scholarly journals Relative analytical sensitivity of donor nucleic acid amplification technology screening and diagnostic real-time polymerase chain reaction assays for detection of Zika virus RNA

Transfusion ◽  
2017 ◽  
Vol 57 (3pt2) ◽  
pp. 734-747 ◽  
Author(s):  
Mars Stone ◽  
Marion C. Lanteri ◽  
Sonia Bakkour ◽  
Xutao Deng ◽  
Susan A. Galel ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
Real Time ◽  
Zika Virus ◽  
Nucleic Acid Amplification ◽  
Analytical Sensitivity ◽  
Chain Reaction ◽  
Virus Rna ◽  
Nucleic Acid Amplification Technology ◽  
Polymerase Chain

Ribonuclease H-cleavable and recombinase-quenching fluorescent probes for the real-time detection of strand invasion based amplification

2019 ◽  
Vol 11 (43) ◽  
pp. 5568-5576
Author(s):  
Sonja Elf ◽  
Kevin E. Eboigbodin
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
Real Time ◽  
Fluorescent Probes ◽  
Nucleic Acid Amplification ◽  
Ribonuclease H ◽  
Chain Reaction ◽  
Amplification Method ◽  
Polymerase Chain ◽  
Strand Invasion

SIBA is an established nucleic acid amplification method that is used as an alternative to polymerase chain reaction (PCR).

scholarly journals Zika virus RNA polymerase chain reaction on the utility channel of a commercial nucleic acid testing system

Transfusion ◽  
2018 ◽  
Vol 58 (3) ◽  
pp. 641-648 ◽  
Author(s):  
Mohamed Boujnan ◽  
Ashley J. Duits ◽  
Marco H.G.M. Koppelman
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
Rna Polymerase ◽  
Zika Virus ◽  
Testing System ◽  
Nucleic Acid Testing ◽  
Chain Reaction ◽  
Virus Rna ◽  
Polymerase Chain

scholarly journals Diagnostic Validation of the RealStar® Zika Virus Reverse Transcription Polymerase Chain Reaction Kit for Detection of Zika Virus RNA in Urine and Serum Specimens

2017 ◽  
Vol 97 (4) ◽  
pp. 1070-1071 ◽  
Author(s):  
Stephan Ölschläger ◽  
Dominique Rousset ◽  
Mirdad Kazanji ◽  
Mareen Zaruba ◽  
Antoine Enfissi
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Zika Virus ◽  
Chain Reaction ◽  
Virus Rna ◽  
Polymerase Chain ◽  
Diagnostic Validation ◽  
Urine And Serum

scholarly journals Overestimation and underestimation of hepatitis C virus RNA levels in a widely used real-time polymerase chain reaction-based method

Hepatology ◽  
2007 ◽  
Vol 46 (1) ◽  
pp. 22-31 ◽  
Author(s):  
Stéphane Chevaliez ◽  
Magali Bouvier-Alias ◽  
Rozenn Brillet ◽  
Jean-Michel Pawlotsky
Keyword(s):  
Polymerase Chain Reaction ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Real Time ◽  
Hepatitis C Virus Rna ◽  
Chain Reaction ◽  
Virus Rna ◽  
Polymerase Chain ◽  
Rna Levels

scholarly journals Evaluation of Amplification Targets for the Specific Detection ofBordetella pertussisUsing Real-Time Polymerase Chain Reaction

2014 ◽  
Vol 25 (4) ◽  
pp. 217-221 ◽  
Author(s):  
Mohammad Rubayet Hasan ◽  
Rusung Tan ◽  
Ghada N Al-Rawahi ◽  
Eva Thomas ◽  
Peter Tilley
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Target Genes ◽  
Reference Panel ◽  
Superior Performance ◽  
Clinical Samples ◽  
Analytical Sensitivity ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Polymerase Chain

BACKGROUND:Bordetella pertussisinfections continue to be a major public health challenge in Canada. Polymerase chain reaction (PCR) assays to detectB pertussisare typically based on the multicopy insertion sequence IS481, which offers high sensitivity but lacks species specificity.METHODS: A novelB pertussisreal-time PCR assay based on the porin gene was tested in parallel with several previously published assays that target genes such as IS481,ptx-promoter, pertactin and a putative thialase. The assays were evaluated using a reference panel of common respiratory bacteria including differentBordetellaspecies and 107 clinical nasopharyngeal specimens. Discrepant results were confirmed by sequencing the PCR products.RESULTS: Analytical sensitivity was highest for the assay targeting the IS481element; however, the assay lacked specificity forB pertussisin the reference panel and in the clinical samples. False-positive results were also observed with assays targeting theptx-promoter and pertactin genes. A PCR assay based on the thialase gene was highly specific but failed to detect all reference strains ofB pertussis. However, a novel assay targeting the porin gene demonstrated high specificity forB pertussisboth in the reference panel and in clinical samples and, based on sequence-confirmed results, correctly predicted allB pertussis-positive cases in clinical samples. According to Probit regression analysis, the 95% detection limit of the new assay was 4 colony forming units/reaction.CONCLUSION: A novel porin assay forB pertussisdemonstrated superior performance and may be useful for improved molecular detection ofB pertussisin clinical specimens.

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