Increased susceptibility of white spot syndrome virus-exposed Penaeus vannamei to Vibrio parahaemolyticus causing acute hepatopancreatic necrosis disease

Kuruma prawn, Marsupenaeus japonicus, has the third largest annual yield among shrimp species with vital economic significance in China. White spot syndrome virus (WSSV) is a great threat to the global shrimp farming industry and results in high mortality. Pellino, a highly conserved E3 ubiquitin ligase, has been found to be an important modulator of the Toll-like receptor (TLR) signaling pathways that participate in the innate immune response and ubiquitination. In the present study, the Pellino gene from Marsupenaeus japonicus was identified. A qRT-PCR assay showed the presence of MjPellino in all the tested tissues and revealed that the transcript level of this gene was significantly upregulated in both the gills and hemocytes after challenge with WSSV and Vibrio parahaemolyticus. The function of MjPellino was further verified at the protein level. The results of the three-dimensional modeling and protein–protein docking analyses and a GST pull-down assay revealed that the MjPellino protein was able to bind to the WSSV envelope protein VP26. In addition, the knockdown of MjPellino in vivo significantly decreased the expression of MjAMPs. These results suggest that MjPellino might play an important role in the immune response of kuruma prawn.

Download Full-text

Pre-exposure to infectious hypodermal and haematopoietic necrosis virus or to inactivated white spot syndrome virus (WSSV) confers protection against WSSV in Penaeus vannamei (Boone) post-larvae

Journal of Fish Diseases ◽

10.1111/j.1365-2761.2006.00739.x ◽

2006 ◽

Vol 29 (10) ◽

pp. 589-600 ◽

Cited By ~ 19

Author(s):

J Melena ◽

B Bayot ◽

I Betancourt ◽

Y Amano ◽

F Panchana ◽

...

Keyword(s):

White Spot Syndrome Virus ◽

White Spot ◽

Penaeus Vannamei ◽

Necrosis Virus ◽

White Spot Syndrome

Download Full-text

Protector effect of beta-glucans from shrimp pond-related yeasts in Penaeus vannamei rearing under white spot syndrome virus presence

Latin American Journal of Aquatic Research ◽

10.3856/vol49-issue1-fulltext-2514 ◽

2021 ◽

Vol 49 (1) ◽

pp. 18-28

Author(s):

Norma A. Ochoa-Álvarez ◽

Ramón Casillas-Hernández ◽

Francisco J. Magallón-Barajas ◽

Jesús M. Ramirez-Orozco ◽

Elizabeth Carbajal-Millán

Keyword(s):

Protective Effect ◽

White Spot Syndrome Virus ◽

White Spot ◽

Proton Nuclear Magnetic Resonance ◽

Penaeus Vannamei ◽

Marine Yeasts ◽

Shrimp Pond ◽

Beta Glucans ◽

White Spot Syndrome ◽

Excellent Source

This research study tested the protective effect of prolonged exposure of shrimp food supplemented with glucans from shrimp-pond related yeasts on shrimp Penaeus vannamei reared under the presence of the white spot syndrome virus (WSSV). The glucans extracted and purified from isolated marine yeasts identified as Debaryomyces hansenii, Candida tropicalis, Candida humilis, Candida glabrata, Pichia kudriavzevyi, Wickerhamomyces anomalus and the terrestrial Saccharomyces cerevisiae yeast were characterized by the Fourier-transform infrared spectroscopy and proton nuclear magnetic resonance spectroscopy. The treatments were prepared with food enriched with the yeast beta-glucans and the control groups without beta-glucans. Shrimp were fed thrice a day and challenged orally with WSSV on days 31, 54, 66 and muscularly at day 70. The animals were assessed for the protective effect in terms of post-infection total hemocyte counts, and survival rate. The results indicated that marine yeasts possessed ß-1,3/1,6-glucans, and that D. hansenii was an excellent source yielding 30% of its dry biomass of pure glucans. For the positive control group where no glucans were added, WSSV challenges showed 100% survival when the virus was provided orally, and 40% when the virus was injected. These results also indicated that the shrimp line selected for this study was a resistant line for WSSV. Shrimp groups fed with glucans of the marine yeasts D. hansenii and C. humillis showed a significant protection, allowing shrimp survival of 66% while terrestrial yeast showed 57.14%. These results indicated that marine yeasts growing in the shrimp pond were an excellent source of beta-glucans that allowed extra protection against the mortality caused by this pathogenic virus.

Download Full-text