Pre-exposure to infectious hypodermal and haematopoietic necrosis virus or to inactivated white spot syndrome virus (WSSV) confers protection against WSSV in Penaeus vannamei (Boone) post-larvae

2006 ◽  
Vol 29 (10) ◽  
pp. 589-600 ◽  
Author(s):  
J Melena ◽  
B Bayot ◽  
I Betancourt ◽  
Y Amano ◽  
F Panchana ◽  
...  
Keyword(s):  
White Spot Syndrome Virus ◽  
White Spot ◽  
Penaeus Vannamei ◽  
Necrosis Virus ◽  
White Spot Syndrome

scholarly journals Co-Infection of Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV) and White Spot Syndrome Virus (WSSV) in the Wild Crustaceans of Andaman and Nicobar Archipelago, India

Viruses ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1378
Author(s):  
Kandasamy Saravanan ◽  
Jayasimhan Praveenraj ◽  
Rajendran Kiruba-Sankar ◽  
Varsha Devi ◽  
Utpal Biswas ◽  
...  
Keyword(s):  
Inclusion Bodies ◽  
White Spot Syndrome Virus ◽  
Penaeus Monodon ◽  
White Spot ◽  
Scylla Serrata ◽  
Histopathological Analysis ◽  
Necrosis Virus ◽  
High Sequence Identity ◽  
In The Wild ◽  
White Spot Syndrome

The present study was intended to screen the wild crustaceans for co-infection with Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV) and White Spot Syndrome Virus (WSSV) in Andaman and Nicobar Archipelago, India. We screened a total of 607 shrimp and 110 crab samples using a specific polymerase chain reaction, and out of them, 82 shrimps (13.5%) and 5 (4.5%) crabs were found positive for co-infection of IHHNV and WSSV. A higher rate of co-infection was observed in Penaeus monodon and Scylla serrata than other shrimp and crab species. The nucleotide sequences of IHHNV and WSSV obtained from crab in this present study exhibited very high sequence identity with their counterparts retrieved from various countries. Histopathological analysis of the infected shrimp gill sections further confirmed the eosinophilic intra-nuclear cowdry type A inclusion bodies and basophilic intra-nuclear inclusion bodies characteristics of IHHNV and WSSV infections, respectively. The present study serves as the first report on co-infection of WSSV and IHHNV in Andaman and Nicobar Archipelago, India and accentuates the critical need for continuous monitoring of wild crustaceans and appropriate biosecurity measures for brackishwater aquaculture.

scholarly journals Protector effect of beta-glucans from shrimp pond-related yeasts in Penaeus vannamei rearing under white spot syndrome virus presence

2021 ◽  
Vol 49 (1) ◽  
pp. 18-28
Author(s):  
Norma A. Ochoa-Álvarez ◽  
Ramón Casillas-Hernández ◽  
Francisco J. Magallón-Barajas ◽  
Jesús M. Ramirez-Orozco ◽  
Elizabeth Carbajal-Millán
Keyword(s):  
Protective Effect ◽  
White Spot Syndrome Virus ◽  
White Spot ◽  
Proton Nuclear Magnetic Resonance ◽  
Penaeus Vannamei ◽  
Marine Yeasts ◽  
Shrimp Pond ◽  
Beta Glucans ◽  
White Spot Syndrome ◽  
Excellent Source

This research study tested the protective effect of prolonged exposure of shrimp food supplemented with glucans from shrimp-pond related yeasts on shrimp Penaeus vannamei reared under the presence of the white spot syndrome virus (WSSV). The glucans extracted and purified from isolated marine yeasts identified as Debaryomyces hansenii, Candida tropicalis, Candida humilis, Candida glabrata, Pichia kudriavzevyi, Wickerhamomyces anomalus and the terrestrial Saccharomyces cerevisiae yeast were characterized by the Fourier-transform infrared spectroscopy and proton nuclear magnetic resonance spectroscopy. The treatments were prepared with food enriched with the yeast beta-glucans and the control groups without beta-glucans. Shrimp were fed thrice a day and challenged orally with WSSV on days 31, 54, 66 and muscularly at day 70. The animals were assessed for the protective effect in terms of post-infection total hemocyte counts, and survival rate. The results indicated that marine yeasts possessed ß-1,3/1,6-glucans, and that D. hansenii was an excellent source yielding 30% of its dry biomass of pure glucans. For the positive control group where no glucans were added, WSSV challenges showed 100% survival when the virus was provided orally, and 40% when the virus was injected. These results also indicated that the shrimp line selected for this study was a resistant line for WSSV. Shrimp groups fed with glucans of the marine yeasts D. hansenii and C. humillis showed a significant protection, allowing shrimp survival of 66% while terrestrial yeast showed 57.14%. These results indicated that marine yeasts growing in the shrimp pond were an excellent source of beta-glucans that allowed extra protection against the mortality caused by this pathogenic virus.

Sign in / Sign up

Export Citation Format

Share Document