Stem cell factor suppressed IL-33-induced MHC class II expression in murine bone marrow-derived mast cells

We investigated the effects of stem cell factor (SCF) on the migration of murine bone marrow hematopoietic progenitor cells (HPC) in vitro using a modification of the checkerboard assay. Chemotactic and chemokinetic activities of SCF on HPC were evaluated by the numbers of HPC migrated on positive and negative gradients of SCF, respectively. On both positive and negative gradients of SCF, HPC began to migrate after 4 hours incubation, and their numbers then increased time- dependently. These results indicated that SCF functions as a chemotactic and chemokinetic agent for HPC. Analysis of types of colonies derived from the migrated HPC showed that SCF had chemotactic and chemokinetic effects on all types of HPC. When migrating activities of other cytokines were examined, interleukin (IL)-3 and IL-11 also affected the migration of HPC, but the degrees of each effect were lower than that of SCF. The results of the present study demonstrated that SCF is one of the most potent chemotactic and chemokinetic factors for HPC and suggest that SCF may play an important role in the flow of HPC into bone marrow where stromal cells constitutively produce SCF.

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Characterization of ‘adult-type’ mast cells derived from human bone marrow CD34+ cells cultured in the presence of stem cell factor and interleukin-6. Interleukin-4 is not required for constitutive expression of CD54, FcεRIα and chymase, and CD13 expressi

Clinical & Experimental Allergy ◽

10.1046/j.1365-2222.2002.01373.x ◽

2002 ◽

Vol 32 (6) ◽

pp. 872-880 ◽

Cited By ~ 34

Author(s):

Y. Shimizu ◽

K. Sakai ◽

T. Miura ◽

T. Narita ◽

H. Tsukagoshi ◽

...

Keyword(s):

Bone Marrow ◽

Stem Cell ◽

Mast Cells ◽

Stem Cell Factor ◽

Constitutive Expression ◽

Interleukin 4 ◽

Adult Type ◽

Cd34 Cells ◽

Cells Cultured

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Membrane-Bound Human Stem Cell Factor Promotes Differentiation of Human Granulocytes and Mast Cells In Vivo,

Blood ◽

10.1182/blood.v118.21.3419.3419 ◽

2011 ◽

Vol 118 (21) ◽

pp. 3419-3419

Author(s):

Shinsuke Takagi ◽

Yoriko Saito ◽

Atsushi Hijikata ◽

Satoshi Tanaka ◽

Takashi Watanabe ◽

...

Keyword(s):

Bone Marrow ◽

Stem Cell ◽

Mast Cells ◽

Stem Cell Factor ◽

Myeloid Cells ◽

Human Stem Cell ◽

Myeloid Development ◽

Membrane Bound ◽

Human Stem Cell Factor

Abstract Abstract 3419 Recently, advances in xenograft models for human hemamtopoietic stem cells (HSCs), or the humanized mice, have begun to allow investigators to examine the differentiation of human hematopoietic and immune cells in vivo. However, lymphoid-skewed human hematopoietic development in the mouse bone marrow is one of the remaining limitations in the humanized mouse models. The inefficient human myeloid development could at least partly be attributed to the mouse microenvironment not fully supporting differentiation and maturation of human myeloid lineage. To overcome this problem, we focused on the role of membrane-bound human stem cell factor in supporting the maintenance of human HSCs and inducing the development of human myeloid cells and created human stem cell factor transgenic NOD/SCID/IL2rgKO (hSCF Tg NSG) mice. Transplantation of 5000–50000 cord blood-derived Lin-CD34+CD38- cells resulted in significantly higher engraftment of human CD45+ leukocytes at 3–6 months post-transplantation in the bone marrow, spleen, and peripheral blood of hSCF Tg NSG recipients compared with those of non-transgenic NSG recipients. The enhanced human CD45+ engraftment was most prominent in the bone marrow (hSCF Tg recipients: 98.0 +/− 1.3%, n= 15, non-Tg NSG controls: 75.3 +/− 7.3%, n=7). In the bone marrow, the frequency of human CD33+ myeloid cells within the total human CD45+ population was significantly higher in the hSCF Tg NSG recipients than in the non-Tg NSG recipients and constituted the majority of human hematopoietic cells (hSCF Tg recipients: 54.6 +/− 4.5%, n=15 and non-Tg NSG controls: 29.3 +/− 4.0%, n=7). Flow cytometric analysis demonstrated that the majority of engrafted human myeloid cells in the hSCF Tg recipient bone marrow were side-scatter high, HLA-DR negative granulocytes. Reflecting the effect of human SCF on the development of human mast cells, human c-Kit+CD203c+ mast cells were identified in the bone marrow, spleen, and gastrointestinal tracts of the hSCF Tg NSG recipients. Altogether, the in vivo humanized mouse model demonstrates the essential role of membrane-bound SCF in human myeloid development. The hSCF Tg NSG humanized mice may facilitate the in vivo investigation of human HSCs, myeloid progenitors and mature myeloid lineage. Disclosures: No relevant conflicts of interest to declare.

