Poly(lactic‑co‑glycolic acid)‑bioactive glass composites as nanoporous scaffolds for bone tissue engineering: In vitro and in vivo studies

Multifunctional and resistant 3D structures represent a great promise and a great challenge in bone tissue engineering. This study addresses this problem by employing polycaprolactone (PCL)-based scaffolds added with hydroxyapatite (HAp) and superparamagnetic iron oxide nanoparticles (SPION), able to drive on demand the necessary cells and other bioagents for a high healing efficiency. PCL-HAp-SPION scaffolds with different concentrations of the superparamagnetic component were developed through the 3D-printing technology and the specific topographical features were detected by Atomic Force and Magnetic Force Microscopy (AFM-MFM). AFM-MFM measurements confirmed a homogenous distribution of HAp and SPION throughout the surface. The magnetically assisted seeding of cells in the scaffold resulted most efficient for the 1% SPION concentration, providing good cell entrapment and adhesion rates. Mesenchymal Stromal Cells (MSCs) seeded onto PCL-HAp-1% SPION showed a good cell proliferation and intrinsic osteogenic potential, indicating no toxic effects of the employed scaffold materials. The performed characterizations and the collected set of data point on the inherent osteogenic potential of the newly developed PCL-HAp-1% SPION scaffolds, endorsing them towards next steps of in vitro and in vivo studies and validations.

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Silicate, borosilicate, and borate bioactive glass scaffolds with controllable degradation rate for bone tissue engineering applications. II. In vitro and in vivo biological evaluation

Journal of Biomedical Materials Research Part A ◽

10.1002/jbm.a.32823 ◽

2010 ◽

Vol 95A (1) ◽

pp. 172-179 ◽

Cited By ~ 120

Author(s):

Qiang Fu ◽

Mohamed N. Rahaman ◽

B. Sonny Bal ◽

Lynda F. Bonewald ◽

Keiichi Kuroki ◽

...

Keyword(s):

Tissue Engineering ◽

Bioactive Glass ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Degradation Rate ◽

Biological Evaluation ◽

Borate Bioactive Glass ◽

Controllable Degradation

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In vitro and in vivo studies of a gelatin/carboxymethyl chitosan/LAPONITE® composite scaffold for bone tissue engineering

RSC Advances ◽

10.1039/c7ra06913h ◽

2017 ◽

Vol 7 (85) ◽

pp. 54100-54110 ◽

Cited By ~ 23

Author(s):

Li Tao ◽

Liu Zhonglong ◽

Xiao Ming ◽

Yang Zezheng ◽

Liu Zhiyuan ◽

...

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Freeze Drying ◽

Composite Scaffold ◽

Carboxymethyl Chitosan ◽

In Vivo Studies ◽

Potential Use

In the present study, we fabricated a biocomposite scaffold composed of carboxymethyl chitosan (CMC), gelatin and LAPONITE® (Lap) nanoparticles via freeze-drying and investigated its potential use in bone tissue engineering.

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Evaluation of nano-biphasic calcium phosphate ceramics for bone tissue engineering applications: In vitro and preliminary in vivo studies

Journal of Biomaterials Applications ◽

10.1177/0885328211415132 ◽

2012 ◽

Vol 27 (5) ◽

pp. 565-575 ◽

Cited By ~ 24

Author(s):

Sujatha Reddy ◽

Samiksha Wasnik ◽

Avijit Guha ◽

Jerald Mahesh Kumar ◽

Arvind Sinha ◽

...

Keyword(s):

Tissue Engineering ◽

Calcium Phosphate ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Biphasic Calcium Phosphate ◽

In Vivo Studies ◽

Engineering Applications ◽

Calcium Phosphate Ceramics

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Evaluation of Strontium-Containing PCL-PDIPF Scaffolds for Bone Tissue Engineering: In Vitro and In Vivo Studies

Annals of Biomedical Engineering ◽

10.1007/s10439-018-02183-z ◽

2018 ◽

Vol 47 (3) ◽

pp. 902-912 ◽

Cited By ~ 6

Author(s):

Agustina Berenice Lino ◽

Antonio Desmond McCarthy ◽

Juan Manuel Fernández

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

In Vivo Studies

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Osteoconductive and electroactive carbon nanofibers/hydroxyapatite nanocomposite tailored for bone tissue engineering: in vitro and in vivo studies

Scientific Reports ◽

10.1038/s41598-020-71455-3 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Hadi Samadian ◽

Hamid Mobasheri ◽

Mahmoud Azami ◽

Reza Faridi-Majidi

Keyword(s):

Tissue Engineering ◽

Bone Formation ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Carbon Nanofibers ◽

In Vivo Studies ◽

Histological Evaluation ◽

Control Group

Abstract In this study, we aimed to fabricate osteoconductive electrospun carbon nanofibers (CNFs) decorated with hydroxyapatite (HA) crystal to be used as the bone tissue engineering scaffold in the animal model. CNFs were derived from electrospun polyacrylonitrile (PAN) nanofibers via heat treatment and the carbonized nanofibers were mineralized by a biomimetic approach. The growth of HA crystals was confirmed using XRD, FTIR, and EDAX analysis techniques. The mineralization process turned the hydrophobic CNFs (WCA: 133.5° ± 0.6°) to hydrophilic CNFs/HA nanocomposite (WCA 15.3° ± 1°). The in vitro assessments revealed that the fabricated 24M-CNFs nanocomposite was biocompatible. The osteoconductive characteristics of CNFs/HA nanocomposite promoted in vivo bone formation in the rat’s femur defect site, significantly, observed by computed tomography (CT) scan images and histological evaluation. Moreover, the histomorphometric analysis showed the highest new bone formation (61.3 ± 4.2%) in the M-CNFs treated group, which was significantly higher than the negative control group (the defect without treatment) (< 0.05). To sum up, the results implied that the fabricated CNFs/HA nanocomposite could be considered as the promising bone healing material.

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