Molecular detection and genotype diversity of hemoplasmas in non-hematophagous bats and associated ectoparasites sampled in peri-urban areas from Brazil

From 2016 to 2018, ticks were collected from 272 dogs admitted to veterinary clinics in the city of Olsztyn (north-eastern Poland). Among 522 collected ticks, 423 were identified as Ixodes ricinus (413 females and 10 males) and 99 as Dermacentor reticulatus (62 females and 37 males). Non-engorged (86 individuals) and engorged (436 individuals) ticks were screened for the presence of Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum DNA. Borrelia and A. phagocytophilum species detection was determined based on the sequence of the fla B and 16S RNA genes, respectively. DNA of B. burgdorferi s.l. was identified in 31.6% (165/522, 95% CI: 27.6–35.8%) of ticks (I. ricinus 151/423, 35.7%, 95% CI: 31.1–40.4%; D. reticulates 14/99, 14.1%, 95% CI: 7.9–22.6%). A. phagocytophilum was identified in 0.96% (5/522, 95% CI: 0.3–2.2%) of specimens. All positive samples were engorged I. ricinus females (5/402, 1.2%, 95% CI: 0.4–2.9%). In 85.4% (141/165, 95% CI: 79.1–90.4%) of Borrelia infected ticks, the DNA of one genospecies was revealed. The DNA of at least two different genospecies was detected in 14.5% of specimens (24/165, 95% CI: 9.5–20.8). The coexistence of B. burgdorferii s.l. and A. phagocytophilum was not detected.

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Phlebotominae Fauna (Diptera: Psychodidae) and Molecular Detection of Leishmania (Kinetoplastida: Trypanosomatidae) in Urban Caves of Belo Horizonte, Minas Gerais, Brazil

Journal of Medical Entomology ◽

10.1093/jme/tjab156 ◽

2021 ◽

Author(s):

Paula Cavalcante Lamy Serra e Meira ◽

Bruna Lacerda Abreu ◽

Ana Paula Lusardo de Almeida Zenóbio ◽

Cristiani de Castilho Sanguinette ◽

Felipe Dutra Rêgo ◽

...

Keyword(s):

Urban Areas ◽

Molecular Detection ◽

Minas Gerais ◽

Sand Fly ◽

Leishmania Braziliensis ◽

Sand Flies ◽

Light Traps ◽

Endemic Focus ◽

Belo Horizonte ◽

The Relationship

Abstract Sand flies are often collected in urban areas, which has several implications for the risk of transmission of Leishmania Ross, 1903, to humans and other mammals. Given this scenario, we describe the sand fly fauna of caves and their surroundings in Mangabeiras Municipal Park (MMP) and Paredão Serra do Curral Park (PSCP), both located in the urban area of Belo Horizonte, Minas Gerais, Brazil, an endemic focus of visceral and cutaneous leishmaniasis. Collections were conducted monthly from November 2011 to October 2012, using CDC light traps exposed for two consecutive nights in four caves and their surroundings. Nonsystematized collections using Shannon traps and active searches were also performed around the caves. The presence of Leishmania DNA in collected female sand flies was evaluated by ITS1-PCR. A total of 857 sand flies representing fourteen species were collected in MMP, of which Evandromyia edwardsi (Mangabeira, 1941) was the most abundant. Leishmania amazonensis was detected in Brumptomyia nitzulescui (Costa Lima, 1932) and Ev. edwardsi, with the latter also having Leishmania braziliensis, Leishmania infantum, and Leishmania sp. A total of 228 sand flies representing four species were collected in PSCP, of which Sciopemyia microps (Mangabeira, 1942) was the most abundant. No females from PSCP were positive for Leishmania-DNA. Studies aimed at describing sand fly faunas of cave environments and detecting Leishmania are essential to understanding the relationship between these insects and this ecotope and assessing and monitoring areas that may pose risks to the health of visitors and employees.

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Management of minor ailments in primary schoolchildren in rural and urban areas

Child Care Health and Development ◽

10.1046/j.1365-2214.1996.787787.x ◽

1996 ◽

Vol 22 (3) ◽

pp. 167-174

Author(s):

J A Cantrill ◽

B Johannesson ◽

M Nicholson ◽

P R Noyce

Keyword(s):

Urban Areas ◽

Rural And Urban Areas ◽

Rural And Urban ◽

Primary Schoolchildren ◽

Minor Ailments

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Cannabis use in Switzerland: The role of attribution of drug use to friends, urbanization and repression 1The study was supported by the Swiss Federal Office of Public Health (Bundesamt für Gesundheit BAG) Grant 316.96.5980.

Swiss Journal of Psychology ◽

10.1024//1421-0185.60.2.99 ◽

2001 ◽

Vol 60 (2) ◽

pp. 99-107 ◽

Cited By ~ 7

Author(s):

Holger Schmid

Keyword(s):

Drug Use ◽

Urban Areas ◽

Hierarchical Linear Model ◽

Cannabis Use ◽

School Aged Children ◽

Structural Variables ◽

Hbsc Study ◽

Nationally Representative ◽

Highly Correlated ◽

Individual Use

Cannabis use does not show homogeneous patterns in a country. In particular, urbanization appears to influence prevalence rates, with higher rates in urban areas. A hierarchical linear model (HLM) was employed to analyze these structural influences on individuals in Switzerland. Data for this analysis were taken from the Switzerland survey of Health Behavior in School-Aged Children (HBSC) Study, the most recent survey to assess drug use in a nationally representative sample of 3473 15-year-olds. A total of 1487 male and 1620 female students indicated their cannabis use and their attributions of drug use to friends. As second level variables we included address density in the 26 Swiss Cantons as an indicator of urbanization and officially recorded offences of cannabis use in the Cantons as an indicator of repressive policy. Attribution of drug use to friends is highly correlated with cannabis use. The correlation is even more pronounced in urban Cantons. However, no association between recorded offences and cannabis use was found. The results suggest that structural variables influence individuals. Living in an urban area effects the attribution of drug use to friends. On the other hand repressive policy does not affect individual use.

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