Phage Display Technology for Selection of Antibody Fragments

2018 ◽  
pp. 67-88
Author(s):  
Daniela Teixeira ◽  
Maria Gonzalez-Pajuelo
Keyword(s):  
Phage Display ◽  
Antibody Fragments ◽  
Phage Display Technology ◽  
Display Technology ◽  
Selection Of

A novel phage display vector for selection of target-specific peptides

2020 ◽  
Vol 33 ◽  
Author(s):  
Alex Chang ◽  
Joey P Ting ◽  
Alfonso Espada ◽  
Howard Broughton ◽  
Manuel Molina-Martin ◽  
...  
Keyword(s):  
Amino Acids ◽  
Fusion Protein ◽  
Phage Display ◽  
Copy Number ◽  
Wild Type ◽  
Phage Display Technology ◽  
Successful Isolation ◽  
Display Technology ◽  
Therapeutic Peptides ◽  
Selection Of

Abstract Intrinsic low display level of polypeptides on phage is a fundamental and limiting hurdle in successful isolation of target-specific binders by phage display technology. To circumvent this challenge, we optimized the copy number of peptides displayed on the phage surface using type 33 phage vector. We randomized the first 67 amino acids of the wild type PIII to identify mutants that would result in its reduced expression. Consequently, the display level was improved by 30-fold due to higher incorporation of the synthetic PIII–peptide fusion protein on the phage surface. Utilization of this novel phage vector should provide a solid basis for the discovery of therapeutic peptides.

scholarly journals Selection of Linkers for a Catalytic Single-chain Antibody Using Phage Display Technology

1996 ◽  
Vol 271 (26) ◽  
pp. 15682-15686 ◽  
Author(s):  
Ying Tang ◽  
Ning Jiang ◽  
Cushrow Parakh ◽  
Donald Hilvert
Keyword(s):  
Phage Display ◽  
Single Chain ◽  
Single Chain Antibody ◽  
Phage Display Technology ◽  
Display Technology ◽  
Selection Of

scholarly journals Use of phage display technology for the selection of monoclonal antibodies in allergen research and diagnostics

2014 ◽  
Vol 4 (S2) ◽  
Author(s):  
Marjolein Vandekerckhove ◽  
Bart Van Droogenbroeck ◽  
Marc Deloose ◽  
Hilde Lapeere ◽  
Gevaert Philippe
Keyword(s):  
Monoclonal Antibodies ◽  
Phage Display ◽  
Phage Display Technology ◽  
Display Technology ◽  
Selection Of

Selection of Recombinant Antibodies by Phage Display Technology and Application for Detection of Allergenic Brazil Nut (Bertholletia excelsa) in Processed Foods

2013 ◽  
Vol 61 (43) ◽  
pp. 10310-10319 ◽  
Author(s):  
Silvia de la Cruz ◽  
Inés María López-Calleja ◽  
Marcos Alcocer ◽  
Isabel González ◽  
Rosario Martín ◽  
...  
Keyword(s):  
Phage Display ◽  
Recombinant Antibodies ◽  
Processed Foods ◽  
Brazil Nut ◽  
Phage Display Technology ◽  
Bertholletia Excelsa ◽  
Display Technology ◽  
Selection Of
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