scholarly journals Composite scaffolds for cartilage tissue engineering based on natural polymers of bacterial origin, thermoplastic poly(3-hydroxybutyrate) and micro-fibrillated bacterial cellulose

2016 ◽  
Vol 65 (7) ◽  
pp. 780-791 ◽  
Author(s):  
Everest Akaraonye ◽  
Jan Filip ◽  
Mirka Safarikova ◽  
Vehid Salih ◽  
Tajalli Keshavarz ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bacterial Cellulose ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Natural Polymers ◽  
Composite Scaffolds ◽  
Bacterial Origin

scholarly journals Study of bacterial cellulose as scaffold on cartilage tissue engineering

2018 ◽  
Author(s):  
Saharman Gea ◽  
Reka Mustika Sari ◽  
Averroes Fazlurrahman Piliang ◽  
Denny Pratama Indrawan ◽  
Yasir Arafat Hutapea
Keyword(s):  
Tissue Engineering ◽  
Bacterial Cellulose ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue

Composite scaffolds for cartilage tissue engineering

Biorheology ◽  
2008 ◽  
Vol 45 (3-4) ◽  
pp. 501-512 ◽  
Author(s):  
Franklin T. Moutos ◽  
Farshid Guilak
Keyword(s):  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Composite Scaffolds

scholarly journals PLGA/COL I Composite Scaffold Printed by a Low-temperature Deposition Manufacturing Technique for Application to Cartilage Tissue Engineering

2020 ◽  
Author(s):  
Liangquan Peng ◽  
Yong He ◽  
Weimin Zhu ◽  
Wei Lu ◽  
Yong Huang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Low Temperature ◽  
Composite Scaffold ◽  
Cartilage Tissue Engineering ◽  
Three Dimensional ◽  
Cartilage Tissue ◽  
Composite Scaffolds ◽  
Low Temperature Deposition ◽  
Temperature Deposition

Abstract Background Composite scaffolds of poly(lactic-co-glycolic acid) (PLGA) and PLGA/COL I were developed by a low-temperature deposition manufacturing (LDM) technique using three-dimensional printing technology. Their physical properties were tested, and the scaffolds were then used as cell culture platforms to prepare an ideal scaffold for cartilage tissue engineering. Methods The LDM technique was used to fabricate PLGA and PLGA/COL I composite scaffolds. The macrostructure, micromorphology, porosity, hydrophobicity, mechanical properties, and chemical structure of these scaffolds were examined. Primary chondrocytes were isolated and identified, second-passage cells were seeded onto the two scaffolds, and the adhesion and proliferation of the cells were determined. Results Both the PLGA and PLGA/COL I scaffolds prepared by LDM displayed a regular three-dimensional structure with high porosity. The PLGA scaffold had better mechanical properties than the PLGA/COL I scaffold, while the latter had significantly higher hydrophilicity than the former. The PLGA/COL I scaffold cultured with chondrocytes exhibited a higher adhesion rate and proliferation rate than the PLGA/COL I scaffold. Conclusion The novel PLGA/COL I composite scaffold printed by the LDM technique exhibited favourable biocompatibility and biomechanical characteristics and could be a good candidate for cartilage tissue engineering.

scholarly journals 3D-Printed PCL Composite Scaffolds Incorporating IGF-1 Loaded PLGA Nanoparticles for Cartilage Tissue Engineering

2020 ◽  
Author(s):  
Peiran Wei ◽  
Yan Xu ◽  
Yue Gu ◽  
Qingqiang Yao ◽  
Jiayi Li ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Pore Size ◽  
Release Kinetics ◽  
Cartilage Tissue Engineering ◽  
Plga Nanoparticles ◽  
Contact Angles ◽  
Expression Level ◽  
Cartilage Tissue ◽  
Composite Scaffolds ◽  
3D Printed

Abstract Objective: To fabricate and test a 3D-printed PCL scaffold incorporating IGF-1 loaded PLGA nanoparticles for cartilage tissue engineering.Methods: IGF-1 loaded PLGA nanoparticles were produced by the double-emulsion method, and were incorporated onto 3D printed PCL scaffolds via PDA. Particle size, loading effciency (LE) and encapsulation effciency (EE) of the nanoparticles were examined. SEM, pore size, porosity, compression testing, contact angle, IGF-1 release kinetics of the composite scaffolds were also determined. For cell culture studies, CCK-8, Live/dead, MTT, GAG content and expression level of chondrocytes specific genes and HIF-1α were also tested.Results: There was no difference of the nanoparticle size. And the LE and EE of IGF-1 in PLGA nanoparticles was about 5.53%±0.12% and 61.26%±2.71%, respectively. There was a slower, sustained release for all drug-loaded nanoparticles PLGA/PDA/PCL scaffolds. There was no difference of pore size, porosity, compressive strength of each scaffold. The contact angles PCL scaffolds were significant decreased when coated with PDA and PLGA nanoparticales. (P < 0.05) Live/dead staining showed more cells attached to the IGF-1 PLGA/PDA/PCL scaffolds. The CCK-8 and MTT assay showed higher cell proliferation and better biocompatibility of the IGF-1 PLGA/PDA/PCL scaffolds. (P < 0.05) GAG content, chondrogenic gene expression level of SOX-9, COL-II, N-cadh, ACAN, and HIF pathway related gene(HIF-1α) were significantly higher in IGF-1 PLGA/PDA/PCL scaffolds on days 7 and 14 compared to other groups. (P < 0.05)Conclusions: IGF-1 PLGA/PDA/PCL scaffolds may be a better method for sustained IGF-1 administration and a promising scaffold for cartilage tissue engineering.

