Analysis of protein-based binding media found in paintings using laser induced fluorescence spectroscopy

2006 ◽  
Vol 573-574 ◽  
pp. 341-346 ◽  
Author(s):  
Austin Nevin ◽  
Sharon Cather ◽  
Demetrios Anglos ◽  
Costas Fotakis
2006 ◽  
Vol 2006 ◽  
pp. 1-5 ◽  
Author(s):  
Austin Nevin ◽  
Demetrios Anglos

Laser-induced fluorescence (LIF) spectroscopy can provide nondestructive, qualitative analysis of protein-based binding media found in artworks. Fluorescence emissions from proteins in egg yolk and egg white are due to autofluorescent aromatic amino acids as well as other native and age-related fluorophores, but the potential of fluorescence spectroscopy for the differentiation between binding media is dependent on the choice of a suitable excitation wavelength and limited by problems in interpretation. However, a better understanding of emission spectra associated with LIF can be achieved following comparisons with total emission fluorescence spectra where a series of consecutive emission spectra are recorded over a specific range. Results using nanosecond UV laser sources for LIF of egg-based binding media are presented which are rationalised following comparisons with total emission spectra. Specifically, fluorescence is assigned to tryptophan and oxidation products of amino acids; in the case of egg yolk, fatty-acid polymerisation and age-related degradation products account for the formation of fluorophores.


2021 ◽  
Vol 211 ◽  
pp. 105001
Author(s):  
Amanda M. Tadini ◽  
Alfredo A.P. Xavier ◽  
Débora M.B.P. Milori ◽  
Patrícia P.A. Oliveira ◽  
José R. Pezzopane ◽  
...  

2004 ◽  
Vol 120 (6) ◽  
pp. 2662-2666 ◽  
Author(s):  
Masakazu Nakajima ◽  
Yu Yoneda ◽  
Yoshihiro Sumiyoshi ◽  
Yasuki Endo

1986 ◽  
Vol 39 (2) ◽  
pp. 91-95 ◽  
Author(s):  
H. F. D�bele ◽  
M. H�rl ◽  
M. R�wekamp ◽  
B. Reimann

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