Evaluation of PHBV/calcium silicate composite scaffolds for cartilage tissue engineering

2014 ◽  
Vol 317 ◽  
pp. 278-283 ◽  
Author(s):  
Jun Wu ◽  
Junying Sun ◽  
Jinbo Liu
Keyword(s):  
Tissue Engineering ◽  
Calcium Silicate ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Composite Scaffolds

Composite scaffolds for cartilage tissue engineering

Biorheology ◽  
2008 ◽  
Vol 45 (3-4) ◽  
pp. 501-512 ◽  
Author(s):  
Franklin T. Moutos ◽  
Farshid Guilak
Keyword(s):  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Composite Scaffolds

scholarly journals PLGA/COL I Composite Scaffold Printed by a Low-temperature Deposition Manufacturing Technique for Application to Cartilage Tissue Engineering

2020 ◽  
Author(s):  
Liangquan Peng ◽  
Yong He ◽  
Weimin Zhu ◽  
Wei Lu ◽  
Yong Huang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Low Temperature ◽  
Composite Scaffold ◽  
Cartilage Tissue Engineering ◽  
Three Dimensional ◽  
Cartilage Tissue ◽  
Composite Scaffolds ◽  
Low Temperature Deposition ◽  
Temperature Deposition

Abstract Background Composite scaffolds of poly(lactic-co-glycolic acid) (PLGA) and PLGA/COL I were developed by a low-temperature deposition manufacturing (LDM) technique using three-dimensional printing technology. Their physical properties were tested, and the scaffolds were then used as cell culture platforms to prepare an ideal scaffold for cartilage tissue engineering. Methods The LDM technique was used to fabricate PLGA and PLGA/COL I composite scaffolds. The macrostructure, micromorphology, porosity, hydrophobicity, mechanical properties, and chemical structure of these scaffolds were examined. Primary chondrocytes were isolated and identified, second-passage cells were seeded onto the two scaffolds, and the adhesion and proliferation of the cells were determined. Results Both the PLGA and PLGA/COL I scaffolds prepared by LDM displayed a regular three-dimensional structure with high porosity. The PLGA scaffold had better mechanical properties than the PLGA/COL I scaffold, while the latter had significantly higher hydrophilicity than the former. The PLGA/COL I scaffold cultured with chondrocytes exhibited a higher adhesion rate and proliferation rate than the PLGA/COL I scaffold. Conclusion The novel PLGA/COL I composite scaffold printed by the LDM technique exhibited favourable biocompatibility and biomechanical characteristics and could be a good candidate for cartilage tissue engineering.

scholarly journals 3D-Printed PCL Composite Scaffolds Incorporating IGF-1 Loaded PLGA Nanoparticles for Cartilage Tissue Engineering

2020 ◽  
Author(s):  
Peiran Wei ◽  
Yan Xu ◽  
Yue Gu ◽  
Qingqiang Yao ◽  
Jiayi Li ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Pore Size ◽  
Release Kinetics ◽  
Cartilage Tissue Engineering ◽  
Plga Nanoparticles ◽  
Contact Angles ◽  
Expression Level ◽  
Cartilage Tissue ◽  
Composite Scaffolds ◽  
3D Printed

