piggybac vector
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scholarly journals Corrigendum to “Direct conversion of human pluripotent stem cells into cranial motor neurons using a piggyBac vector” [Stem Cell Res. 29 (2018) 189–196]

2021 ◽  
Vol 53 ◽  
pp. 102296
Author(s):  
Riccardo De Santis ◽  
Maria Giovanna Garone ◽  
Francesca Pagani ◽  
Valeria de Turris ◽  
Silvia Di Angelantonio ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Motor Neurons ◽  
Pluripotent Stem Cells ◽  
Direct Conversion ◽  
Human Pluripotent Stem Cells ◽  
Piggybac Vector

scholarly journals Direct conversion of human pluripotent stem cells into cranial motor neurons using a piggyBac vector

2018 ◽  
Vol 29 ◽  
pp. 189-196 ◽  
Author(s):  
Riccardo De Santis ◽  
Maria Giovanna Garone ◽  
Francesca Pagani ◽  
Valeria de Turris ◽  
Silvia Di Angelantonio ◽  
...  
Keyword(s):  
Stem Cells ◽  
Motor Neurons ◽  
Pluripotent Stem Cells ◽  
Direct Conversion ◽  
Human Pluripotent Stem Cells ◽  
Piggybac Vector

Remobilizing deleted piggyBac vector post-integration for transgene stability in silkworm

2015 ◽  
Vol 290 (3) ◽  
pp. 1181-1189 ◽  
Author(s):  
Feng Wang ◽  
Riyuan Wang ◽  
Yuancheng Wang ◽  
Hanfu Xu ◽  
Lin Yuan ◽  
...  
Keyword(s):  
Transgene Stability ◽  
Piggybac Vector

Stably Express Spider Flagelliform Silk Protein in Bombyx Mori Cell Line by PiggyBac Transposon-Derived Vector

2011 ◽  
Vol 332-334 ◽  
pp. 779-782 ◽  
Author(s):  
Yuan Song Zhang ◽  
Tian Fu Zhao ◽  
Ai Chun Zhao ◽  
Masao Nakagaki
Keyword(s):  
Cell Line ◽  
Blot Analysis ◽  
Spider Silk ◽  
Silk Protein ◽  
Transgenic Silkworm ◽  
Helper Plasmid ◽  
Antibiotic Selection ◽  
Spider Silk Protein ◽  
Piggybac Vector ◽  
Turn Structure

Silkworm BmN4 cells were transfected with the helper plasmid and the piggyBac vector( piggyBac-FLAG) in which was inserted with the spider flagelliform silk expression cassette. Via antibiotic selection, most cells showed stable DsRed-expression. Immuno blot analysis showed that flagelliform silk protein of spider was expressed stably in BmN4 cells. Circular dichroism spectra indicated the existence of β-turn structure in recombinant spider flagelliform protein. The present results suggested that transgenic silkworm directly secreting functional spider silk protein in cocoon by using piggyBac system is feasible.

scholarly journals Pogostick: A New Versatile piggyBac Vector for Inducible Gene Over-Expression and Down-Regulation in Emerging Model Systems

PLoS ONE ◽  
2011 ◽  
Vol 6 (4) ◽  
pp. e18659 ◽  
Author(s):  
Bin Chen ◽  
Steven Hrycaj ◽  
Johannes B. Schinko ◽  
Ondrej Podlaha ◽  
Ernst A. Wimmer ◽  
...  
Keyword(s):  
Model Systems ◽  
Down Regulation ◽  
Over Expression ◽  
Piggybac Vector ◽  
Inducible Gene

Germ-line transformation of the Queensland fruit fly, Bactrocera tryoni, using a piggyBac vector in the presence of endogenous piggyBac elements

Genetica ◽  
2010 ◽  
Vol 139 (1) ◽  
pp. 91-97 ◽  
Author(s):  
K. A. Raphael ◽  
D. C. A. Shearman ◽  
K. Streamer ◽  
J. L. Morrow ◽  
A. M. Handler ◽  
...  
Keyword(s):  
Germ Line ◽  
Fruit Fly ◽  
Bactrocera Tryoni ◽  
Piggybac Vector ◽  
Germ Line Transformation
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