HelD institute a two-pronged mechanism for RNA polymerase recycling

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Hao-Hong Pei ◽  
Tarek Hilal ◽  
Zhuo A. Chen ◽  
Yong-Heng Huang ◽  
Yuan Gao ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Nucleic Acids ◽  
Rna Polymerase ◽  
Active Site ◽  
Genome Stability ◽  
Slow Growth ◽  
Coiled Coil ◽  
Main Channel ◽  
Dependent Manner ◽  
Secondary Channel

AbstractCellular RNA polymerases (RNAPs) can become trapped on DNA or RNA, threatening genome stability and limiting free enzyme pools, but how RNAP recycling into active states is achieved remains elusive. In Bacillus subtilis, the RNAP δ subunit and NTPase HelD have been implicated in RNAP recycling. We structurally analyzed Bacillus subtilis RNAP-δ-HelD complexes. HelD has two long arms: a Gre cleavage factor-like coiled-coil inserts deep into the RNAP secondary channel, dismantling the active site and displacing RNA, while a unique helical protrusion inserts into the main channel, prying the β and β′ subunits apart and, aided by δ, dislodging DNA. RNAP is recycled when, after releasing trapped nucleic acids, HelD dissociates from the enzyme in an ATP-dependent manner. HelD abundance during slow growth and a dimeric (RNAP-δ-HelD)2 structure that resembles hibernating eukaryotic RNAP I suggest that HelD might also modulate active enzyme pools in response to cellular cues.

scholarly journals DNA-dependent RNA polymerase from vegetative cells and from spores of Bacillus subtilis

FEBS Letters ◽  
1971 ◽  
Vol 13 (5) ◽  
pp. 269-274 ◽  
Author(s):  
J.C.C. Maia ◽  
P. Kerjan ◽  
J. Szulmajster
Keyword(s):  
Bacillus Subtilis ◽  
Rna Polymerase ◽  
Vegetative Cells
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