A fluorescence polarization assay to quantify biotin and biotin-binding proteins in whole plant extracts using Alexa-Fluor 594 biocytin

2008 ◽  
Vol 381 (1) ◽  
pp. 107-112 ◽  
Author(s):  
Harry Martin ◽  
Colleen Murray ◽  
John Christeller ◽  
Tony McGhie
Keyword(s):  
Plant Extracts ◽  
Fluorescence Polarization ◽  
Binding Proteins ◽  
Alexa Fluor ◽  
Whole Plant ◽  
Biotin Binding ◽  
Fluorescence Polarization Assay

scholarly journals A 1536-Well Fluorescence Polarization Assay to Screen for Modulators of the MUSASHI Family of RNA-Binding Proteins

2014 ◽  
Vol 17 (7) ◽  
pp. 596-609 ◽  
Author(s):  
Gerard Minuesa ◽  
Christophe Antczak ◽  
David Shum ◽  
Constantin Radu ◽  
Bhavneet Bhinder ◽  
...  
Keyword(s):  
Fluorescence Polarization ◽  
Binding Proteins ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Fluorescence Polarization Assay

Development of a fluorescence polarization assay to screen for inhibitors of the FtsZ/ZipA interaction

2003 ◽  
Vol 323 (2) ◽  
pp. 224-233 ◽  
Author(s):  
Cynthia Hess Kenny ◽  
Weidong Ding ◽  
Kerry Kelleher ◽  
Susan Benard ◽  
Elizabeth Glasfeld Dushin ◽  
...  
Keyword(s):  
Fluorescence Polarization ◽  
Fluorescence Polarization Assay

Expression of various biotin-binding proteins in transgenic tobacco confers resistance to potato tuber moth, Phthorimaea operculella (Zeller) (fam. Gelechiidae)

2010 ◽  
Vol 19 (6) ◽  
pp. 1041-1051 ◽  
Author(s):  
Colleen Murray ◽  
Ngaire P. Markwick ◽  
Ryohei Kaji ◽  
Joanne Poulton ◽  
Harry Martin ◽  
...  
Keyword(s):  
Potato Tuber ◽  
Transgenic Tobacco ◽  
Binding Proteins ◽  
Phthorimaea Operculella ◽  
Potato Tuber Moth ◽  
Biotin Binding

A Fluorescence Polarization Assay for Cyclic Nucleotide Phosphodiesterases

2002 ◽  
Vol 7 (3) ◽  
pp. 215-222 ◽  
Author(s):  
Wei Huang ◽  
Yan Zhang ◽  
J. Richard Sportsman
Keyword(s):  
Cyclic Nucleotide ◽  
Fluorescence Polarization ◽  
Drug Targets ◽  
Second Messengers ◽  
Reaction Products ◽  
Cyclic Nucleotide Phosphodiesterases ◽  
Extracellular Signals ◽  
Fluorescence Polarization Assay ◽  
Hydrolysis Of ◽  
Nucleotide Phosphodiesterases

Cyclic nucleotide phosphodiesterases (PDEs) catalyze the hydrolysis of the 3′-ester bond of cyclic AMP (cAMP) and cyclic GMP (cGMP), important second messengers in the transduction of a variety of extracellular signals. There is growing interest in the study of PDEs as drug targets for novel therapeutics. We describe the development of a homogeneous fluorescence polarization assay for PDEs based on the strong binding of PDE reaction products (i.e., AMP or GMP) onto modified nanoparticles through interactions with immobilized trivalent metal cations. This assay technology (IMAP) is applicable to both cAMP- and cGMP-specific PDEs. Results of the assay in 384- and 1536-well microplates are presented.

Assessment of fluorescence polarization assay: a candid diagnostic tool inBrucella abortusstrain 19 vaccinated areas

2018 ◽  
Vol 62 (11) ◽  
pp. 694-701 ◽  
Author(s):  
Triveni Kalleshamurthy ◽  
Ranjitha Shekar ◽  
Harish Heballi Niranjanamurthy ◽  
Krithiga Natesan ◽  
Bibek Ranjan Shome ◽  
...  
Keyword(s):  
Diagnostic Tool ◽  
Fluorescence Polarization ◽  
Fluorescence Polarization Assay

A Fluorescence Polarization Assay To Detect Steroid Hormone Traces in Milk

2015 ◽  
Vol 63 (41) ◽  
pp. 9159-9164 ◽  
Author(s):  
Antonio Varriale ◽  
Anna Pennacchio ◽  
Gabriella Pinto ◽  
Giorgia Oliviero ◽  
Stefano D’Errico ◽  
...  
Keyword(s):  
Fluorescence Polarization ◽  
Steroid Hormone ◽  
Fluorescence Polarization Assay
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