High-throughput 16S rRNA gene sequencing reveals alterations of mouse intestinal microbiota after radiotherapy

Abstract BackgroudWeifuchun(WFC), a Chinese herbal prescription consisting of Red Ginseng, Isodon amethystoides and Fructus Aurantii, is commonly used in China to treat a variety of chronic stomach disorders. The aim of the paper is to determine the effect of WFC on intestinal microbiota changes in precancerous lesions of gastric cancer (PLGC) patients. MethodsPLGC patients of H.pylori negative were randomly divided into two groups received either WFC tablets at a dose of 1.44g 3 times a day or vitacoenzyme tablets at a dose of 0.8g 3 times a day. All patients were treated for 6 months, consecutively. Gastroscopy and histopathology were used to assess the histopathological changes in the gastric tissues before and after treatment. 16S rRNA gene sequencing was carried out to assess the effects WFC on intestinal microbiota changes in PLGC patients. Receiver Operating Characteristics(ROC) analysis was used to assess the sensitivity and specificity of different intestinal microbiota in distinguishing between PLGC patients and healthy control. ResultsGastroscopy and histopathological results indicated that WFC could improve the pathological condition of PLGC patients, especially in the atrophy or intestinal metaplasia. The results of 16S rRNA gene sequencing indicated that WFC could regulate the microbial diversity, microbial composition, and abundance of the intestinal microbiota of PLGC patients. Following WFC treatment, the relative abundance of Parabacteroides was decreased in WFC group when compared with the control group. ROC analysis found that the Parabacteroides could effectively distinguish PLGC patients from healthy individuals with sensitivity of 0.79 and specificity of 0.8. ConclusionsWFC could slow down the progression of PLGC by regulating intestinal microbiota abundance. Trial registrationNCT03814629. Name of registry: Randomized Clinical Trial: Weifuchun Treatment on Precancerous Lesions of Gastric Cancer. Registered 3 August 2018-Retrospectively registered, https://register.clinicaltrials.gov/ NCT03814629.

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Ontogenetic Characterization of the Intestinal Microbiota of Channel Catfish through 16S rRNA Gene Sequencing Reveals Insights on Temporal Shifts and the Influence of Environmental Microbes

PLoS ONE ◽

10.1371/journal.pone.0166379 ◽

2016 ◽

Vol 11 (11) ◽

pp. e0166379 ◽

Cited By ~ 42

Author(s):

Jacob W. Bledsoe ◽

Brian C. Peterson ◽

Kelly S. Swanson ◽

Brian C. Small

Keyword(s):

16S Rrna ◽

16S Rrna Gene ◽

Intestinal Microbiota ◽

Channel Catfish ◽

Gene Sequencing ◽

16S Rrna Gene Sequencing ◽

Rrna Gene ◽

Rrna Gene Sequencing ◽

Environmental Microbes

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Analysis of the intestinal microbiota using SOLiD 16S rRNA gene sequencing and SOLiD shotgun sequencing

BMC Genomics ◽

10.1186/1471-2164-14-s5-s16 ◽

2013 ◽

Vol 14 (Suppl 5) ◽

pp. S16 ◽

Cited By ~ 23

Author(s):

Suparna Mitra ◽

Karin Förster-Fromme ◽

Antje Damms-Machado ◽

Tim Scheurenbrand ◽

Saskia Biskup ◽

...

Keyword(s):

16S Rrna ◽

16S Rrna Gene ◽

Intestinal Microbiota ◽

Gene Sequencing ◽

16S Rrna Gene Sequencing ◽

Shotgun Sequencing ◽

Rrna Gene ◽

Rrna Gene Sequencing

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Effects of probiotics and prebiotics on intestinal microbiota in mice with acute colitis based on 16S rRNA gene sequencing

Chinese Medical Journal ◽

10.1097/cm9.0000000000000308 ◽

2019 ◽

Vol 132 (15) ◽

pp. 1833-1842 ◽

Cited By ~ 7

Author(s):

Ya-Nan Wang ◽

Xiang-Chen Meng ◽

Yi-Fan Dong ◽

Xin-Hua Zhao ◽

Jia-Ming Qian ◽

...

Keyword(s):

16S Rrna ◽

16S Rrna Gene ◽

Intestinal Microbiota ◽

Gene Sequencing ◽

16S Rrna Gene Sequencing ◽

Rrna Gene ◽

Acute Colitis ◽

Rrna Gene Sequencing

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A correlational study of Weifuchun and its clinical effect on intestinal flora in precancerous lesions of gastric cancer

Chinese Medicine ◽

10.1186/s13020-021-00529-9 ◽

2021 ◽

Vol 16 (1) ◽

Author(s):

Yanqin Bian ◽

Xi Chen ◽

Hongyan Cao ◽

Dong Xie ◽

Meiping Zhu ◽

...

Keyword(s):

Gastric Cancer ◽

16S Rrna ◽

16S Rrna Gene ◽

Intestinal Microbiota ◽

Roc Analysis ◽

Precancerous Lesions ◽

Gene Sequencing ◽

16S Rrna Gene Sequencing ◽

Rrna Gene ◽

Rrna Gene Sequencing

Abstract Background Weifuchun (WFC), a Chinese herbal prescription consisting of Red Ginseng, Isodon amethystoides and Fructus Aurantii, is commonly used in China to treat a variety of chronic stomach disorders. The aim of the paper was to determine the effect of WFC on intestinal microbiota changes in precancerous lesions of gastric cancer (PLGC) patients. Methods PLGC patients of H. pylori negative were randomly divided into two groups and received either WFC tablets for a dose of 1.44 g three times a day or vitacoenzyme (Vit) tablets for a dose of 0.8 g three times a day. All patients were treated for 6 months consecutively. Gastroscopy and histopathology were used to assess the histopathological changes in gastric tissues before and after treatment. 16S rRNA gene sequencing was carried out to assess the effects WFC on intestinal microbiota changes in PLGC patients. Receiver Operating Characteristics (ROC) analysis was used to assess the sensitivity and specificity of different intestinal microbiota in distinguishing between PLGC patients and healthy control group. Results Gastroscopy and histopathological results indicated that WFC could improve the pathological condition of PLGC patients, especially in the case of atrophy or intestinal metaplasia. The results of 16S rRNA gene sequencing indicated that WFC could regulate microbial diversity, microbial composition, and abundance of the intestinal microbiota of PLGC patients. Following WFC treatment, the relative abundance of Parabacteroides decreased in WFC group when compared with the Vit group. ROC analysis found that the Parabacteroides could effectively distinguish PLGC patients from healthy individuals with sensitivity of 0.79 and specificity of 0.8. Conclusions WFC could slow down the progression of PLGC by regulating intestinal microbiota abundance. Trial registration NCT03814629. Name of registry: Randomized Clinical Trial: Weifuchun Treatment on Precancerous Lesions of Gastric Cancer. Registered 3 August 2018-Retrospectively registered, https://register.clinicaltrials.gov/ NCT03814629.

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