NOVEL LOW MOLECULAR WEIGHT MITOGENIC FACTOR FROM BRL 3A RAT LIVER CELL CONDITIONED MEDIUM

1981 ◽  
pp. 549
Author(s):  
Daniel S. Straus ◽  
Donald L. Coppock
Keyword(s):  
Molecular Weight ◽  
Conditioned Medium ◽  
Rat Liver ◽  
Liver Cell ◽  
Low Molecular Weight ◽  
Mitogenic Factor ◽  
Cell Conditioned Medium

scholarly journals Protective effects of low-molecular-weight components of adipose stem cell-derived conditioned medium on dry eye syndrome in mice

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yuan-Chieh Lee ◽  
Li-Yi Sun ◽  
Jia-Rong Zhang
Keyword(s):  
Molecular Weight ◽  
Stem Cell ◽  
Conditioned Medium ◽  
Dry Eye ◽  
Goblet Cells ◽  
Low Molecular Weight ◽  
Protective Effects ◽  
Corneal Epithelial ◽  
Adipose Derived Stem Cell ◽  
Cell Conditioned Medium

AbstractThe present study demonstrated the protective effects of low-molecular-weight adipose-derived stem cell-conditioned medium (LADSC-CM) in a mouse model of dry eye syndrome. Mice subjected to desiccating stress and benzalkonium chloride had decreased tear secretion, impaired corneal epithelial tight junction with microvilli, and decreased conjunctival goblet cells. Topical application of adipose-derived stem cell-conditioned medium (ADSC-CM) stimulated lacrimal tear secretion, preserved tight junction and microvilli of the corneal epithelium, and increased the density of goblet cells and MUC16 expression in the conjunctiva. The low-molecular-weight fractions (< 10 kDa and < 3 kDa) of ADSC-CM (LADSC-CM) provided better protections than the > 10 kDa or > 3 kDa fractions of ADSC-CM. In the in vitro study, desiccation for 10 min or hyperosmolarity (490 osmols) for 24 h caused decreased viability of human corneal epithelial cells, which were reversed by LADSC-CM. The active ingredients in the LADSC-CM were lipophobic and stable after heating and lyophilization. Our study demonstrated that LADSC-CM had beneficial effects on experimental dry eye. It is worthy of further exploration for the active ingredient(s) and the mechanism.

Sign in / Sign up

Export Citation Format

Share Document