Sesquiterpene Synthase-Catalyzed Conversion of a Farnesyl Diphosphate Analogue to a Nonnatural Terpenoid Ether

2018 ◽  
pp. 83-95 ◽  
Author(s):  
Florence Huynh ◽  
David J. Miller ◽  
Rudolf K. Allemann
Keyword(s):  
Farnesyl Diphosphate ◽  
Sesquiterpene Synthase

scholarly journals Genome mining in Trichoderma viride J1-030: discovery and identification of novel sesquiterpene synthase and its products

2019 ◽  
Vol 15 ◽  
pp. 2052-2058 ◽  
Author(s):  
Xiang Sun ◽  
You-Sheng Cai ◽  
Yujie Yuan ◽  
Guangkai Bian ◽  
Ziling Ye ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Nmr Spectroscopy ◽  
Filamentous Fungi ◽  
Trichoderma Viride ◽  
Genome Mining ◽  
2D Nmr ◽  
Farnesyl Diphosphate ◽  
2D Nmr Spectroscopy ◽  
Sesquiterpene Synthase

Sesquiterpene synthases in Trichoderma viride have been seldom studied, despite the efficiency of filamentous fungi for terpenoid production. Using the farnesyl diphosphate-overexpressing Saccharomyces cerevisiae platform to produce diverse terpenoids, we herein identified an unknown sesquiterpene synthase from T. viride by genome mining and determined the structure of its corresponding products. One new 5/6 bicyclic sesquiterpene and its esterified derivative were characterised by GC–MS and 1D and 2D NMR spectroscopy. To the best of our knowledge, this is the first well-identified sesquiterpene synthase from T. viride to date.

scholarly journals Sesquiterpene Synthase‐Catalysed Formation of a New Medium‐Sized Cyclic Terpenoid Ether from Farnesyl Diphosphate Analogues

ChemBioChem ◽  
2018 ◽  
Vol 19 (17) ◽  
pp. 1834-1838 ◽  
Author(s):  
Florence Huynh ◽  
Daniel J. Grundy ◽  
Robert L. Jenkins ◽  
David J. Miller ◽  
Rudolf K. Allemann
Keyword(s):  
Farnesyl Diphosphate ◽  
Sesquiterpene Synthase

scholarly journals Improved selectivity of an engineered multi-product terpene synthase

2014 ◽  
Vol 12 (23) ◽  
pp. 4013-4020 ◽  
Author(s):  
Ryan Lauchli ◽  
Julia Pitzer ◽  
Rebekah Z. Kitto ◽  
Karolina Z. Kalbarczyk ◽  
Kersten S. Rabe
Keyword(s):  
Farnesyl Diphosphate ◽  
Terpene Synthase ◽  
Kinetic Properties ◽  
Natural Substrate ◽  
Wild Type ◽  
Random Mutation ◽  
Sesquiterpene Synthase ◽  
Germacrene D

Random mutation of the multi-product sesquiterpene synthase Cop2 generated a mutant that converted the natural substrate farnesyl diphosphate (FPP) into germacrene D-4-ol with 77% selectivity without detrimental effects on wild-type kinetic properties.

scholarly journals The santalene synthase from Cinnamomum camphora: Reconstruction of a sesquiterpene synthase from a monoterpene synthase

2020 ◽  
Vol 695 ◽  
pp. 108647
Author(s):  
Alice Di Girolamo ◽  
Janani Durairaj ◽  
Adèle van Houwelingen ◽  
Francel Verstappen ◽  
Dirk Bosch ◽  
...  
Keyword(s):  
Cinnamomum Camphora ◽  
Sesquiterpene Synthase ◽  
Monoterpene Synthase
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