Characterisation of the substituent distribution in starch and cellulose derivatives

The complexity of the substituent distribution in polysaccharide derivatives is discussed and defined. The challenges regarding analytical characterization that results from various interrelated categories of distributions, including molecular weight, chemical composition, and microstructure, are outlined. Due to these convoluted levels of complexity, results should always be interpreted with carefulness. Various analytical approaches which have been applied to starch and cellulose derivatives are recapped, including enzymatic, mass spectrometric, and chromatographic methods. The relation of heterogeneities of first and second order among and along the polysaccharide chains is addressed. Finally, examples of own analytical work on cellulose ethers are presented, including the MS analysis of methyl cellulose (MC) blends and fractionation studies of fully esterified MC, especially its 4-methoxybenzoates by gradient HPLC on normal phase. Preparative fractionation according to the degree of substitution (DS) allows follow-up analysis in order to get more detailed information on the substituent distribution in such sub-fractions.

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Ultra-thin layers of phosphorylated cellulose derivatives on metal surfaces

Macromolecular Symposia ◽

10.1002/1521-3900(200201)177:1<97::aid-masy97>3.0.co;2-# ◽

2002 ◽

Vol 177 (1) ◽

pp. 97-110 ◽

Cited By ~ 5

Author(s):

Evelin Jaehne ◽

Thomas Kowalik ◽

Hans-Juergen P. Adler ◽

Andreas Plagge ◽

Martin Stratmann

Keyword(s):

Metal Surfaces ◽

Thin Layers ◽

Cellulose Derivatives

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Improved Release of Celecoxib from High Drug Loading Amorphous Solid Dispersions Formulated with Polyacrylic Acid and Cellulose Derivatives

10.26226/morressier.57d6b2bbd462b8028d88d1d4 ◽

2016 ◽

Author(s):

Tian Xie

Keyword(s):

Polyacrylic Acid ◽

Drug Loading ◽

Solid Dispersions ◽

Amorphous Solid ◽

Cellulose Derivatives ◽

Amorphous Solid Dispersions ◽

High Drug ◽

High Drug Loading

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Analytical Study of Irradiated Cellulose Derivatives

Current Pharmaceutical Analysis ◽

10.2174/1573412911309030004 ◽

2013 ◽

Vol 9 (3) ◽

pp. 252-260

Author(s):

M. Stawny ◽

K. Dettlaff ◽

B. Marciniec ◽

E. Jaroszkiewicz ◽

B. Czajka ◽

...

Keyword(s):

Analytical Study ◽

Cellulose Derivatives

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Purification of Glycerol Kinase by "Dye-Ligand" Chromatography and Hydrophobic Interaction Chromatography on Bead-Cellulose Derivatives

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19930445 ◽

1993 ◽

Vol 58 (2) ◽

pp. 445-451 ◽

Cited By ~ 7

Author(s):

Vladimír Žúbor ◽

Albert Breier ◽

Marta Horváthová ◽

Dagmar Hagarová ◽

Peter Gemeiner ◽

...

Keyword(s):

Hydrophobic Interaction ◽

Specific Activity ◽

Hydrophobic Interaction Chromatography ◽

Glycerol Kinase ◽

Enzymic Activity ◽

Cellulose Derivatives ◽

Long Term Storage ◽

Bead Cellulose ◽

Term Storage

The crude extract of cytosole enzymes was obtained from homogenized cells of Saccharomyces cerevisiae by partition. The enzyme was then isolated from the lower aqueous phase displaying higher glycerol kinase activity by dye-ligand chromatography on Cibacron Blue (CB) or Remazol Brilliant Blue R (RB)-derivatized bead-cellulose, ATP being the eluent. The specific activity of glycerol kinase rised more than 10 and 7-times after affinity dye-ligand chromatography and hydrophobic interaction chromatography, respectively. Glycerol kinase obtained by the latter method was purified by CB-bead cellulose. The final preparation maintained its enzymic activity without noticeable losses during a long-term storage at 4 °C in dark.

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