Detection of Structure Characteristics and Its Discontinuity Based Field Programmable Gate Array Processor in Cancer Cell by Wavelet Transform

Digital clinical histopathology is one of the crucial techniques for precise cancer cell diagnosing in modern medicine. The Synovial Sarcoma (SS) cancer cell patterns seem to be a spindle shaped cell (SSC) structure and it is very difficult to identify the exact oval shaped cell structure through pathologist’s eye perception. Meanwhile, there is necessitating for monitoring and securing the successful and effective image data processing in the the huge network data which is also a complex one. A field programmable Gate Array (FPGA) was regarded as a necessary one for this. In this work, based on FPGA a Cancer Cell classification is made for the regulation and execution. Hence, mathematically the SSC regularity structures and its discontinuities are measured by the holder exponent (HE) function. In this research work, HE values have been determined by Wavelet Transform Modulus Maxima (WTMM) and Wavelet Leader (WL) methods with basis function of Haar wavelet based on FPGA Processor. The quantitative parameters such as Mean of Asymptotic Discontinuity (MAD), Mean of Removable Discontinuity (MRD) and Number of Discontinuity Points (NDPs) have been considered to determine the prediction of discontinuity detection between WTMM and WL methods. With the help of receiver operating characteristics (ROC) curve, the significant difference of discontinuity detection performance between both the methods has been analyzed. From the experimental results, it is clear that the WL method is more practically feasible and it gives satisfactory performance, in terms of sensitivity and specificity percentage values, which are 80.56% and 59.46%, respectively in the blue color components of the SNR 20 dB noise image.

Vascular lung triculture organoid via soluble extracellular matrix suspension

Scaffold-free tissue engineering is desired in creating consistently sized and shaped cell aggregates but has been limited to spheroid-like structure and function, thus restricting its use in accurate disease modeling. Here, we show formation of a viable lung organoid from epithelial, endothelial, and fibroblast stable cell lines in suspension culture supplemented with soluble concentrations of extracellular matrix proteins (ECM). We demonstrate the importance of soluble ECM in organotypic patterning with the emergence of air space-like gas exchange units, formation of branching, perfusable vasculature, and increased 3D growth. Our results show a dependent relationship between enhanced fibronectin fibril assembly and the incorporation of ECM in the organoid. Endothelial branching was found to depend on both soluble ECM and fibroblast. We successfully applied this technology in modeling lung fibrosis via bleomycin induction and test a potential antifibrotic drug in vitro while maintaining fundamental cell-cell interactions in lung tissue. Our human fluorescent lung organoid (hFLO) model accurately represents features of pulmonary fibrosis which were ameliorated by fasudil treatment. We demonstrate a 3D culture method with potential of creating organoids from mature cells, thus opening avenues for disease modeling and regenerative medicine, enhancing understanding of lung cell biology in health and lung disease.

A Cell’s Viscoelasticity Measurement Method Based on the Spheroidization Process of Non-Spherical Shaped Cell

The mechanical properties of biological cells, especially the elastic modulus and viscosity of cells, have been identified to reflect cell viability and cell states. The existing measuring techniques need additional equipment or operation condition. This paper presents a cell’s viscoelasticity measurement method based on the spheroidization process of non-spherical shaped cell. The viscoelasticity of porcine fetal fibroblast was measured. Firstly, we introduced the process of recording the spheroidization process of porcine fetal fibroblast. Secondly, we built the viscoelastic model for simulating a cell’s spheroidization process. Then, we simulated the spheroidization process of porcine fetal fibroblast and got the simulated spheroidization process. By identifying the parameters in the viscoelastic model, we got the elasticity (500 Pa) and viscosity (10 Pa·s) of porcine fetal fibroblast. The results showed that the magnitude of the elasticity and viscosity were in agreement with those measured by traditional method. To verify the accuracy of the proposed method, we imitated the spheroidization process with silicone oil, a kind of viscous and uniform liquid with determined viscosity. We did the silicone oil’s spheroidization experiment and simulated this process. The simulation results also fitted the experimental results well.

Establishment of Three-Dimensional Bioprinted Bladder Cancer-on-a-Chip with a Microfluidic System Using Bacillus Calmette–Guérin

Immunotherapy of bladder cancer is known to have favorable effects, although it is difficult to determine which patients will show a good response because of the different tumor microenvironments (TME). Here, we developed a bladder cancer-on-a-chip (BCOC) to mimic the TME using three-dimensional (3D) bioprinting and microfluidic technology. We fabricated a T24 and a 5637-cell line-based BCOC that also incorporated MRC-5, HUVEC, and THP-1 cells. We evaluated the effects of TME and assessed the immunologic reactions in response to different concentrations of Bacillus Calmette–Guérin (BCG) via live/dead assay and THP-1 monocytic migration, and concentrations of growth factors and cytokines. The results show that cell viability was maintained at 15% filling density in circle-shaped cell constructs at 20 μL/min microfluidic flow rate. A 3D co-culture increased the proliferation of BCOCs. We found that the appropriate time to evaluate the viability of BCOC, concentration of cytokines, and migration of monocytes was 6 h, 24 h, and three days after BGC treatment. Lastly, the immunotherapeutic effects of BCOC increased according to BCG dosage. To predict effects of immunotherapeutic agent in bladder cancer, we constructed a 3D bioprinted BCOC model. The BCOC was validated with BCG, which has been proven to be effective in the immunotherapy of bladder cancer.

Simulation of Shear-Thickening Liquid Transfer between U-Shaped Cell and Flat Plate

Based on the actual measurement of the shear-thickening properties of water-based inks, in order to improve the ink transfer rate, the PLIC (Piecewise Linear Interface Construction) interface tracking method and the VOF (Volume of Fluid) method are used to simulate the transfer process of the shear-thickening liquid between the U-shaped cell and the upwardly moving plate. The effects of substrate surface wettability, cell contact angle, and cell depth on liquid transfer were studied. The results showed that all can increase the liquid transfer rate, and the change of the cell contact angle also led to the difference in the breaking time of the liquid filament. In addition, the shallow plate effect was discovered in the study of cell depth. The shallow plate effect is a phenomenon by which the amount of liquid transferred and the liquid transfer rate are greatly improved when the depth of the cell decreases to a certain limit value. In addition, for the U-shaped cell, the optimization method combining the shallow printing plate effect and fillet can greatly improve the liquid transfer rate and solve the undesirable problems such as plate blocking. After optimization, a liquid transfer rate of about 85% can be achieved.

An update on the rod microglia variant in experimental and clinical brain injury and disease

Contemporary microglia morphologies include ramified, activated, and amoeboid, with the morphology of microglia considered highly coupled to the cellular function. Rod microglia are an additional activated microglia variant observed in the aging, injured, and diseased brain. Rod microglia were reported frequently in the early 1900’s by neuropathologists in post-mortem cases of general paresis, Alzheimer’s disease, and encephalitis, and then remained largely ignored for almost 100 years. Recent reports have renewed interest in rod microglia, most notably after experimental traumatic brain injury. Rod microglia are formed by the narrowing of the soma and retraction of planar processes, which results in the appearance of an elongated, rod-shaped cell. Rod microglia are most commonly observed in the cortex, aligned perpendicular to the dural surface adjacent to neuronal processes, and the hippocampus, aligned perpendicular to hippocampal layers. Further, rod microglia form trains with one another, apical end to basal end. By replicating the process of sketching microscopic observation, rod microglia are redefined by circumnutation around the long axis. In this update, we summarize the rod microglia variant in clinical and experimental literature and advocate for investigation into mechanisms of rod microglia origin and function.