A two-dimensional inverse analysis utilizes a different perspective to simultaneously estimate the center and surface thermal behavior of a heated cylinder normal to a turbulent air stream. A finite-difference method is used to discretize the governing equations and then a linear inverse model is constructed to identify the unknown boundary conditions. The present approach is to rearrange the matrix forms of the governing differential equations and estimate the unknown boundary conditions of the heated cylinder. Then, the linear least-squares-error method is adopted to find the solutions. The results show that only a few measuring points inside the cylinder are needed to estimate the unknown quantities of the thermal boundary behavior, even when measurement errors are considered. In contrast to the traditional approach, the advantages of this method are that no prior information is needed on the functional form of the unknown quantities, no initial guesses are required, no iterations in the calculating process are necessary, and the inverse problem can be solved in a linear domain. Furthermore, the existence and uniqueness of the solutions can easily be identified.