Abstract Objective: To fabricate and test a 3D-printed PCL scaffold incorporating IGF-1 loaded PLGA nanoparticles for cartilage tissue engineering.Methods: IGF-1 loaded PLGA nanoparticles were produced by the double-emulsion method, and were incorporated onto 3D printed PCL scaffolds via PDA. Particle size, loading effciency (LE) and encapsulation effciency (EE) of the nanoparticles were examined. SEM, pore size, porosity, compression testing, contact angle, IGF-1 release kinetics of the composite scaffolds were also determined. For cell culture studies, CCK-8, Live/dead, MTT, GAG content and expression level of chondrocytes specific genes and HIF-1α were also tested.Results: There was no difference of the nanoparticle size. And the LE and EE of IGF-1 in PLGA nanoparticles was about 5.53%±0.12% and 61.26%±2.71%, respectively. There was a slower, sustained release for all drug-loaded nanoparticles PLGA/PDA/PCL scaffolds. There was no difference of pore size, porosity, compressive strength of each scaffold. The contact angles PCL scaffolds were significant decreased when coated with PDA and PLGA nanoparticales. (P < 0.05) Live/dead staining showed more cells attached to the IGF-1 PLGA/PDA/PCL scaffolds. The CCK-8 and MTT assay showed higher cell proliferation and better biocompatibility of the IGF-1 PLGA/PDA/PCL scaffolds. (P < 0.05) GAG content, chondrogenic gene expression level of SOX-9, COL-II, N-cadh, ACAN, and HIF pathway related gene(HIF-1α) were significantly higher in IGF-1 PLGA/PDA/PCL scaffolds on days 7 and 14 compared to other groups. (P < 0.05)Conclusions: IGF-1 PLGA/PDA/PCL scaffolds may be a better method for sustained IGF-1 administration and a promising scaffold for cartilage tissue engineering.

scholarly journals Hybrid and Composite Scaffolds Based on Extracellular Matrices for Cartilage Tissue Engineering

2019 ◽  
Vol 25 (3) ◽  
pp. 202-224 ◽  
Author(s):  
Mohsen Setayeshmehr ◽  
Ebrahim Esfandiari ◽  
Mohammad Rafieinia ◽  
Batool Hashemibeni ◽  
Asghar Taheri-Kafrani ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Extracellular Matrices ◽  
Cartilage Tissue ◽  
Composite Scaffolds

scholarly journals Mesenchymal stem cells loaded on 3D-printed gradient poly(ε-caprolactone)/methacrylated alginate composite scaffolds for cartilage tissue engineering

2021 ◽  
Vol 8 (3) ◽  
Author(s):  
Yanyan Cao ◽  
Peng Cheng ◽  
Shengbo Sang ◽  
Chuan Xiang ◽  
Yang An ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Morphology ◽  
Chondrogenic Differentiation ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Composite Scaffolds ◽  
Individual Layer ◽  
3D Printed

Abstract Cartilage has limited self-repair ability due to its avascular, alymphatic and aneural features. The combination of three-dimensional (3D) printing and tissue engineering provides an up-and-coming approach to address this issue. Here, we designed and fabricated a tri-layered (superficial layer (SL), middle layer (ML) and deep layer (DL)) stratified scaffold, inspired by the architecture of collagen fibers in native cartilage tissue. The scaffold was composed of 3D printed depth-dependent gradient poly(ε-caprolactone) (PCL) impregnated with methacrylated alginate (ALMA), and its morphological analysis and mechanical properties were tested. To prove the feasibility of the composite scaffolds for cartilage regeneration, the viability, proliferation, collagen deposition and chondrogenic differentiation of embedded rat bone marrow mesenchymal stem cells (BMSCs) in the scaffolds were assessed by Live/dead assay, CCK-8, DNA content, cell morphology, immunofluorescence and real-time reverse transcription polymerase chain reaction. BMSCs-loaded gradient PCL/ALMA scaffolds showed excellent cell survival, cell proliferation, cell morphology, collagen II deposition and hopeful chondrogenic differentiation compared with three individual-layer scaffolds. Hence, our study demonstrates the potential use of the gradient PCL/ALMA construct for enhanced cartilage tissue engineering.

scholarly journals 3D-printed PCL composite scaffolds incorporating IGF-1 loaded PLGA nanoparticles for cartilage tissue engineering

2020 ◽  
Author(s):  
Peiran Wei ◽  
Yan Xu ◽  
Yue Gu ◽  
Qingqiang Yao ◽  
Jiayi Li ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Plga Nanoparticles ◽  
Cartilage Tissue ◽  
Composite Scaffolds ◽  
3D Printed

Abstract The authors have withdrawn this preprint due to author disagreement.

Sign in / Sign up

Export Citation Format

Share Document