DISTRIBUTION OF ACID PHOSPHATASE IN LIVER TISSUE OF DEXTRAN-INJECTED MICE AS OBSERVED WITH THE ELECTRON MICROSCOPE

1962 ◽  
pp. VV-12
Author(s):  
W.Th. Daems
Keyword(s):  
Electron Microscope ◽  
Acid Phosphatase ◽  
Liver Tissue

Prefertilization ovule development in Capsella: the dyad, tetrad, developing megaspore, and two-nucleate gametophyte

1986 ◽  
Vol 64 (4) ◽  
pp. 875-884 ◽  
Author(s):  
Patricia Schulz ◽  
William A. Jensen
Keyword(s):  
Cell Wall ◽  
Electron Microscope ◽  
Acid Phosphatase ◽  
De Novo ◽  
Periodic Acid ◽  
Aniline Blue ◽  
Ovule Development ◽  
Periodic Acid Schiff ◽  
Fluorescent Material ◽  
Autophagic Vacuoles

Ovules of Capsella bursa-pastoris at the dyad and tetrad stages of meiosis and at the megaspore and two-nucleate stages of the gametophyte were studied with the electron microscope. The cells of the dyad and tetrad are separated by aniline blue fluorescent cross walls and receive all types of organelles and autophagic vacuoles that were present in the meiocyte. Autophagic vacuoles enclose ribosomes and organelles and show reaction product for acid phosphatase. Autophagic vacuoles and some plastids are absorbed into the enlarging vacuoles of the growing megaspore. Other plastids appear to survive meiosis and there is no evidence for their de novo origin. Some mitochondria appear to degenerate in the enlarging megaspore but others look healthy and there is no evidence for the de novo origin of mitochondria. The nucleolus of the developing megaspore becomes very large and the cytoplasm is extremely dense with ribosomes. The cell wall is thickened by an electron-translucent, periodic acid – Schiff negative, aniline blue fluorescent material and contains plasmodesmata that link the megaspore with the nucellus. The plasmalemma of the growing megaspore produces microvilluslike extensions into this wall that disappear with the formation of the two-nucleate gametophyte. Plasmodesmata disappear from the cell wall at the four-nucleate stage.

scholarly journals DEVELOPMENT OF AN IMPROVED MEDIUM FOR THE ISOLATION OF LIVER MITOCHONDRIA

1956 ◽  
Vol 2 (5) ◽  
pp. 609-624 ◽  
Author(s):  
M. S. C. Birbeck ◽  
E. Reid
Keyword(s):  
Adverse Effect ◽  
Molecular Weight ◽  
Electron Microscope ◽  
Acid Phosphatase ◽  
Mitochondrial Fraction ◽  
Liver Mitochondria ◽  
High Concentration ◽  
Mitochondrial Fractions ◽  
Sucrose Medium ◽  
Optimal Medium

In view of the unsatisfactory appearance, under the electron microscope, of liver mitochondria isolated in isotonic sucrose medium, alternative media have been examined. It was found to be advantageous to replace sucrose by raffinose, and to add levan or, preferably, dextran, together with heparin in suitable concentration. With the optimal medium, the constituents of which are raffinose, versene (optional), dextran of high molecular weight, heparin, and AMP (optional), most of the mitochondria in the osmium-fixed pellet are apparently intact, and show the membranes characteristic of mitochondria as seen in cell sections. The optimal medium has no adverse effect on the activity of the several tissue enzymes which have been studied, except that Mg++-activated ATPase is partially inhibited if the medium is present in high concentration in the assay system. Mitochondrial fractions isolated in the new medium have, in common with sucrose fractions, appreciable "free" ATPase activity, this activity being evidently a poor criterion of mitochondrial integrity. Use of the new medium does not decrease the proportion of cytoplasmic ATPase which fails to sediment with the mitochondria, but does give a mitochondrial fraction low in RNA and in acid phosphatase activity and little contaminated with microsomal material. Particles tentatively identified as "lysosomes" have been seen in certain sections.

scholarly journals DIAZOPHTHALOCYANINS AS REAGENTS FOR FINE STRUCTURAL CYTOCHEMISTRY

1965 ◽  
Vol 25 (1) ◽  
pp. 23-41 ◽  
Author(s):  
Lois Withrow Tice ◽  
Russell J. Barrnett
Keyword(s):  
Electron Microscope ◽  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Alcian Blue ◽  
Azo Dyes ◽  
Acid Phosphatase Activity ◽  
Fixed Material

This paper reports the synthesis of 14 diazophthalocyanins containing Mg, Cu, or Pb as the chelated metal. To assess the usefulness of these compounds for fine structural cytochemistry, the relative coupling rates with naphthols were tested as well as the solubility of the resulting azo dyes. Three of the diazotates were reacted with tissue proteins in aldehyde-fixed material, and the density increases thus produced were compared in the electron microscope with those produced by staining similarly fixed material with the phthalocyanin dye, Alcian Blue. Finally, one of the diazotates was used as a capture reagent for the demonstration of the sites of acid phosphatase activity with the electron microscope.

