In vitro and in vivo studies of three dimensional porous composites of biphasic calcium phosphate/poly ɛ-caprolactone: Effect of bio-functionalization for bone tissue engineering

2014 ◽  
Vol 301 ◽  
pp. 307-314 ◽  
Author(s):  
Kyung-A. Kwak ◽  
Md. Anirban Jyoti ◽  
Ho-Yeon Song
Keyword(s):  
Tissue Engineering ◽  
Calcium Phosphate ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Biphasic Calcium Phosphate ◽  
Three Dimensional ◽  
In Vivo Studies ◽  
Porous Composites

scholarly journals Multifunctional 3D-Printed Magnetic Polycaprolactone/Hydroxyapatite Scaffolds for Bone Tissue Engineering

Polymers ◽  
2021 ◽  
Vol 13 (21) ◽  
pp. 3825
Author(s):  
Mauro Petretta ◽  
Alessandro Gambardella ◽  
Giovanna Desando ◽  
Carola Cavallo ◽  
Isabella Bartolotti ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Superparamagnetic Iron Oxide ◽  
In Vivo Studies ◽  
Osteogenic Potential ◽  
Great Promise ◽  
Good Cell

Multifunctional and resistant 3D structures represent a great promise and a great challenge in bone tissue engineering. This study addresses this problem by employing polycaprolactone (PCL)-based scaffolds added with hydroxyapatite (HAp) and superparamagnetic iron oxide nanoparticles (SPION), able to drive on demand the necessary cells and other bioagents for a high healing efficiency. PCL-HAp-SPION scaffolds with different concentrations of the superparamagnetic component were developed through the 3D-printing technology and the specific topographical features were detected by Atomic Force and Magnetic Force Microscopy (AFM-MFM). AFM-MFM measurements confirmed a homogenous distribution of HAp and SPION throughout the surface. The magnetically assisted seeding of cells in the scaffold resulted most efficient for the 1% SPION concentration, providing good cell entrapment and adhesion rates. Mesenchymal Stromal Cells (MSCs) seeded onto PCL-HAp-1% SPION showed a good cell proliferation and intrinsic osteogenic potential, indicating no toxic effects of the employed scaffold materials. The performed characterizations and the collected set of data point on the inherent osteogenic potential of the newly developed PCL-HAp-1% SPION scaffolds, endorsing them towards next steps of in vitro and in vivo studies and validations.

scholarly journals In vitro and in vivo studies of a gelatin/carboxymethyl chitosan/LAPONITE® composite scaffold for bone tissue engineering

RSC Advances ◽  
2017 ◽  
Vol 7 (85) ◽  
pp. 54100-54110 ◽  
Author(s):  
Li Tao ◽  
Liu Zhonglong ◽  
Xiao Ming ◽  
Yang Zezheng ◽  
Liu Zhiyuan ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Freeze Drying ◽  
Composite Scaffold ◽  
Carboxymethyl Chitosan ◽  
In Vivo Studies ◽  
Potential Use

In the present study, we fabricated a biocomposite scaffold composed of carboxymethyl chitosan (CMC), gelatin and LAPONITE® (Lap) nanoparticles via freeze-drying and investigated its potential use in bone tissue engineering.

Towards Engineering Whole Bones via Endochondral Ossification

2013 ◽  
Author(s):  
Eamon J. Sheehy ◽  
Tatiana Vinardell ◽  
Conor T. Buckley ◽  
Daniel J. Kelly
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Three Dimensional ◽  
Low Oxygen ◽  
Engineering Applications ◽  
Endochondral Bone ◽  
Oxygen Conditions

Tissue engineering applications aim to replace or regenerate damaged tissues through a combination of cells, three-dimensional scaffolds, and signaling molecules [1]. The endochondral approach to bone tissue engineering [2], which involves remodeling of an intermittent hypertrophic cartilaginous template, may be superior to the traditional intramembranous approach. Naturally derived hydrogels have been used extensively in tissue engineering applications [3]. Mesenchymal stem cell (MSC) seeded hydrogels may be a particularly powerful tool in scaling-up engineered endochondral bone grafts as the low oxygen conditions that develop within large constructs enhance in vitro chondrogenic differentiation and functional development [4]. A key requirement however, is that the hydrogel must allow for remodeling of the engineered hypertrophic cartilage into bone and also facilitate vascularization of the graft. The first objective of this study was to compare the capacity of different naturally derived hydrogels (alginate, chitosan, and fibrin) to generate in vivo endochondral bone. The secondary objective was to investigate the possibility of engineering a ‘scaled-up’ anatomically accurate distal phalange as a paradigm for whole bone tissue engineering.

scholarly journals Evaluation of Strontium-Containing PCL-PDIPF Scaffolds for Bone Tissue Engineering: In Vitro and In Vivo Studies

2018 ◽  
Vol 47 (3) ◽  
pp. 902-912 ◽  
Author(s):  
Agustina Berenice Lino ◽  
Antonio Desmond McCarthy ◽  
Juan Manuel Fernández
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
In Vivo Studies

Poly(lactic-co-glycolic acid)(PLGA)/TiO 2 nanotube bioactive composite as a novel scaffold for bone tissue engineering: In vitro and in vivo studies

Biologicals ◽  
2018 ◽  
Vol 53 ◽  
pp. 51-62 ◽  
Author(s):  
Hossein Eslami ◽  
Hamidreza Azimi Lisar ◽  
Tahereh Sadat Jafarzadeh Kashi ◽  
Mohammadreza Tahriri ◽  
Mojtaba Ansari ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Glycolic Acid ◽  
In Vivo Studies ◽  
Novel Scaffold

In-vitro evaluation of biphasic calcium phosphate/casein incorporated with Myristica fragrans for bone tissue engineering

2015 ◽  
Vol 41 (1) ◽  
pp. 1725-1734 ◽  
Author(s):  
B. Santhosh Kumar ◽  
T. Muthukumar ◽  
R. Deepachitra ◽  
R.K. Charumathy ◽  
T. Hemalatha ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Calcium Phosphate ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Biphasic Calcium Phosphate ◽  
In Vitro Evaluation ◽  
Myristica Fragrans
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