scholarly journals Isolation and characterization of lymphoid enhancer factor-1-positive deciduous dental pulp stem-like cells after transfection with a piggyBac vector containing LEF1 promoter-driven selection markers

2017 ◽  
Vol 81 ◽  
pp. 110-120 ◽  
Author(s):  
Tomoya Murakami ◽  
Issei Saitoh ◽  
Masahiro Sato ◽  
Emi Inada ◽  
Miki Soda ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Isolation And Characterization ◽  
Piggybac Vector ◽  
Enhancer Factor ◽  
Selection Markers

Isolation and Characterization of Dental Pulp Stem Cells from a Patient with Papillon–Lefèvre Syndrome

2013 ◽  
Vol 39 (1) ◽  
pp. 31-38 ◽  
Author(s):  
Pakize Neslihan Taşlı ◽  
Sıdıka Tapşın ◽  
Sezin Demirel ◽  
Mehmet Emir Yalvaç ◽  
Serap Akyuz ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Isolation And Characterization

Isolation and characterization of SSEA-4-positive subpopulation of human deciduous dental pulp cells

2014 ◽  
Vol 19 (2) ◽  
pp. 363-371 ◽  
Author(s):  
Noriaki Kawanabe ◽  
Hiroaki Fukushima ◽  
Yoshihito Ishihara ◽  
Takeshi Yanagita ◽  
Hiroshi Kurosaka ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Dental Pulp Cells ◽  
Isolation And Characterization ◽  
Pulp Cells

Isolation and characterization of dental pulp stem cells from a supernumerary tooth

2008 ◽  
Vol 37 (9) ◽  
pp. 571-574 ◽  
Author(s):  
Anderson Hsien-Cheng Huang ◽  
Yuk-Kwan Chen ◽  
Lin-Min Lin ◽  
Tien-Yue Shieh ◽  
Anthony Wing-Sang Chan
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Supernumerary Tooth ◽  
Isolation And Characterization

Isolation and Characterization of Human Dental Pulp Stem/Stromal Cells From Nonextracted Crown-fractured Teeth Requiring Root Canal Therapy

2009 ◽  
Vol 35 (5) ◽  
pp. 673-681 ◽  
Author(s):  
Anderson Hsien-Cheng Huang ◽  
Yuk-Kwan Chen ◽  
Anthony Wing-Sang Chan ◽  
Tien-Yu Shieh ◽  
Li-Min Lin
Keyword(s):  
Stromal Cells ◽  
Root Canal ◽  
Dental Pulp ◽  
Root Canal Therapy ◽  
Isolation And Characterization ◽  
Human Dental Pulp

Isolation and Characterization of a Population of Immature Dental Pulp Stem Cells Expressing OCT-4 and Other Embryonic Stem Cell Markers

2006 ◽  
Vol 184 (3-4) ◽  
pp. 105-116 ◽  
Author(s):  
Irina Kerkis ◽  
Alexandre Kerkis ◽  
Dmitri Dozortsev ◽  
Gaëlle Chopin Stukart-Parsons ◽  
Sílvia Maria Gomes Massironi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Dental Pulp ◽  
Embryonic Stem ◽  
Stem Cell Markers ◽  
Cell Markers ◽  
Isolation And Characterization ◽  
Embryonic Stem Cell Markers

scholarly journals Isolation and Characterization of Stem Cells Derived by Human Dental Pulp from Harvest Based in Rotary and Manual Techniques used in Endodontic Therapy

2020 ◽  
Vol 23 (1) ◽  
Author(s):  
Jairo Barros Weiss ◽  
Fernanda Da Silva Gonçalves ◽  
Carlos Magno Da Costa Maranduba ◽  
Leandro Marques De Resende ◽  
Antonio Marcio Resende Do Carmo
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Mtt Assay ◽  
Dental Pulp ◽  
Permanent Teeth ◽  
Pulp Tissue ◽  
Stem Cell Source ◽  
Isolation And Characterization ◽  
The Impact

Objective: The aim of this study was to evaluate the impact on the isolation and characterization of stem cells from pulp tissues obtained through rotary instrumentation techniques compared to the manual technique. Material and Methods: Thirty permanent teeth were included, 15 of which were instrumented with rotational technique (Protaper SX) and other 15 with manual technique. Cells obtained were characterized by flow cytometry and proliferation was evaluated by the MTT assay. The plasticity was evaluated for adipogenic, osteogenic and odontogenic differentiations. Results: Cells isolated from the pulp of permanent teeth, by manual techniques, presented fibroblast morphology and were able to differentiate successfully. All lineages expressed CD29, CD73, CD90, CD105, CD146, CD166 and were negative for CD31, CD34 and CD45. MTT assay showing significantly increased proliferation of hDPSCs in 5 and 7 days of the culture. Conclusion: The present study demonstrated that manual instrumentation technique is one of the best candidates to harvest dental pulp tissue as the dental stem cell source due to ability effective expanded with less tissue invasion. The technique of rotational instrumentation proved to be very harmful to the tissues of the dental pulp, and we can’t obtain cells using this technique.Keywords: Root Canal Therapy; Pulpectomy; Anatomy and Histology; Stem Cell.

scholarly journals Isolation and Characterization of Neural Crest-Derived Stem Cells from Dental Pulp of Neonatal Mice

PLoS ONE ◽  
2011 ◽  
Vol 6 (11) ◽  
pp. e27526 ◽  
Author(s):  
Kajohnkiart Janebodin ◽  
Orapin V. Horst ◽  
Nicholas Ieronimakis ◽  
Gayathri Balasundaram ◽  
Kanit Reesukumal ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Crest ◽  
Dental Pulp ◽  
Neonatal Mice ◽  
Isolation And Characterization

Isolation and Characterization of Dental Pulp Stem Cells from Murine Incisors

2014 ◽  
Vol 14 (4) ◽  
pp. 327-331 ◽  
Author(s):  
M.R. Nur Akmal ◽  
Z.A. Intan Zari ◽  
M.A.W. Rohaya ◽  
S. Sahidan ◽  
Z.A. Zaidah ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Isolation And Characterization
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