scholarly journals Hybrid and Composite Scaffolds Based on Extracellular Matrices for Cartilage Tissue Engineering

2019 ◽  
Vol 25 (3) ◽  
pp. 202-224 ◽  
Author(s):  
Mohsen Setayeshmehr ◽  
Ebrahim Esfandiari ◽  
Mohammad Rafieinia ◽  
Batool Hashemibeni ◽  
Asghar Taheri-Kafrani ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Extracellular Matrices ◽  
Cartilage Tissue ◽  
Composite Scaffolds

scholarly journals Preparation and characterization of methacrylated gelatin/bacterial cellulose composite hydrogels for cartilage tissue engineering

2019 ◽  
Vol 7 (2) ◽  
pp. 195-202 ◽  
Author(s):  
Liling Gu ◽  
Tao Li ◽  
Xiongbo Song ◽  
Xianteng Yang ◽  
Senlei Li ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bacterial Cellulose ◽  
Articular Chondrocytes ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
X Ray Diffraction ◽  
Porous Network ◽  
Composite Hydrogels ◽  
Chondrocytic Phenotype

Abstract Methacrylated gelatin (GelMA)/bacterial cellulose (BC) composite hydrogels have been successfully prepared by immersing BC particles in GelMA solution followed by photo-crosslinking. The morphology of GelMA/BC hydrogel was examined by scanning electron microscopy and compared with pure GelMA. The hydrogels had very well interconnected porous network structure, and the pore size decreased from 200 to 10 µm with the increase of BC content. The composite hydrogels were also characterized by swelling experiment, X-ray diffraction, thermogravimetric analysis, rheology experiment and compressive test. The composite hydrogels showed significantly improved mechanical properties compared with pure GelMA. In addition, the biocompatility of composite hydrogels were preliminarily evaluated using human articular chondrocytes. The cells encapsulated within the composite hydrogels for 7 days proliferated and maintained the chondrocytic phenotype. Thus, the GelMA/BC composite hydrogels might be useful for cartilage tissue engineering.

scholarly journals Supermacroprous chitosan–agarose–gelatin cryogels: in vitro characterization and in vivo assessment for cartilage tissue engineering

2010 ◽  
Vol 8 (57) ◽  
pp. 540-554 ◽  
Author(s):  
Sumrita Bhat ◽  
Anuj Tripathi ◽  
Ashok Kumar
Keyword(s):  
Tissue Engineering ◽  
Fluorescent Microscopy ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Laboratory Animals ◽  
Natural Polymers ◽  
Unconfined Compression Test ◽  
Polymeric Scaffold

The study focuses on the synthesis of a novel polymeric scaffold having good porosity and mechanical characteristics synthesized by using natural polymers and their optimization for application in cartilage tissue engineering. The scaffolds were synthesized via cryogelation technology using an optimized ratio of the polymer solutions (chitosan, agarose and gelatin) and cross-linker followed by the incubation at sub-zero temperature (−12°C). Microstructure examination of the chitosan–agarose–gelatine (CAG) cryogels was done using scanning electron microscopy (SEM) and fluorescent microscopy. Mechanical analysis, such as the unconfined compression test, demonstrated that cryogels with varying chitosan concentrations, i.e. 0.5–1% have a high compression modulus. In addition, fatigue tests revealed that scaffolds are suitable for bioreactor studies where gels are subjected to continuous cyclic strain. In order to confirm the stability, cryogels were subjected to high frequency (5 Hz) with 30 per cent compression of their original length up to 1 × 10 5 cycles, gels did not show any significant changes in their mass and dimensions during the experiment. These cryogels have exhibited degradation capacity under aseptic conditions. CAG cryogels showed good cell adhesion of primary goat chondrocytes examined by SEM. Cytotoxicity of the material was checked by MTT assay and results confirmed the biocompatibility of the material. In vivo biocompatibility of the scaffolds was checked by the implantation of the scaffolds in laboratory animals. These results suggest the potential of CAG cryogels as a good three-dimensional scaffold for cartilage tissue engineering.

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