scholarly journals A multiplex PCR protocol for rapid differential identification of four families of trematodes with medical and veterinary importance transmitted by Biomphalaria Preston, 1910 snails

Acta Tropica ◽  
2020 ◽  
Vol 211 ◽  
pp. 105655
Author(s):  
Silvia Gonçalves Mesquita ◽  
Gabriela Flávia Rodrigues-Luiz ◽  
João Luís Reis-Cunha ◽  
Mariana Santos Cardoso ◽  
Cristiane Lafetá Furtado De Mendonça ◽  
...  
2010 ◽  
Vol 48 (9) ◽  
pp. 3111-3116 ◽  
Author(s):  
B. Wicht ◽  
T. Yanagida ◽  
T. Scholz ◽  
A. Ito ◽  
J. A. Jimenez ◽  
...  

2017 ◽  
Vol 61 (6) ◽  
Author(s):  
Rathina Kumar Shanmugakani ◽  
Yukihiro Akeda ◽  
Norihisa Yamamoto ◽  
Noriko Sakamoto ◽  
Hideharu Hagiya ◽  
...  

ABSTRACT A PCR-dipstick chromatography technique was designed and evaluated for differential identification of bla NDM, bla KPC, bla IMP, and bla OXA-48 carbapenemase genes directly in stool specimens within 2 h. It is a DNA-DNA hybridization-based detection system where PCR products can be easily interpreted by visual observation without electrophoresis. The PCR-dipstick showed high sensitivity (93.3%) and specificity (99.1%) in directly detecting carbapenemase genes in stool specimens compared with multiplex PCR for genomic DNA of the isolates from those stool specimens.


2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Soumya Paul ◽  
Bhavani Venkataswamachari Peddayelachagiri ◽  
Madhurjya Gogoi ◽  
Sowmya Nagaraj ◽  
Shylaja Ramlal ◽  
...  

2009 ◽  
Vol 138 (4) ◽  
pp. 525-533 ◽  
Author(s):  
M. J. FARFÁN ◽  
T. A. GARAY ◽  
C. A. PRADO ◽  
I. FILLIOL ◽  
M. T. ULLOA ◽  
...  

SUMMARYMost of the multiplex PCR (mPCR) used to identifyShigellado not discriminate betweenShigellaspecies or serotypes. We designed a mPCR to differentiate betweenS. flexneriandS. sonneistrains based on the detection of markers associated with theshepathogenicity island described inShigella. In addition, specific primers were included to detect theShigellavirulence determinants ShET-1 and ShET-2 enterotoxin genes. The analysis of 304Shigellastrains from Chile and 79Shigellastrains from other geographic locations indicated that the mPCR described here detected allShigellaspecies and specifically differentiatedS. flexneriandS. sonnei. The technique was sensitive, reproducible, specific and simple to perform, providing a new tool with the potential to be employed for epidemiological and diagnostic purposes.


2007 ◽  
Vol 73 (10) ◽  
pp. 3380-3390 ◽  
Author(s):  
Daniel Müller ◽  
Lilo Greune ◽  
Gerhard Heusipp ◽  
Helge Karch ◽  
Angelika Fruth ◽  
...  

ABSTRACT Intestinal pathogenic Escherichia coli represents a global health problem for mammals, including humans. At present, diarrheagenic E. coli bacteria are grouped into seven major pathotypes that differ in their virulence factor profiles, severity of clinical manifestations, and prognosis. In this study, we developed and evaluated a one-step multiplex PCR (MPCR) for the straightforward differential identification of intestinal pathotypes of E. coli. The specificity of this novel MPCR was validated by using a subset of reference strains and further confirmed by PCR-independent pheno- and genotypic characterization. Moreover, we tested 246 clinical E. coli isolates derived from diarrhea patients from several distinct geographic regions. Interestingly, besides strains belonging to the defined and well-described pathotypes, we identified five unconventional strains expressing intermediate virulence factor profiles. These strains have been further characterized and appear to represent intermediate strains carrying genes and expressing factors associated with enteropathogenic E. coli, Shiga toxin-producing E. coli, enterotoxigenic E. coli, and enteroaggregative E. coli alike. These strains represent further examples of the extraordinary plasticity of the E. coli genome. Moreover, this implies that the important identification of specific pathotypes has to be based on a broad matrix of indicator genes. In addition, the presence of intermediate strains needs to be accounted for.


1999 ◽  
Vol 30 (4) ◽  
pp. 322-323 ◽  
Author(s):  
A. Barroso ◽  
S. Dunner ◽  
J. Ca·ón
Keyword(s):  

2005 ◽  
Vol 127 (03) ◽  
Author(s):  
J Rom ◽  
A Schneeeweiss ◽  
V Zieglschmid ◽  
C Hollmann ◽  
O Böcher ◽  
...  
Keyword(s):  

2016 ◽  
Vol 1 (2) ◽  
pp. 38-42 ◽  
Author(s):  
Khairun Nessa ◽  
Dilruba Ahmed ◽  
Johirul Islam ◽  
FM Lutful Kabir ◽  
M Anowar Hossain

A multiplex PCR assay was evaluated for diagnosis of diarrheagenic Escherichia coli in stool samples of patients with diarrhoea submitted to a diagnostic microbiology laboratory. Two procedures of DNA template preparationproteinase K buffer method and the boiling method were evaluated to examine isolates of E. coli from 150 selected diarrhoeal cases. By proteinase K buffer method, 119 strains (79.3%) of E. coli were characterized to various categories by their genes that included 55.5% enteroaggregative E. coli (EAEC), 18.5% enterotoxigenic E. coli (ETEC), 1.7% enteropathogenic E. coli (EPEC), and 0.8% Shiga toxin-producing E. coli (STEC). Although boiling method was less time consuming (<24 hrs) and less costly (<8.0 US $/ per test) but was less efficient in typing E. coli compared to proteinase K method (41.3% vs. 79.3% ; p<0.001). The sensitivity and specificity of boiling method compared to proteinase K method was 48.7% and 87.1% while the positive and negative predictive value was 93.5% and 30.7%, respectively. The majority of pathogenic E. coli were detected in children (78.0%) under five years age with 53.3% under one year, and 68.7% of the children were male. Children under 5 years age were frequently infected with EAEC (71.6%) compared to ETEC (24.3%), EPEC (2.7%) and STEC (1.4%). The multiplex PCR assay could be effectively used as a rapid diagnostic tool for characterization of diarrheagenic E. coli using a single reaction tube in the clinical laboratory setting.Bangladesh J Med Microbiol 2007; 01 (02): 38-42


2015 ◽  
Vol 14 (1) ◽  
pp. 282-288
Author(s):  
Israa Adnan Ibraheam Al-Baghdady ◽  
Ashwak Bassim Jassim ◽  
Zainab Khudher Ahmed

1968 ◽  
Vol 15 (3) ◽  
pp. 376-383 ◽  
Author(s):  
Victor M. Matthews

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