Selection of Recombinant Antibodies by Phage Display Technology and Application for Detection of Allergenic Brazil Nut (Bertholletia excelsa) in Processed Foods

2013 ◽  
Vol 61 (43) ◽  
pp. 10310-10319 ◽  
Author(s):  
Silvia de la Cruz ◽  
Inés María López-Calleja ◽  
Marcos Alcocer ◽  
Isabel González ◽  
Rosario Martín ◽  
...  
Keyword(s):  
Phage Display ◽  
Recombinant Antibodies ◽  
Processed Foods ◽  
Brazil Nut ◽  
Phage Display Technology ◽  
Bertholletia Excelsa ◽  
Display Technology ◽  
Selection Of

A novel phage display vector for selection of target-specific peptides

2020 ◽  
Vol 33 ◽  
Author(s):  
Alex Chang ◽  
Joey P Ting ◽  
Alfonso Espada ◽  
Howard Broughton ◽  
Manuel Molina-Martin ◽  
...  
Keyword(s):  
Amino Acids ◽  
Fusion Protein ◽  
Phage Display ◽  
Copy Number ◽  
Wild Type ◽  
Phage Display Technology ◽  
Successful Isolation ◽  
Display Technology ◽  
Therapeutic Peptides ◽  
Selection Of

Abstract Intrinsic low display level of polypeptides on phage is a fundamental and limiting hurdle in successful isolation of target-specific binders by phage display technology. To circumvent this challenge, we optimized the copy number of peptides displayed on the phage surface using type 33 phage vector. We randomized the first 67 amino acids of the wild type PIII to identify mutants that would result in its reduced expression. Consequently, the display level was improved by 30-fold due to higher incorporation of the synthetic PIII–peptide fusion protein on the phage surface. Utilization of this novel phage vector should provide a solid basis for the discovery of therapeutic peptides.

scholarly journals Selection of Linkers for a Catalytic Single-chain Antibody Using Phage Display Technology

1996 ◽  
Vol 271 (26) ◽  
pp. 15682-15686 ◽  
Author(s):  
Ying Tang ◽  
Ning Jiang ◽  
Cushrow Parakh ◽  
Donald Hilvert
Keyword(s):  
Phage Display ◽  
Single Chain ◽  
Single Chain Antibody ◽  
Phage Display Technology ◽  
Display Technology ◽  
Selection Of

scholarly journals Mimotopes for Mycotoxins Diagnosis Based on Random Peptides or Recombinant Antibodies from Phage Library

Molecules ◽  
2021 ◽  
Vol 26 (24) ◽  
pp. 7652
Author(s):  
Wei Sun ◽  
Yan Zhang ◽  
Zhigang Ju
Keyword(s):  
Phage Display ◽  
Recombinant Antibody ◽  
Recombinant Antibodies ◽  
Phage Display Library ◽  
Ease Of Use ◽  
Phage Library ◽  
Phage Display Technology ◽  
Random Peptide ◽  
Display Technology

Mycotoxins, the small size secondary metabolites of fungi, have posed a threat to the safety of medicine, food and public health. Therefore, it is essential to create sensitive and effective determination of mycotoxins. Based on the special affinity between antibody and antigen, immunoassay has been proved to be a powerful technology for the detection of small analytes. However, the tedious preparation and instability of conventional antibodies restrict its application on easy and fast mycotoxins detection. By virtue of simplicity, ease of use, and lower cost, phage display library provides novel choices for antibodies or hapten conjugates, and lead random peptide or recombinant antibody to becoming the promising and environmental friendly immune-reagents in the next generation of immunoassays. This review briefly describes the latest developments on mycotoxins detection using M13 phage display, mainly focusing on the recent applications of phage display technology employed in mycotoxins detection, including the introduction of phage and phage display, the types of phage displayed peptide/recombinant antibody library, random peptides/recombinant antibodies-based immunoassays, as well as simultaneous determination of multiple mycotoxins.

scholarly journals Use of phage display technology for the selection of monoclonal antibodies in allergen research and diagnostics

2014 ◽  
Vol 4 (S2) ◽  
Author(s):  
Marjolein Vandekerckhove ◽  
Bart Van Droogenbroeck ◽  
Marc Deloose ◽  
Hilde Lapeere ◽  
Gevaert Philippe
Keyword(s):  
Monoclonal Antibodies ◽  
Phage Display ◽  
Phage Display Technology ◽  
Display Technology ◽  
Selection Of

scholarly journals generation of hapten-specific recombinant antibodies: antibody phage display technology: a review

2012 ◽  
Vol 50 (No. 6) ◽  
pp. 231-252 ◽  
Author(s):  
J. Brichta ◽  
M. Hnilova ◽  
T. Viskovic
Keyword(s):  
Phage Display ◽  
Recombinant Dna ◽  
Recombinant Antibodies ◽  
Laboratory Animals ◽  
Automatic Process ◽  
Phage Display Technology ◽  
Current Trends ◽  
Antibody Phage ◽  
Antibody Phage Display ◽  
Display Technology

Production of antibodies has been revolutionized by the development of modern molecular biology methods for the expression of recombinant DNA. Phage display technology represents one of the most powerful tools for production and selection of recombinant antibodies and has been recognized as a valuable alternative way for the preparation of antibodies of a desired specificity. In comparison to poly- and monoclonal antibodies, recombinant antibodies using the phage display technology can be prepared faster, in more automatic process and with reduced consumption of laboratory animals. This review summarizes current trends of phage display technology with focus on the generation of hapten-specific recombinant antibodies and gives the examples of successful applications of phage display in the environmental analysis of low molecular weight compound.

scholarly journals Application of Recombinant Human scFv Antibody as a Powerful Tool to Monitor Nitrogen Fixing Biofertilizer in Rice and Legume

2021 ◽  
Author(s):  
K. K. Khaing ◽  
K. Rangnoi ◽  
H. Michlits ◽  
N. Boonkerd ◽  
N. Teaumroong ◽  
...  
Keyword(s):  
Phage Display ◽  
Recombinant Antibodies ◽  
Scfv Antibody ◽  
Nitrogen Fixing ◽  
Phage Display Technology ◽  
Human Scfv ◽  
Display Technology

Human scFv antibody generated from phage display technology was successfully used for the generation of specific recombinant antibodies: yiN92-1e10 and yiDOA9-162 for the detection of Bradyrhizobium strains SUTN9-2 and DOA9, respectively.

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