Polymerase Chain Reaction and Other Nucleic Acid Amplification Technology

2011 ◽  
pp. 1271-1281 ◽  
Author(s):  
Frederick S. Nolte ◽  
Charles E. Hill
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
Nucleic Acid Amplification ◽  
Chain Reaction ◽  
Nucleic Acid Amplification Technology ◽  
Polymerase Chain

Ribonuclease H-cleavable and recombinase-quenching fluorescent probes for the real-time detection of strand invasion based amplification

2019 ◽  
Vol 11 (43) ◽  
pp. 5568-5576
Author(s):  
Sonja Elf ◽  
Kevin E. Eboigbodin
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
Real Time ◽  
Fluorescent Probes ◽  
Nucleic Acid Amplification ◽  
Ribonuclease H ◽  
Chain Reaction ◽  
Amplification Method ◽  
Polymerase Chain ◽  
Strand Invasion

SIBA is an established nucleic acid amplification method that is used as an alternative to polymerase chain reaction (PCR).

scholarly journals La PCR e le sue varianti

2008 ◽  
Keyword(s):  
Polymerase Chain Reaction ◽  
Genetic Polymorphism ◽  
Nucleic Acid ◽  
Quantitative Pcr ◽  
Nucleic Acid Amplification ◽  
Genetic Identification ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Reference Tool

The book "La PCR e le sue varianti" is designed as a reference tool for those whose laboratory activities deal with methods based on nucleic acid amplification. The text provides the theoretical bases of the polymerase chain reaction (PCR) and its variants (e.g. RT-PCR, quantitative PCR, isothermic PCR) in a rapid and concise manner and describes the principal applications used for genetic identification and the study of genetic polymorphism, in the form of a protocol that can be easily consulted by the users.

scholarly journals The discovery of Bungowannah virus – an example of the need for conventional and new technologies

2009 ◽  
Vol 30 (4) ◽  
pp. 138
Author(s):  
Peter D Kirkland
Keyword(s):  
Monoclonal Antibody ◽  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
New Technologies ◽  
Nucleic Acid Amplification ◽  
Chain Reaction ◽  
Agar Gel ◽  
Human Illness ◽  
Polymerase Chain ◽  
Flying Fox

A novel disease in pigs and another new virus ? so where is the flying fox connection? This was one of the first questions that many observers asked from the sidelines. In this instance there was no known connection with flying foxes, no suggestion of human illness but, as the investigation unravelled, a probable cause was identified ? an apparently new virus that had close connections to an important pig pathogen that is exotic to Australia. This virus was, however, so genetically different from its relatives that pan reactive polymerase chain reaction-based assays would not detect it. It was so different antigenically that pan reactive monoclonal antibody panels would react with it. Nevertheless, a combination of simple agar gel immunodiffusion tests for antibodies and sophisticated nucleic acid amplification and sequencing methods proved to be the keys to the recognition of this virus.

Osteoarticular infection in children

2021 ◽  
Vol 103-B (3) ◽  
pp. 578-583
Author(s):  
Benoit Coulin ◽  
Giacomo Demarco ◽  
Vanessa Spyropoulou ◽  
Celine Juchler ◽  
Tanguy Vendeuvre ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
Epidemiological Data ◽  
Paediatric Population ◽  
Nucleic Acid Amplification ◽  
Chain Reaction ◽  
Kingella Kingae ◽  
Osteoarticular Infection ◽  
Osteoarticular Infections ◽  
Polymerase Chain

Aims We aimed to describe the epidemiological, biological, and bacteriological characteristics of osteoarticular infections (OAIs) caused by Kingella kingae. Methods The medical charts of all children presenting with OAIs to our institution over a 13-year period (January 2007 to December 2019) were reviewed. Among these patients, we extracted those which presented an OAI caused by K. kingae and their epidemiological data, biological results, and bacteriological aetiologies were assessed. Results K. kingae was the main reported microorganism in our paediatric population, being responsible for 48.7% of OAIs confirmed bacteriologically. K. kingae affects primarily children aged between six months and 48 months. The highest prevalence of OAI caused by K. kingae was between seven months and 24 months old. After the patients were 27 months old, its incidence decreased significantly. The incidence though of infection throughout the year showed no significant differences. Three-quarters of patients with an OAI caused by K. kingae were afebrile at hospital admission, 11% had elevated WBCs, and 61.2% had abnormal CRPs, whereas the ESR was increased in 75%, constituting the most significant predictor of an OAI. On MRI, we noted 53% of arthritis affecting mostly the knee and 31% of osteomyelitis located primarily in the foot. Conclusion K. kingae should be recognized currently as the primary pathogen causing OAI in children younger than 48 months old. Diagnosis of an OAI caused by K. kingae is not always obvious, since this infection may occur with a mild-to-moderate clinical and biological inflammatory response. Extensive use of nucleic acid amplification assays improved the detection of fastidious pathogens and has increased the observed incidence of OAI, especially in children aged between six months and 48 months. We propose the incorporation of polymerase chain reaction assays into modern diagnostic algorithms for OAIs to better identify the bacteriological aetiology of OAIs. Cite this article: Bone Joint J 2021;103-B(3):578–583.

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