scholarly journals Spermatozoa in the sperm-peak-fraction of the boar ejaculate show a lower flow of Ca2+ under capacitation conditions post-thaw which might account for their higher membrane stability after cryopreservation

2011 ◽  
Vol 128 (1-4) ◽  
pp. 37-44 ◽  
Author(s):  
Md. Sharoare Hossain ◽  
Anders Johannisson ◽  
Amanda Pimenta Siqueira ◽  
Margareta Wallgren ◽  
Heriberto Rodriguez-Martinez
Blood ◽  
1983 ◽  
Vol 61 (2) ◽  
pp. 373-377 ◽  
Author(s):  
JE Smith ◽  
K Moore ◽  
M Arens ◽  
GA Rinderknecht ◽  
A Ledet

Abstract A dog with persistent elliptocytosis was studied. The dog had membrane protein band 4.1 deficiency, microcytosis, shortened erythrocyte lifespan, increased osmotic sensitivity, and a mild glutathione deficiency. Erythrocyte deformability and membrane stability were adversely effected. The dog's parents had decreased band 4.1, decreased stability, and some elliptocytosis. This disorder in dogs closely resembles human patients with band 4.1 deficiency and should provide a valuable animal model to study the erythrocyte membrane cytoskeleton.


2021 ◽  
Vol 6 (4) ◽  
Author(s):  
Christiana Mavroyiakoumou ◽  
Silas Alben

Immunobiology ◽  
2016 ◽  
Vol 221 (10) ◽  
pp. 1181
Author(s):  
Niv Mazkereth ◽  
Francesco Rocca ◽  
Jennifer-Rose Schubert ◽  
Claudia Geisler ◽  
Yaron Hillman ◽  
...  

Author(s):  
Yangyang Wu ◽  
Xin Zhang ◽  
Xi Zhang ◽  
Siyu Liu ◽  
Jintao Zhang ◽  
...  

Abstract Sperm is the ultimate executor of male reproductive function. Normal morphology, quantity, and motility of sperm ensure the normal reproductive process. Palmitoylation is a posttranslational modification mediated by palmitoyltransferases whereby palmitoyl is added to proteins. Seven palmitoyltransferases have been identified in Saccharomyces cerevisiae and 23 in humans (including ZDHHC1–9 and ZDHHC11–24), with corresponding homologs in mice. We identified two testis-specific palmitoyltransferases ZDHHC11 and ZDHHC19 in mice. The Zdhhc11 and Zdhhc19-knockout mouse models were constructed, and it was found that the Zdhhc11 knockout males were fertile, while Zdhhc19 knockout males were sterile. ZDHHC19 is located in the cell membrane of step 4–9 spermatids in the mouse testis, and phenotypic analysis showed that the testicular weight ratio in the Zdhhc19−/− mice decreased along with the number and motility of the sperm decreased, while sperm abnormalities increased, mainly due to the “folded” abnormal sperm caused by sperm membrane fusion, suggesting the involvement of ZDHHC19 in maintaining membrane stability in the male reproductive system. In addition, Zdhhc19−/− mice showed abnormal sperm morphologies and apoptosis during spermatogenesis, suggesting that spermatogenesis in the Zdhhc19−/− mice was abnormal. These results indicate that ZDHHC19 promotes membrane stability in male germ cells. Summary sentence: ZDHHC19 is located in the cell membrane of Step4–9 spermatids in mouse testis; Zdhhc19 knockout mice showed male infertility, abnormal spermatogenesis, sperm morphology and motility.


2015 ◽  
Vol 21 ◽  
pp. 212-220 ◽  
Author(s):  
Kraiwith Wongkaew ◽  
Ura Pancharoen ◽  
Suphot Phatanasri ◽  
Natchanun Leepipatpiboon ◽  
Anchaleeporn W. Lothongkum
Keyword(s):  

2017 ◽  
Vol 45 (1) ◽  
pp. 82-88
Author(s):  
Mahnaz ZAFARI ◽  
Ali EBADI ◽  
Sodabeh JAHANBAKHSH GODEHKAHRIZ

Water deficit is one of the most important environmental stresses that adversely affect crop growth and production and mycorrhizal fungi and symbiotic bacteria have important role in resistance to drought stress. The effect of biofertilizers on alfalfa stress tolerance was studied at the greenhouse condition. Treatments comprised three water-deficit stresses (35%, 55% and 75% of field capacity) and four seeds inoculations (Glomus mosseae, Sinorhizobium meliloti, G. mosseae + S. meliloti and non-inoculated). Water-deficit stress decrease cell membrane stability (39%), total Chl (24.05%), carotenoid (35.55%), quantum yield (50.64%) and forage yield (28.20%), while increased the proline and soluble sugars content (68.55 and 46.53% respectively) and osmotic potential (45.84%). The inoculation of seeds increased the capability of the plants in counteracting the stress, so that the production of compatible solutes was increased and the photosynthetic indices, proline, osmotic potential, membrane stability and forage yield were improved by seed inoculation. Mycorrhiza improved photosynthetic indexes and proline, but bacteria had more efficacy on membrane stability and forage yield. However, double inoculation due to the synergistic effect of mycorrhiza and Sinorhizobium, had the greatest effect than Solitary inoculation. Our results suggest that biofertilized alfalfa plants were better adapted than non- biofertilized ones to cope with water deficit.


2002 ◽  
Vol 459 ◽  
pp. 317-345 ◽  
Author(s):  
Y. BERTHO ◽  
F. GIORGIUTTI-DAUPHINÉ ◽  
T. RAAFAT ◽  
E. J. HINCH ◽  
H. J. HERRMANN ◽  
...  

The dynamics of dry granular flows down a vertical glass pipe of small diameter have been studied experimentally. Simultaneous measurements of pressure profiles, air and grain flow rates and volume fractions of particles have been realized together with spatio-temporal diagrams of the grain distribution down the tube. At large grain flow rates, one observes a stationary flow characterized by high particle velocities, low particle fractions and a downflow of air resulting in an underpressure in the upper part of the pipe. A simple model assuming a free fall of the particles slowed down by air friction and taking into account finite particle fraction effects through Richardson–Zaki's law has been developed: it reproduces pressure and particle fraction variations with distance and estimates friction forces with the wall. At lower flow rates, sequences of high-density plugs separated by low-density bubbles moving down at a constant velocity are observed. The pressure is larger than outside the tube and its gradient reflects closely the weight of the grains. Writing mass and momentum conservation equations for the air and for the grains allows one to estimate the wall friction, which is less than 10% of the weight for grains with a clean smooth surface but up to 30% for grains with a rougher surface. At lower flow rates, oscillating-wave regimes resulting in large pressure fluctuations are observed and their frequency is predicted.


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