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The effects of recombinant ovine interleukin-3 and recombinant ovine stem cell factor on the growth and mediator expression of caprine and ovine bone marrow-derived mast cells

Veterinary Immunology and Immunopathology ◽

10.1016/s0165-2427(97)00086-x ◽

1997 ◽

Vol 60 (1-2) ◽

pp. 97-110 ◽

Cited By ~ 4

Author(s):

Colin N. Macaldowie ◽

John F. Huntley ◽

Anne Mackellar ◽

Colin McInnes ◽

David M. Haig

Keyword(s):

Bone Marrow ◽

Stem Cell ◽

Mast Cells ◽

Stem Cell Factor ◽

Interleukin 3

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Combined Effects of Interleukin-7 and Stem Cell Factor Administration on Lymphopoiesis after Murine Bone Marrow Transplantation

Biology of Blood and Marrow Transplantation ◽

10.1016/j.bbmt.2010.07.027 ◽

2011 ◽

Vol 17 (1) ◽

pp. 48-60 ◽

Cited By ~ 11

Author(s):

Brile Chung ◽

Dullei Min ◽

Lukas W. Joo ◽

Mark R. Krampf ◽

Jing Huang ◽

...

Keyword(s):

Bone Marrow ◽

Stem Cell ◽

Bone Marrow Transplantation ◽

Marrow Transplantation ◽

Stem Cell Factor ◽

Combined Effects ◽

Murine Bone Marrow ◽

Interleukin 7

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Effects of stem cell factor (kit-ligand) and interleukin-3 on the growth and serine proteinase expression of rat bone-marrow-derived or serosal mast cells

Blood ◽

10.1182/blood.v83.1.72.72 ◽

1994 ◽

Vol 83 (1) ◽

pp. 72-83 ◽

Cited By ~ 41

Author(s):

DM Haig ◽

JF Huntley ◽

A MacKellar ◽

GF Newlands ◽

L Inglis ◽

...

Keyword(s):

Bone Marrow ◽

Stem Cell ◽

Mast Cells ◽

Stem Cell Factor ◽

Synergistic Effects ◽

Serine Proteinase ◽

In Vitro Growth ◽

Interleukin 3 ◽

Kit Ligand

Abstract The effects of rat stem-cell factor (SCF) and interleukin-3 (IL-3), alone or in combination, on the in vitro growth and serine proteinase expression of rat serosal/connective-tissue mast cells (CTMC) or bone marrow-derived mast cells (BMMC) were examined. Rat SCF stimulated the growth of both CTMC and BMMC. IL-3 stimulated BMMC growth to a lesser extent than did SCF, whereas CTMC numbers did not increase in IL-3. However, SCF and IL-3 had synergistic effects on the growth of both BMMC and CTMC. SCF favoured the maintenance of rat mast cell proteinase- I (RMCP-I) in CTMC, but did not induce detectable production of RMCP-I in BMMC. In contrast, when IL-3 or lymph node-conditioned medium (LNCM) was added to SCF, a subpopulation of CTMC expressed and stored the soluble proteinase RMCP-II. In BMMC, the RMCP-II content of cells maintained in SCF was significantly less than that of cells maintained in IL-3 or LNCM. RMCP-II also appeared in the supernatants of BMMC, especially when BMMC numbers were increasing rapidly in SCF with or without IL-3 or LNCM. Thus, SCF and IL-3 can regulate the growth of rat BMMC and CTMC, as well as influence their production and release of proteinases.

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