In-vitro evaluation of biphasic calcium phosphate/casein incorporated with Myristica fragrans for bone tissue engineering

AbstractNano biphasic calcium phosphate (BCP) particles were synthesized using the sol-gel method. As-prepared BCP particles were combined with poly-L-lactide (PLLA) to fabricate nano-BCP/PLLA composite scaffold through a series of processing steps containing solvent self-diffusion, hot-pressing, and particulate leaching. The composite had a suitable porous structure for bone tissue engineering scaffold. In comparison, micro-BCP/PLLA scaffold was studied as well. Nano-BCP particles were distributed homogeneously in the PLLA matrix, and much more tiny crystallites exposed on the surface of the pore wall. Due to the finer inorganic particle distribution in the PLLA phase and the larger area of the bioactive phase exposed in the pore wall surface, nano-BCP/PLLA scaffold had enhanced compressive strength, good bioactivity, and superior cell viability. A nonstoichiometric apatite layer could be rapidly formed on the surface of nano- BCP/PLLA when soaked in simulated body fluid. The MG-63 cell viability of nano-BCP/PLLA scaffold is significantly higher than that of micro-BCP/PLLA scaffold. Therefore, nano-BCP/PLLA composite may be a suitable alternative for bone tissue engineering scaffold.

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Effect of zirconia-mullite incorporated biphasic calcium phosphate/biopolymer composite scaffolds for bone tissue engineering

Biomedical Physics & Engineering Express ◽

10.1088/2057-1976/aba1c2 ◽

2020 ◽

Vol 6 (5) ◽

pp. 055004

Author(s):

Tanawut Rittidach ◽

Tanatsaparn Tithito ◽

Panan Suntornsaratoon ◽

Narattaphol Charoenphandhu ◽

Jirawan Thongbunchoo ◽

...

Keyword(s):

Tissue Engineering ◽

Calcium Phosphate ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Biphasic Calcium Phosphate ◽

Composite Scaffolds

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Fully Interconnected Globular Porous Biphasic Calcium Phosphate Ceramic Scaffold Facilitates Osteogenic Repair

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.361-363.119 ◽

2007 ◽

Vol 361-363 ◽

pp. 119-122 ◽

Cited By ~ 4

Author(s):

J.H. Lim ◽

J.H. Park ◽

Eui Kyun Park ◽

Hae Jung Kim ◽

Il Kyu Park ◽

...

Keyword(s):

Tissue Engineering ◽

Calcium Phosphate ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Bone Substitute ◽

Biphasic Calcium Phosphate ◽

Bone Defects ◽

Calcium Phosphate Ceramic ◽

Ceramic Scaffold ◽

Porous Biphasic Calcium Phosphate

An appropriate scaffold, which provides structural support for transplanted cells and acts as a vehicle for the delivery of biologically active molecules, is critical for tissue engineering. We developed a fully interconnected globular porous biphasic calcium phosphate ceramic scaffold by adopting a foaming method, and evaluated its efficiency as a bone substitute and a scaffold for bone tissue engineering by in vitro and in vivo biocompatible analysis and its osteogenic healing capacity in rat tibial bone defects. They have spherical pores averaging 400um in diameter and interconnecting interpores averaging 70um in diameter with average 85% porosity. They elicited no cytotoxicity and noxious effect on cellular proliferation and osteoblastic differentiation during the cell-scaffold construct formation. Also the bone defects grafted with fully interconnected globular porous biphasic calcium phosphate ceramic blocks revealed excellent bone healing within 3 weeks. These findings suggest that the fully interconnected porous biphasic calcium phosphate scaffold formed by the foaming method can be a promising bone substitute and a scaffold for bone tissue engineering.

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