The effects of temperature changes on the isozyme and Hsp70 levels of the Amur sturgeon, Acipenser schrenckii, at two acclimation temperatures

ABSTRACT The effects of temperature, salt concentration, and formamide concentration on the emission characteristics of commonly used fluorescent labels were evaluated on DNA microchips. The emission intensities of different fluorophores without hybridization were observed to vary, each to a different extent, to mainly temperature changes. Rhodamine red, TAMRA (tetramethylrhodamine), and dyes from the carbocyanide group exhibited the largest variations, and Texas Red and Oregon Green exhibited the smallest variations. This temperature dependency was shown to affect results obtained during melting curve analysis in DNA microarray studies. To minimize the bias associated with the temperature-dependent emission of different fluorescent labels, a normalization step was proposed.

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The microeconomic effects of temperature changes

Journal of Cleaner Production ◽

10.1016/j.jclepro.2021.129389 ◽

2021 ◽

pp. 129389

Author(s):

Wenjian He ◽

Yu Cheng ◽

Hongxiao Zhang ◽

Fuqiang Guo ◽

Huimiao Yu

Keyword(s):

Temperature Changes ◽

Effects Of Temperature

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Comparative Effects of Temperature on the Sodium and Water Permeabilities of the Gills of a Stenohaline Freshwater Fish (Carassius Auratus) and a Stenohaline Marine Fish (Serranus Scriba, Serranus Cabrilla)

Journal of Experimental Biology ◽

10.1242/jeb.57.2.359 ◽

1972 ◽

Vol 57 (2) ◽

pp. 359-366 ◽

Cited By ~ 1

Author(s):

J. ISAIA

Keyword(s):

Freshwater Fish ◽

Marine Fish ◽

Osmotic Gradient ◽

Water Fluxes ◽

Simple Diffusion ◽

Temperature Changes ◽

Diffusional Permeability ◽

Effects Of Temperature ◽

Sea Perch ◽

Branchial Membrane

1. Comparative effects of temperature on the permeability of the gill to water and to sodium were studied in the marine sea perch Serranus and the freshwater goldfish Carassius. 2. The acclimation Q10 for the water fluxes is higher in the freshwater fish than in the marine fish. 3. In the goldfish the osmotic permeability (Pos) is greater than the diffusional permeability (Pdlf) at all acclimation temperatures, suggesting the presence of ‘waterfilled channels’ in the branchial membrane. In the sea perch, on the other hand, Pos/Pdlf is approximately 1, indicating that water movements probably occur by simple diffusion. 4. The permeabilities to water and to sodium are similar in the sea perch but very different in the goldfish. Considering these results together with those from a similar study on an elasmobranch, it would seem that the more perfect the semipermeability of the gill membrane and the weaker the transepithelial osmotic gradient, the greater is the branchial porosity. 5. Assuming that temperature changes do not cause modification of the branchial surface or relative permeabilities to water and to sodium the independence of the temperature-coefficient variations for water and for sodium indicates a certain dissociation between the movements of salt and of water, in the sea perch. In the goldfish, assuming a constant branchial surface and in view of the fact that Pos > Pdif the high temperature coefficients for the water fluxes suggest that the water in the ‘water-filled channels’ is in a highly organized state.

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The Effects of Temperature Changes on the Acetylcholine and Neostigmine Induced Contraction of Rabbit Trcheal Smooth Muscle

Korean Journal of Anesthesiology ◽

10.4097/kjae.1999.37.5.913 ◽

1999 ◽

Vol 37 (5) ◽

pp. 913

Author(s):

Oh Kyoung Kwon ◽

Chang Sung Kim ◽

Keon Hee Ryu

Keyword(s):

Smooth Muscle ◽

Temperature Changes ◽

Effects Of Temperature

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Impact of Internal Thermal Insulation Systems on Static Behaviour of Exterior Walls

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.649.11 ◽

2013 ◽

Vol 649 ◽

pp. 11-14 ◽

Cited By ~ 1

Author(s):

Jan Pašek

Keyword(s):

Climatic Conditions ◽

The Czech Republic ◽

Temperature Changes ◽

Analysis And Evaluation ◽

Adjacent Structures ◽

Reduce Heat Loss ◽

Insulation Systems ◽

Effects Of Temperature ◽

Thermal Techniques ◽

Static Behaviour

In general, in the climatic conditions of the Czech Republic the inner insulation of cladding structures is an unsuitable solution to reduce heat loss of building by means of heat transfer. This statement is valid both in terms of building thermal techniques and also in terms of statics. Research focused on the hygrothermal regime of external walls insulated on the inside is very extensive. On the other hand, the systematic attention is not yet paid to detailed analysis of the consequences of such solution on the static behavior of the cladding structures. The paper focuses on analysis and evaluation of stress increase in external walls and adjacent structures caused by non-forced effects of temperature changes in the environment after the application of internal insulation.

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