A solid-phase immunoassay of protease-resistant prion protein with filtration blotting involving sodium dodecyl sulfate

2006 ◽  
Vol 349 (2) ◽  
pp. 218-228 ◽  
Author(s):  
Yoshiteru Kobayashi ◽  
Naoyuki Kohno ◽  
Shoko Wanibe ◽  
Kazunari Hirayasu ◽  
Hitoshi Uemori ◽  
...  
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Prion Protein ◽  
Solid Phase ◽  
Sodium Dodecyl ◽  
Solid Phase Immunoassay ◽  
Dodecyl Sulfate

scholarly journals Solid phase extraction of trace amounts of cadmium(II) ions in water and food samples using iron magnetite nanoparticles modified by sodium dodecyl sulfate and 2-mercaptobenzothiazole

2017 ◽  
Vol 75 (6) ◽  
pp. 1440-1446
Author(s):  
Shahriar Abbasi ◽  
Mehdi ShanbehDehbalai ◽  
Hossein Khani
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Solid Phase Extraction ◽  
Magnetite Nanoparticles ◽  
Solid Phase ◽  
Sodium Dodecyl ◽  
Food Samples ◽  
Phase Extraction ◽  
Cadmium Ions ◽  
Relative Standard ◽  
Dodecyl Sulfate

A new, simple and rapid method for solid phase extraction and preconcentration of trace amounts of cadmium ions using 2-mercaptobenzothiazole/sodium dodecyl sulfate immobilized on magnetite nanoparticles (MBT-SDS-MNPs) was proposed. The method is based on the extraction of cadmium ions via complexation with MBT immobilized on SDS-coated MNPs and their determination by flame atomic absorption spectrometry. The effects of different parameters – pH; eluent type, concentration and volume; amounts of salt and adsorbent; contact time and interfering ions – on the adsorption of cadmium ions were studied. Under optimized conditions, the calibration curve was linear in the range of 10–5,000 μg L−1. Detection limit and relative standard deviation of the proposed method were 0.009 μg L−1 and 2.2%, respectively. The adsorption data were analyzed by Langmuir and Freundlich isotherm models and a maximum adsorption amount of 24.80 mg g−1, a Langmuir adsorption equilibrium constant (b) of 4.62 and Freundlich constants Kf and n of 6.075 mg1–1/n L1/n g−1 and 2.391, respectively, were obtained. Finally, this adsorbent was successfully used for extraction of cadmium from water and food samples.

Impact of SDS surfactant on the interactions of Cu2+ ions with the amyloidogenic region of human prion protein

2015 ◽  
Vol 44 (29) ◽  
pp. 13125-13132 ◽  
Author(s):  
Aleksandra Hecel ◽  
Caterina Migliorini ◽  
Daniela Valensin ◽  
Marek Luczkowski ◽  
Henryk Kozlowski
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Prion Protein ◽  
Anionic Surfactant ◽  
Sodium Dodecyl ◽  
Dodecyl Sulfate ◽  
Amyloidogenic Region ◽  
Human Prion Protein ◽  
Sds Surfactant

In this work, we focused on Cu2+ interaction with the hPrP fragment spanning residues 91–127 in the presence of micelles formed by the anionic surfactant sodium dodecyl sulfate.

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