Structural characterization of CYP165D3, a cytochrome P450 involved in phenolic coupling in teicoplanin biosynthesis

2011 ◽  
Vol 507 (1) ◽  
pp. 163-173 ◽  
Author(s):  
Max J. Cryle ◽  
Jessica Staaden ◽  
Ilme Schlichting
2021 ◽  
Author(s):  
Tobias A. M. Gulder ◽  
Hülya Aldemir ◽  
Shuangjie Shu ◽  
Francoise Schaefers ◽  
Hanna Hong ◽  
...  

2012 ◽  
Vol 287 (53) ◽  
pp. 44581-44591 ◽  
Author(s):  
R. Leila Reynald ◽  
Stefaan Sansen ◽  
C. David Stout ◽  
Eric F. Johnson

2010 ◽  
Vol 286 (7) ◽  
pp. 5736-5743 ◽  
Author(s):  
An Wang ◽  
Uzen Savas ◽  
C. David Stout ◽  
Eric F. Johnson

Author(s):  
S. F. Hayes ◽  
M. D. Corwin ◽  
T. G. Schwan ◽  
D. W. Dorward ◽  
W. Burgdorfer

Characterization of Borrelia burgdorferi strains by means of negative staining EM has become an integral part of many studies related to the biology of the Lyme disease organism. However, relying solely upon negative staining to compare new isolates with prototype B31 or other borreliae is often unsatisfactory. To obtain more satisfactory results, we have relied upon a correlative approach encompassing a variety EM techniques, i.e., scanning for topographical features and cryotomy, negative staining and thin sectioning to provide a more complete structural characterization of B. burgdorferi.For characterization, isolates of B. burgdorferi were cultured in BSK II media from which they were removed by low speed centrifugation. The sedimented borrelia were carefully resuspended in stabilizing buffer so as to preserve their features for scanning and negative staining. Alternatively, others were prepared for conventional thin sectioning and for cryotomy using modified procedures. For thin sectioning, the fixative described by Ito, et al.


2011 ◽  
Vol 44 (06) ◽  
Author(s):  
A Bracher ◽  
C Kozany ◽  
AK Thost ◽  
F Hausch

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