Ribonuclease H-cleavable and recombinase-quenching fluorescent probes for the real-time detection of strand invasion based amplification

2019 ◽  
Vol 11 (43) ◽  
pp. 5568-5576
Author(s):  
Sonja Elf ◽  
Kevin E. Eboigbodin
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
Real Time ◽  
Fluorescent Probes ◽  
Nucleic Acid Amplification ◽  
Ribonuclease H ◽  
Chain Reaction ◽  
Amplification Method ◽  
Polymerase Chain ◽  
Strand Invasion

SIBA is an established nucleic acid amplification method that is used as an alternative to polymerase chain reaction (PCR).

scholarly journals KOMPARASI ANTARA POLYMERASE CHAIN REACTION (PCR) DAN LOOPMEDIATED ISOTHERMAL AMPLIFICATION (LAMP) DALAM DIAGNOSIS MOLEKULER

2016 ◽  
Vol 3 (2) ◽  
pp. 145
Author(s):  
Anggun Feranisa
Keyword(s):  
Infectious Disease ◽  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
Infectious Diseases ◽  
Diagnostic Method ◽  
Nucleic Acid Amplification ◽  
Molecular Diagnostic ◽  
Chain Reaction ◽  
Amplification Method ◽  
Polymerase Chain

Background: Molecular diagnostic is an emerging diagnostic method inpersonalized medicine/dentistry era. Usually, it uses nucleic acid amplificationmethod to detect various diseases. PCR is conventional nucleic acid amplification method. However, due to an urgency in infectious diseases’ diagnotic method, scientists developed LAMP as new nucleic acid amplification method.Discussion: There are various experiments used to develop LAMP as infectious diseases diagnostic method compared to PCR. The results are LAMP more sensitive, specific, rapid, and inexpensive than PCR.Conclusion: Both PCR and LAMP can be used as molecular diagnostic tools.LAMP prefer to used as infectious disease diagnostic method in poor anddeveloping countries.

scholarly journals Optimization of Real-time Reverse Transcriptase Polymerase Chain Reaction for Detection of Dengue Virus

2020 ◽  
pp. 1-7
Author(s):  
Kaunara A. Azizi ◽  
Arnold J. Ndaro ◽  
Athanasia Maro ◽  
Adonira Saro ◽  
Reginald A. Kavishe
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
Dengue Virus ◽  
Reverse Transcriptase ◽  
Real Time ◽  
Acid Extraction ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Reliable Technique ◽  
Polymerase Chain

Aims: This study was set to optimize conditions for real time reverse transcriptase polymerase chain reaction (RT-PCR) for detection of dengue virus by using rapid and simple nucleic acid extraction method. Methodology: One step and two step real time RT-PCR were evaluated in different PCR thermocyclers. Extraction of viral RNA was done by using a simple boom method. Results: The real time RT-PCR technique was successfully optimized using simple and rapid method for purification of nucleic acid, ‘boom method’. The technique works better when performed in a two-step procedure and can works well with all range of real time PCR machines. The optimized real time RT-PCR used in the present study is a valuable and reliable technique for routine diagnosis of dengue. Further investigation on the cost effectiveness in adopting this technique for routine screening and monitoring of the dengue infection should be done.

scholarly journals La PCR e le sue varianti

2008 ◽  
Keyword(s):  
Polymerase Chain Reaction ◽  
Genetic Polymorphism ◽  
Nucleic Acid ◽  
Quantitative Pcr ◽  
Nucleic Acid Amplification ◽  
Genetic Identification ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Reference Tool

The book "La PCR e le sue varianti" is designed as a reference tool for those whose laboratory activities deal with methods based on nucleic acid amplification. The text provides the theoretical bases of the polymerase chain reaction (PCR) and its variants (e.g. RT-PCR, quantitative PCR, isothermic PCR) in a rapid and concise manner and describes the principal applications used for genetic identification and the study of genetic polymorphism, in the form of a protocol that can be easily consulted by the users.

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