Desiccation causes the proliferation of multicellular hairs, but not mucilage papillae, in Cryptothallus mirabilis (Hepatophyta): a correlated light and electron microscope study

1990 ◽  
Vol 68 (3) ◽  
pp. 697-706 ◽  
Author(s):  
Jeffrey G. Duckett ◽  
Karen S. Renzaglia ◽  
Keith Pell
Keyword(s):  
Endoplasmic Reticulum ◽  
Electron Microscope ◽  
Acid Phosphatase ◽  
Endophytic Fungus ◽  
Electron Microscope Study ◽  
Secretory Phase ◽  
Golgi Bodies ◽  
Dense Cytoplasm ◽  
Thin Walled ◽  
Multicellular Hair

When Cryptothallus dries out over periods of 4–20 days, the dorsal surfaces of the thalli become covered with multicellular hairs. The distribution of mucilage papillae and the endophytic fungus are not affected by desiccation. The hairs are thin walled and highly vacuolated whereas the mucilage papillae, like their secretory counterparts in Marchantia and mosses, are thick walled with dense cytoplasm containing stacks of endoplasmic reticulum and numerous Golgi bodies. Cytochemistry shows that the secretion is rich in carbohydrates and is derived from Golgi vesicles. After an active secretory phase, senescence of the mucilage papillae is associated with acid phosphatase activity. Key words: Aneura, Cryptothallus, desiccation, liverwort, mucilage papilla, multicellular hair, ultrastructure.

scholarly journals A NEW CRYSTAL-CONTAINING CELL IN HUMAN ADRENAL CORTEX

1972 ◽  
Vol 55 (1) ◽  
pp. 126-133 ◽  
Author(s):  
Maria C. Magalhães
Keyword(s):  
Electron Microscope ◽  
Acid Phosphatase ◽  
Adrenal Cortex ◽  
Human Subjects ◽  
Microscope Examination ◽  
Adrenal Androgen ◽  
Spindle Shaped Cell ◽  
Phosphatase Activities ◽  
Shaped Cell ◽  
Testicular Interstitial Cells

Electron microscope examination of the adrenal cortex from three male human subjects revealed a special type of cell occurring in periendothelial spaces, in all adrenal cortex zones. It is a clear, spindle-shaped cell the principal cytoplasmic features of which are crystalline inclusions with a structure similar to that of the Reinke crystals of human testicular interstitial cells and an abundance of microfilaments. Enzymatic digestions with pronase, pepsin, and ribonuclease were performed, and no digestion of the crystals was obtained. The crystals had no peroxidase or acid phosphatase activities. This cell appears to be exclusive to human males and it may be related to adrenal androgen secretion.

The host-parasite interface of Cyathocotyle bushiensis Khan, 1962 (Trematoda: Strigeoidea)

Parasitology ◽  
1968 ◽  
Vol 58 (2) ◽  
pp. 371-375 ◽  
Author(s):  
David A. Erasmus
Keyword(s):  
Electron Microscope ◽  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Plasma Membranes ◽  
Science Research ◽  
Strong Acid ◽  
Gland Cells ◽  
Adhesive Organ ◽  
Cyathocotyle Bushiensis

A combination of histochemical and electron microscope techniques have demonstrated, in Cyathocotyle bushiensis, alkaline phosphatase activity in the matrix of the tegument, in the distal and basal plasma membranes of the tegument, in the wall of the ducts extending from the adhesive organ gland cells and in the wall of the adhesive organ microvilli. Acid phosphatase activity was much stronger and was present in the tegument matrix and in the granular component of the secretion from the adhesive organ gland cells. Strong acid phosphatase activity was also present in the cisternae of the endoplasmic reticulum of the adhesive organ gland cells.I am greatly indebted to Professor Brough for the excellent facilities available within this department. I also wish to thank Professor J. Sinclair (Department of Mining) for electron microscope facilities extended to me in the early stages of this investigation, and to Mr W. Henderson, Mr T. Davies and Miss M. Williams for their invaluable assistance. The purchase of the Huxley ultramicrotome, coating unit and an AEI EM 6 electron microscope was made possible by a grant from the Science Research Council.

Sign in / Sign up

Export Citation Format

Share Document