Effect of dietary vitamin E on growth, tissue lipid peroxidation, and liver glutathione level of juvenile hybrid tilapia, Oreochromis niloticus×O. aureus, fed oxidized oil

In a previous study, we found that the extent of dietary n-3 docosahexaenoic acid (DHA)-stimulated tissue lipid peroxidation was less than expected from the relative peroxidizability index of the total tissue lipids in rats with adequate vitamin E nutritional status. This suppression of lipid peroxidation was especially prominent in the liver. To elucidate whether this phenomenon was unique to DHA, we compared the peroxidation effects of n-3 α-linolenic acid (α-LN) and n-3 eicosapentaeonic acid (EPA) with those of DHA in rats. Either α-LN (8·6 % of total energy), EPA (8·2 %), or DHA (8·0 %) and one of two levels of dietary vitamin E (7·5 and 54 mg/kg diet) were fed to rats for 22 d. Levels of conjugated diene, chemiluminescence emission and thiobarbituric acid (TBA)-reactive substance in the liver, kidney, and testis were determined as indicators of lipid peroxidation. In rats fed the DHA diet deficient in vitamin E (7·5 m/g diet), TBA values in the liver, kidney, and testis correlated well with the tissues' relative peroxidizability indices. In rats fed the α-LN diet with an adequate level of vitamin E (54 m/g diet), a close association between relative peroxidizability indices and lipid peroxide levels was observed in all the tissues analysed. However, in rats fed either the EPA diet or the DHA diet with an adequate level of vitamin E, the extent of lipid peroxidation in each tissue was less than expected from the relative peroxidizability index. This suppression was particularly marked in the liver. We concluded that suppression of lipid peroxidation below the relative peroxidizability index was not unique to DHA, but was also seen with EPA, which has five double bonds, in rats with adequate vitamin E nutritional status, but not with α-LN, which has three double bonds.

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Effect of dietary vitamin E level on growth, tissue lipid peroxidation, and erythrocyte fragility of transgenic coho salmon, Oncorhynchus kisutch

Comparative Biochemistry and Physiology Part A Molecular & Integrative Physiology ◽

10.1016/j.cbpb.2004.09.001 ◽

2004 ◽

Vol 139 (2) ◽

pp. 199-204 ◽

Cited By ~ 18

Author(s):

Chen-Huei Huang ◽

David A. Higgs ◽

Shannon K. Balfry ◽

Robert H. Devlin

Keyword(s):

Lipid Peroxidation ◽

Vitamin E ◽

Coho Salmon ◽

Oncorhynchus Kisutch ◽

Dietary Vitamin ◽

Tissue Lipid ◽

Tissue Lipid Peroxidation ◽

Erythrocyte Fragility

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Effects of dietary vitamin E level on growth and tissue lipid peroxidation of soft-shelled turtle, Pelodiscus sinensis (Wiegmann)

Aquaculture Research ◽

10.1111/j.1365-2109.2004.01105.x ◽

2004 ◽

Vol 35 (10) ◽

pp. 948-954 ◽

Cited By ~ 20

Author(s):

Chen-Huei Huang ◽

Way-Yee Lin

Keyword(s):

Lipid Peroxidation ◽

Vitamin E ◽

Dietary Vitamin ◽

Tissue Lipid ◽

Pelodiscus Sinensis ◽

Tissue Lipid Peroxidation

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Re-evaluation of the vitamin E requirements of juvenile tilapia (Oreochromis niloticus ✕ O. aureus)

Animal Science ◽

10.1017/s135772980005205x ◽

2001 ◽

Vol 72 (3) ◽

pp. 529-534 ◽

Cited By ~ 32

Author(s):

S. Y. Shiau ◽

L. F. Shiau

Keyword(s):

Lipid Peroxidation ◽

Vitamin E ◽

Oreochromis Niloticus ◽

Control Diet ◽

Dietary Treatment ◽

Dietary Lipid ◽

Tocopheryl Acetate ◽

Dietary Vitamin ◽

Feeding Trial ◽

Protein Deposition

AbstractA 10-week feeding trial was conducted to re-evaluate the level of dietary vitamin E (DL- α-tocopheryl acetate) that was adequate for juvenile tilapia Oreochromis niloticus ✕ O. aureus given diets containing two dietary lipid concentrations. Purified diets with eight levels of vitamin E (0, 25, 50, 75, 100, 150, 200, 400 mg/kg diet) at either 50 or 120 g lipid per kg were each given to three replicate groups of tilapia (mean weight: 0·69 (s.e.0·02) g) reared in a closed, recirculating system. Food efficiency and protein deposition were significantly (P < 0·05) higher in fish given 50 mg vitamin E per kg diet and 75 mg/kg diet in the 50 and 120 g lipid per kg groups respectively, compared with fish given the unsupplemented control diet. Mortality of fish was not affected by dietary treatment. Weight gain and liver microsomal ascorbic acid-stimulated lipid peroxidation data analysed by broken-line regression indicated that the optimum dietary vitamin E requirements in juvenile tilapia are 42 to 44 mg vitamin E per kg and 60 to 66 mg vitamin E per kg in 50 and 120 g lipid per kg diets, respectively.

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