scholarly journals Direct enzyme-linked aptamer assay (DELAA) for diagnosis of toxoplasmosis by detection of SAG1 protein in mice and humans

Acta Tropica ◽  
2021 ◽  
pp. 106255
Author(s):  
Wen Cui ◽  
Xuhang Shen ◽  
Cong Wang ◽  
Asma Bibi ◽  
Obed Cudjoe ◽  
...  
Keyword(s):  
Aptamer Assay

scholarly journals A Simple Displacement Aptamer Assay on Resistive Pulse Sensor for Small Molecule Detection

2020 ◽  
Author(s):  
Rhushabh Maugi ◽  
bernadette gamble ◽  
david bunka ◽  
Mark Platt
Keyword(s):  
Small Molecules ◽  
Small Molecule ◽  
Label Free ◽  
Resistive Pulse ◽  
Pulse Sensor ◽  
Label Free Detection ◽  
Ssdna Aptamer ◽  
Aptamer Assay ◽  
Small Molecule Detection ◽  
Surface Changes

A universal aptamer-based sensing strategy is proposed using DNA modified nanocarriers and Resistive Pulse Sensing for the rapid and label free detection of small molecules. The surface of a magnetic nanocarrier was first modified with a ssDNA aka linker which is designed to be partially complimentary in sequence to a ssDNA aptamer. The aptamer and linker form a stable dsDNA complex on the nanocarriers surface. Upon the addition of the target molecule, a conformational change takes place where the aptamer preferentially binds to the target over the linker; causing the aptamer to be released into solution. The RPS measures the change in velocity of the nanocarrier as its surface changes from dsDNA to ssDNA, and its velocity is used as a proxy for the concentration of the target. We illustrate the versatility of the assay by demonstrating the detection of the antibiotic Moxifloxacin, and chemotherapeutics Imatinib and Irinotecan.

scholarly journals Detection of ochratoxin A in aptamer assay using total internal reflection ellipsometry

2018 ◽  
Vol 263 ◽  
pp. 248-251 ◽  
Author(s):  
Ali Al Rubaye ◽  
Alexei Nabok ◽  
Gaelle Catanante ◽  
Jean-Louis Marty ◽  
Ezster Takacs ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Total Internal Reflection ◽  
Internal Reflection ◽  
Aptamer Assay ◽  
Total Internal Reflection Ellipsometry

High-Throughput Bioanalysis of Bevacizumab in Human Plasma Based on Enzyme-Linked Aptamer Assay Using Anti-Idiotype DNA Aptamer

2019 ◽  
Vol 91 (4) ◽  
pp. 3125-3130 ◽  
Author(s):  
Tomohiro Yamada ◽  
Taro Saito ◽  
Yoshia Hill ◽  
Yutaka Shimizu ◽  
Kaori Tsukakoshi ◽  
...  
Keyword(s):  
Human Plasma ◽  
High Throughput ◽  
Dna Aptamer ◽  
Aptamer Assay

Development of non-equilibrium rapid replacement aptamer assay for ultra-fast detection of phthalic acid esters

Talanta ◽  
2020 ◽  
Vol 219 ◽  
pp. 121216
Author(s):  
Dabin Kim ◽  
Hyun Jeong Lim ◽  
Yun Gyong Ahn ◽  
Beelee Chua ◽  
Ahjeong Son
Keyword(s):  
Phthalic Acid ◽  
Phthalic Acid Esters ◽  
Fast Detection ◽  
Aptamer Assay ◽  
Rapid Replacement ◽  
Acid Esters ◽  
Non Equilibrium

Application of Gold Nanoparticles for High-Sensitivity Fluorescence Polarization Aptamer Assay for Ochratoxin A

2019 ◽  
Vol 14 (7-8) ◽  
pp. 397-404 ◽  
Author(s):  
A. V. Samokhvalov ◽  
I. V. Safenkova ◽  
S. A. Eremin ◽  
A. V. Zherdev ◽  
B. B. Dzantiev
Keyword(s):  
Gold Nanoparticles ◽  
Ochratoxin A ◽  
Fluorescence Polarization ◽  
High Sensitivity ◽  
Aptamer Assay

scholarly journals Enzyme-Linked Aptamer Assay (ELAA) for Detection of Toxoplasma ROP18 Protein in Human Serum

2019 ◽  
Vol 9 ◽  
Author(s):  
Monica Vargas-Montes ◽  
Nestor Cardona ◽  
Diego Mauricio Moncada ◽  
Diego Alejandro Molina ◽  
Yang Zhang ◽  
...  
Keyword(s):  
Human Serum ◽  
Aptamer Assay

Determination of the platelet-derived growth factor BB by a sandwich format thrombin-linked aptamer assay on a microplate

2019 ◽  
Vol 11 (28) ◽  
pp. 3532-3537 ◽  
Author(s):  
Limin Guo
Keyword(s):  
Growth Factor ◽  
High Throughput ◽  
Rapid Detection ◽  
Platelet Derived Growth Factor ◽  
High Throughput Analysis ◽  
Throughput Analysis ◽  
Aptamer Assay

Microplate based assays have advantages such as rapid detection and high throughput analysis.

scholarly journals Sensitivity and Selectivity on Aptamer-Based Assay: The Determination of Tetracycline Residue in Bovine Milk

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Sohee Jeong ◽  
Insook Rhee Paeng
Keyword(s):  
Dynamic Range ◽  
Bovine Milk ◽  
Point Of View ◽  
Dna Aptamer ◽  
Binding Assays ◽  
Good Tool ◽  
Sensitivity And Selectivity ◽  
Data Points ◽  
Aptamer Assay

A competitive enzyme-linked aptamer assay (ELAA) to detect tetracycline in milk was performed by using two different aptamers individually; one is 76 mer-DNA aptamer and the other is 57 mer-RNA aptamer. The best optimum condition was obtained without monovalent ion, Na+and also by adding no Mg2+ion in the assay buffer, along with RT incubation. The optimized ELAA showed a good sensitivity (LOD of 2.10 × 10−8M) with a wide dynamic range (3.16 × 10−8M ~ 3.16 × 10−4M). In addition, the average R.S.D. across all data points of the curve was less than 2.5% with good recoveries (~101.8%) from the milk media. Thus, this method provides a good tool to monitor tetracycline in milk from MRLs’ point of view. However, this ELAA method was not superior to the ELISA method in terms of specificity. This paper describes that it does not always give better sensitivity and specificity in assays even though aptamers have several advantages over antibodies and have been known to be good binders for binding assays.

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